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2, 0.1% Triton X-100, and 3% bovine serum albumin] for 15 min, and stained with the indicated primary antibodies and then with the secondary antibodies, fluorescein isothiocyanate-conjugated goat antibody to mouse immunoglobulin G (IgG) (Biosource, Camarillo, CA) or Rhodamine-conjugated goat antibody to rabbit IgG (CAPPEL, Aurora, OH). Sperm chromatin was stained with Hoechst 33342.
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We thank H. Kobayashi for preparing Xenopus sperm, Y. Zheng for antibodies to Xgrip109, A. Merdes and W. Cleveland for antibodies to NuMA, and O. Martin for help with English. Supported by Grants-in-Aid for Specially Promoted Research of Japan and by the Human Frontier Science Program.
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