Improvement of the stability and activity of the BPO-A1 haloperoxidase from Streptomyces aureofaciens by directed evolution

Journal of Biotechnology

Volumn 192, Issue Part A, 2014, Pages 248-254

  Yamada, Ryosuke ^a   Higo, Tatsutoshi ^a   Yoshikawa, Chisa ^a   China, Hideyasu ^a   Ogino, Hiroyasu ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Directed evolution; Haloperoxidase; Organic solvent stability; Thermostability

Indexed keywords

AMINO ACIDS; BACTERIA; DIMETHYL SULFOXIDE; DIMETHYLFORMAMIDE; STABILIZATION;

AMINO ACID RESIDUES; DIRECTED EVOLUTION; HALOPEROXIDASE; N-DIMETHYL FORMAMIDE; ORGANIC SOLVENT STABILITIES; PROTEIN MOLECULES; SPECIFIC ACTIVITY; THERMOSTABILITY;

ORGANIC SOLVENTS;

ALCOHOL; AMINO ACID; BACTERIAL ENZYME; BPO A1 HALOPEROXIDASE; DIMETHYL SULFOXIDE; HOMODIMER; METHANOL; MUTANT PROTEIN; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; PEROXIDASE; PROPANOL; UNCLASSIFIED DRUG; CHLORODIMEDONE; CYCLOHEXANONE DERIVATIVE; SOLVENT;

ALPHA HELIX; AMINO ACID SUBSTITUTION; ARTICLE; DIMERIZATION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME LOCALIZATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBUNIT; HEAT TREATMENT; INCUBATION TEMPERATURE; INCUBATION TIME; MOLECULAR EVOLUTION; NONHUMAN; PROTEIN PROTEIN INTERACTION; STREPTOMYCES AUREOFACIENS; SURFACE PROPERTY; WILD TYPE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HEAT; METABOLISM; PLASMID; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION;

STREPTOMYCES AUREOFACIENS;

1-PROPANOL; CYCLOHEXANONES; ENZYME STABILITY; ESCHERICHIA COLI; HOT TEMPERATURE; PEROXIDASES; PLASMIDS; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; SOLVENTS; STREPTOMYCES AUREOFACIENS;

EID: 84918580230     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.10.030     Document Type: Article

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