Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives

Methods

Volumn 101, Issue , 2016, Pages 43-55

  Oceguera Yanez, Fabian ^a   Kim, Shin Il ^a   Matsumoto, Tomoko ^a   Tan, Ghee Wan ^a   Xiang, Long ^a,b   Hatani, Takeshi ^a   Kondo, Takayuki ^a   Ikeya, Makoto ^a   Yoshida, Yoshinori ^a   Inoue, Haruhisa ^a   Woltjen, Knut ^a,c  

^a KYOTO UNIVERSITY   (Japan)

^b IPS Portal Inc   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

AAVS1; CRISPR Cas9; Gene targeting; Human induced pluripotent stem cell (iPSC); Nuclease; TALEN

Indexed keywords

AAVS1 GENE; ARTICLE; CELL DIFFERENTIATION; CELL ENGINEERING; CELL LINEAGE; CONTROLLED STUDY; GENE; GENE DOSAGE; GENE EXPRESSION; GENE LOCUS; GENE TARGETING; GENOTYPE; HUMAN; HUMAN CELL; IN VITRO STUDY; INDUCED PLURIPOTENT STEM CELL; PPP1R12C GENE; PRIORITY JOURNAL; PROMOTER REGION; TRANSGENICS; BIOSYNTHESIS; CELL LINE; CHROMOSOME 19; CRISPR CAS SYSTEM; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PARVOVIRINAE; PHYSIOLOGY; REPORTER GENE; TRANSGENE;

GREEN FLUORESCENT PROTEIN;

BASE SEQUENCE; CELL DIFFERENTIATION; CELL LINE; CHROMOSOMES, HUMAN, PAIR 19; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; GENES, REPORTER; GENETIC ENGINEERING; GENETIC LOCI; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; PARVOVIRINAE; PROMOTER REGIONS, GENETIC; TRANSFECTION; TRANSGENES;

EID: 84951190931     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2015.12.012     Document Type: Article

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