Creation of thermostable and alkaline stable xylanase variants by DNA shuffling

Journal of Biotechnology

Volumn 187, Issue , 2014, Pages 139-146

  Stephens, Dawn Elizabeth ^a   Khan, Faez Iqbal ^a   Singh, Parvesh ^a,b   Bisetty, Krishna ^a   Singh, Suren ^a   Permaul, Kugen ^a  

^a DURBAN UNIVERSITY OF TECHNOLOGY   (South Africa)

^b UNIVERSITY OF KWAZULU NATAL   (South Africa)

Author keywords

DNA shuffling; Stability; Staggered extension process; T. lanuginosus; Xylanase

Indexed keywords

ALKALINITY; AMINO ACIDS; COMPUTER SIMULATION; CONVERGENCE OF NUMERICAL METHODS; DNA; DNA SEQUENCES; MOLECULAR DYNAMICS;

DNA SHUFFLING; MOLECULAR DYNAMICS SIMULATIONS; RANDOM MUTAGENESIS; RECOMBINATION METHODS; STRUCTURAL FEATURE; T. LANUGINOSUS; THERMOMYCES LANUGINOSUS; XYLANASES;

STABILITY; CONVERGENCE OF NUMERICAL METHODS;

AMINO ACID; ARGININE; ENZYME VARIANT; PROTEIN; XYLAN ENDO 1,3 BETA XYLOSIDASE; ENDO 1,4 BETA XYLANASE; AMPICILLIN;

ALKALINITY; ALPHA HELIX; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; DNA SHUFFLING; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME STABILITY; HOMOLOGOUS RECOMBINATION; MOLECULAR DYNAMICS; NONHUMAN; PH; POINT MUTATION; PRIORITY JOURNAL; PROTEIN STRUCTURE; SIMULATION; THERMOMYCES LANUGINOSUS; THERMOSTABILITY; WILD TYPE; AMINO ACID SEQUENCE; ASCOMYCETES; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; PROCEDURES; PROTEIN ENGINEERING; TEMPERATURE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STAGGERED EXTENSION PROCESS;

AMINO ACID SEQUENCE; ASCOMYCOTA; DNA SHUFFLING; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; TEMPERATURE;

EID: 84906350726     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.07.446     Document Type: Article

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