Erratum : Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module (Nature Cell Biology (2015) DOI:10.1038/ncb3241);Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module

Nature Cell Biology

Volumn 17, Issue 11, 2015, Pages 1422-1434

  Roostalu, Johanna ^a   Cade, Nicholas I ^a   Surrey, Thomas ^a  

^a THE FRANCIS CRICK INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

KARYOPHERIN; KARYOPHERIN ALPHA; KARYOPHERIN BETA; MICROTUBULE ASSOCIATED PROTEIN; PROTEIN CHTOG; PROTEIN TPX2; UNCLASSIFIED DRUG; CELL CYCLE PROTEIN; CKAP5 PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; NUCLEAR PROTEIN; TPX2 PROTEIN, HUMAN;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; BIOTINYLATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEPOLYMERIZATION; HUMAN; IN VITRO STUDY; INSECT CELL; LIFESPAN; MICROSCOPY; MICROTUBULE; MICROTUBULE ASSEMBLY; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; ANIMAL; CELL LINE; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM; PROCEDURES; TIME LAPSE IMAGING;

ALPHA KARYOPHERINS; ANIMALS; BETA KARYOPHERINS; CELL CYCLE PROTEINS; CELL LINE; GREEN FLUORESCENT PROTEINS; HUMANS; KARYOPHERINS; MICROSCOPY, FLUORESCENCE; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; NUCLEAR PROTEINS; TIME-LAPSE IMAGING;

EID: 84946478790     PISSN: 14657392     EISSN: 14764679     Source Type: Journal    
DOI: 10.1038/ncb3265     Document Type: Erratum

References (68)

1. Helmke K. J., Heald R., & Wilbur J. D. Interplay between spindle architecture and function. Int. Rev. Cell Mol. Biol. 306, 83-125 (2013
2. Gard D. L., & Kirschner M. W. A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end. J. Cell. Biol. 105, 2203-2215 (1987
3. Brouhard G. J., et al. XMAP215 is a processive microtubule polymerase. Cell 132, 79-88 (2008
4. Kronja I., Kruljac-Letunic A., Caudron-Herger M., Bieling P., & Karsenti E. XMAP215-EB1 interaction is required for proper spindle assembly and chromosome segregation in Xenopus egg extract. Mol. Biol. Cell 20, 2684-2696 (2009
5. Reber S. B., et al. XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules. Nat. Cell Biol. 15, 1116-1122 (2013
6. Gergely F., Draviam V. M., & Raff J. W. The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. Genes Dev. 17, 336-341 (2003
7. Cassimeris L., & Morabito J. TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly. Mol. Biol. Cell 15, 1580-1590 (2004
8. Heald R., et al. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382, 420-425 (1996
9. Carazo-Salas R. E., et al. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. Nature 400, 178-181 (1999
10. Ohba T., Nakamura M., Nishitani H., & Nishimoto T. Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran. Science 284, 1356-1358 (1999
11. Kalab P., Pu R. T., & Dasso M. The ran GTPase regulates mitotic spindle assembly. Curr. Biol. 9, 481-484 (1999
12. Wilde A., & Zheng Y. Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran. Science 284, 1359-1362 (1999
13. Kalab P., Weis K., & Heald R. Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science 295, 2452-2456 (2002
14. Kalab P., Pralle A., Isacoff E. Y., Heald R., & Weis K. Analysis of a RanGTPregulated gradient in mitotic somatic cells. Nature 440, 697-701 (2006
15. Caudron M., Bunt G., Bastiaens P., & Karsenti E. Spatial coordination of spindle assembly by chromosome-mediated signaling gradients. Science 309, 1373-1376 (2005
16. Goshima G., Mayer M., Zhang N., Stuurman N., & Vale R. D. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J. Cell Biol. 181, 421-429 (2008
17. Petry S., Groen A. C., Ishihara K., Mitchison T. J., & Vale R. D. Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2. Cell 152, 768-777 (2013
18. Wittmann T., Wilm M., Karsenti E., & Vernos I. TPX2, a novel xenopus MAP involved in spindle pole organization. J. Cell Biol. 149, 1405-1418 (2000
19. Gruss O. J., et al. Ran induces spindle assembly by reversing the inhibitory effect of importin on TPX2 activity. Cell 104, 83-93 (2001
20. Gruss O. J., et al. Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nat. Cell Biol. 4, 871-879 (2002
21. Aguirre-Portoles C., et al. Tpx2 controls spindle integrity, genome stability, and tumor development. Cancer Res. 72, 1518-1528 (2012
22. Perez de Castro I., & Malumbres M. Mitotic stress and chromosomal instability in cancer: the case for TPX2. Genes Cancer 3, 721-730 (2012
23. Schatz C. A., et al. Importin-regulated nucleation of microtubules by TPX2. EMBO J. 22, 2060-2070 (2003
24. Giesecke A., & Stewart M. Novel binding of the mitotic regulator TPX2 (target protein for Xenopus kinesin-like protein 2) to importin. J. Biol. Chem. 285, 17628-17635 (2010
25. Trieselmann N., Armstrong S., Rauw J., & Wilde A. Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation. J. Cell Sci. 116, 4791-4798 (2003
26. Brunet S., et al. Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts. Mol. Biol. Cell 15, 5318-5328 (2004
27. Tanenbaum M. E., et al. Kif15 cooperates with eg5 to promote bipolar spindle assembly. Curr. Biol. 19, 1703-1711 (2009
28. Vanneste D., Takagi M., Imamoto N., & Vernos I. The role of Hklp2 in the stabilization and maintenance of spindle bipolarity. Curr. Biol. 19, 1712-1717 (2009
29. Koffa M. D., et al. HURP is part of a Ran-dependent complex involved in spindle formation. Curr. Biol. 16, 743-754 (2006
30. Wittmann T., Boleti H., Antony C., Karsenti E., & Vernos I. Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubuleassociated protein, and dynein. J. Cell Biol. 143, 673-685 (1998
31. Ma N., Titus J., Gable A., Ross J. L., & Wadsworth P. TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle. J. Cell Biol. 195, 87-98 (2011
32. Helmke K. J., & Heald R. TPX2 levels modulate meiotic spindle size and architecture in Xenopus egg extracts. J. Cell Biol. 206, 385-393 (2014
33. Tsai M. Y., et al. A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat. Cell Biol. 5, 242-248 (2003
34. Bayliss R., Sardon T., Vernos I., & Conti E. Structural basis of Aurora-A activation by TPX2 at the mitotic spindle. Mol. Cell 12, 851-862 (2003
35. Kufer T. A., et al. Human TPX2 is required for targeting Aurora-A kinase to the spindle. J. Cell Biol. 158, 617-623 (2002
36. Brunet S., et al. Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. PLoS ONE 3, e3338 (2008
37. Groen A. C., Maresca T. J., Gatlin J. C., Salmon E. D., & Mitchison T. J. Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly. Mol. Biol. Cell 20, 2766-2773 (2009
38. Bird A. W., & Hyman A. A. Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. J. Cell Biol. 182, 289-300 (2008
39. Scrofani J., Sardon T., Meunier S., & Vernos I. Microtubule nucleation in mitosis by a RanGTP-dependent protein complex. Curr. Biol. 25, 131-140 (2015
40. Kollman J. M., Merdes A., Mourey L., & Agard D. A. Microtubule nucleation by-tubulin complexes. Nat. Rev. Mol. Cell Biol. 12, 709-721 (2011
41. Bieling P., Telley I. A., Hentrich C., Piehler J., & Surrey T. Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and CTIP dynamics. Methods Cell Biol. 95, 555-580 (2010
42. Al-Bassam J., et al. Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase. Mol. Biol. Cell 23, 2878-2890 (2012
43. Podolski M., Mahamdeh M., & Howard J. Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency. J. Biol. Chem. 289, 28087-28093 (2014
44. Li W., et al. EB1 promotes microtubule dynamics by recruiting Sentin in drosophila cells. J. Cell Biol. 193, 973-983 (2011
45. Wieczorek M., Bechstedt S., Chaaban S., & Brouhard G. J. Microtubuleassociated proteins control the kinetics of microtubule nucleation. Nat. Cell Biol. 17, 907-916 (2015
46. Bieling P., et al. CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites. J. Cell Biol. 183, 1223-1233 (2008
47. Bieling P., et al. Reconstitution of a microtubule plus-end tracking system in vitro. Nature 450, 1100-1105 (2007
48. Maurer S. P., et al. EB1 accelerates two conformational transitions important for microtubule maturation and dynamics. Curr. Biol. 24, 372-384 (2014
49. Chretien D., Fuller S. D., & Karsenti E. Structure of growing microtubule ends: two-dimensional sheets close into tubes at variable rates. J. Cell Biol. 129, 1311-1328 (1995
50. Maurer S. P., Bieling P., Cope J., Hoenger A., & Surrey T. GTPS microtubules mimic the growing microtubule end structure recognized by end-binding proteins (EBs). Proc. Natl Acad. Sci. USA 108, 3988-3993 (2011
51. Ghosh S., Hentrich C., & Surrey T. Micropattern-controlled local microtubule nucleation, transport, and mesoscale organization. ACS Chem. Biol. 8, 673-678 (2013
52. Voter W. A., & Erickson H. P. The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism. J. Biol. Chem. 259, 10430-10438 (1984
53. Wang H. W., Long S., Finley K. R., & Nogales E. Assembly of GMPCPP-bound tubulin into helical ribbons and tubes and effect of colchicine. Cell Cycle 4, 1157-1160 (2005
54. Mozziconacci J., Sandblad L., Wachsmuth M., Brunner D., & Karsenti E. Tubulin dimers oligomerize before their incorporation into microtubules. PLoS ONE 3, e3821 (2008
55. van Breugel M., Drechsel D., & Hyman A. Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer. J. Cell Biol. 161, 359-369 (2003
56. Hyman A. A., Salser S., Drechsel D. N., Unwin N., & Mitchison T. J. Role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP. Mol. Biol. Cell 3, 1155-1167 (1992
57. Hyman A. A., Chretien D., Arnal I., & Wade R. H. Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-methylene-diphosphonate. J. Cell Biol. 128, 117-125 (1995
58. Alushin G. M., et al. High-resolution microtubule structures reveal the structural transitions in-tubulin upon GTP hydrolysis. Cell 157, 1117-1129 (2014
59. Bechstedt S., Lu K., & Brouhard G. J. Doublecortin recognizes the longitudinal curvature of the microtubule end and lattice. Curr. Biol. 24, 2366-2375 (2014
60. Ayaz P., Ye X., Huddleston P., Brautigam C. A., & Rice L. M. A TOG:-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase. Science 337, 857-860 (2012
61. Coombes C. E., Yamamoto A., Kenzie M. R., Odde D. J., & Gardner M. K. Evolving tip structures can explain age-dependent microtubule catastrophe. Curr. Biol. 23, 1342-1348 (2013
62. Tsai M. Y., & Zheng Y. Aurora A kinase-coated beads function as microtubuleorganizing centers and enhance RanGTP-induced spindle assembly. Curr. Biol. 15, 2156-2163 (2005
63. Zacharias D. A., Violin J. D., Newton A. C., & Tsien R. Y. Partitioning of lipidmodified monomeric GFPs into membrane microdomains of live cells. Science 296, 913-916 (2002
64. Snapp E. L., et al. Formation of stacked ER cisternae by low affinity protein interactions. J. Cell Biol. 163, 257-269 (2003
65. Duellberg C., et al. Reconstitution of a hierarchical CTIP interaction network controlling microtubule end tracking of dynein. Nat. Cell Biol. 16, 804-811 (2014
66. Castoldi M., & Popov A. V. Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer. Protein Expr. Purif. 32, 83-88 (2003
67. Hyman A., et al. Preparation of modified tubulins. Methods Enzymol. 196, 478-485 (1991
68. Greenfeld M., Pavlichin D.S., Mabuchi H., & Herschlag D. Single Molecule Analysis Research Tool (SMART): an integrated approach for analyzing single molecule data. PLoS One 7, e30024 (2012