Evaluation of DNA extraction kits and phylogenetic diversity of the porcine gastrointestinal tract based on Illumina sequencing of two hypervariable regions

MicrobiologyOpen

Volumn 5, Issue 1, 2016, Pages 70-82

  Burbach, Katharina ^a   Seifert, Jana ^a   Pieper, Dietmar H ^b   Camarinha Silva, Amélia ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

DNA extraction; Feces; Ileum digesta; Illumina amplicon sequencing; Porcine; T RFLP

Indexed keywords

16S RRNA GENE; AMPLICON; ARTICLE; COMMUNITY STRUCTURE; DNA EXTRACTION; FECES; GASTROINTESTINAL TRACT; GENE STRUCTURE; HYPERVARIABLE REGION; ILEUM; ILLUMINA AMPLICON SEQUENCING; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NONHUMAN; PHYLOGENY; PIG; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RNA GENE; SEQUENCE ANALYSIS; TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ANIMAL; DNA SEQUENCE; EVALUATION STUDY; GENETICS; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROCEDURES;

BACTERIAL DNA; RNA 16S;

ANIMALS; BASE SEQUENCE; DNA, BACTERIAL; FECES; ILEUM; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SWINE;

EID: 84948843936     PISSN: None     EISSN: 20458827     Source Type: Journal    
DOI: 10.1002/mbo3.312     Document Type: Article

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