Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization

Science

Volumn 350, Issue 6259, 2015, Pages 404-409

  Zhang, Liman ^a,b   Chen, Shuobing ^c,d   Ruan, Jianbin ^a,b   Wu, Jiayi ^c,d   Tong, Alexander B ^a,b   Yin, Qian ^a,b   Li, Yang ^a,b   David, Liron ^a,b   Lu, Alvin ^a,b   Wang, Wei Li ^a,d   Marks, Carolyn ^e   Ouyang, Qi ^c   Zhang, Xinzheng ^f   Mao, Youdong ^a,c,d   Wu, Hao ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c PEKING UNIVERSITY   (China)

^d INTEL CORPORATION   (United States)

^e HARVARD UNIVERSITY   (United States)

^f INSTITUTE OF BIOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; APOPTOSOME; APOPTOTIC PROTEASE ACTIVATING FACTOR 1; BACTERIAL PROTEIN; INFLAMMASOME; INTERLEUKIN 1BETA CONVERTING ENZYME; MULTIPROTEIN COMPLEX; NLR FAMILY APOPTOSIS INHIBITORY PROTEIN 2; NLR FAMILY CARD CONTAINING PROTEIN 4; PEPTIDES AND PROTEINS; PRGJ PROTEIN; UNCLASSIFIED DRUG; APOPTOSIS REGULATORY PROTEIN; CALCIUM BINDING PROTEIN; CASPASE RECRUITMENT DOMAIN SIGNALING PROTEIN; IPAF PROTEIN, MOUSE; NAIP2 PROTEIN, MOUSE; NEURONAL APOPTOSIS INHIBITORY PROTEIN;

APOPTOSIS; BACTERIUM; ELECTRON MICROSCOPY; ENZYME ACTIVITY; IMMUNITY; INNATE BEHAVIOR; LIGAND; POLYMERIZATION; PROTEIN;

ARTICLE; CONFORMATIONAL TRANSITION; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; DIMERIZATION; DISSOCIATION CONSTANT; ENZYME ACTIVATION; GENE MUTATION; OLIGOMERIZATION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SURFACE AREA; AMINO ACID SEQUENCE; ANIMAL; APOPTOSIS; CHEMISTRY; IMMUNOLOGY; INNATE IMMUNITY; MOLECULAR GENETICS; MOUSE; PROTEIN TERTIARY STRUCTURE;

BACTERIA (MICROORGANISMS);

AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; BACTERIAL PROTEINS; CALCIUM-BINDING PROTEINS; CARD SIGNALING ADAPTOR PROTEINS; CASPASE 1; CRYOELECTRON MICROSCOPY; IMMUNITY, INNATE; INFLAMMASOMES; MICE; MOLECULAR SEQUENCE DATA; NEURONAL APOPTOSIS-INHIBITORY PROTEIN; POLYMERIZATION; PROTEIN INTERACTION MAPS; PROTEIN STRUCTURE, TERTIARY;

EID: 84944747007     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aac5789     Document Type: Article

References (30)

1. J. D. Growney, W. F. Dietrich, Genome Res. 10, 1158-1171 (2000).
2. E. M. Kofoed, R. E. Vance, Nature 477, 592-595 (2011).
3. E. Diez et al., Nat. Genet. 33, 55-60 (2003).
4. Y. Zhao et al., Nature 477, 596-600 (2011).
5. J. Yang, Y. Zhao, J. Shi, F. Shao, Proc. Natl. Acad. Sci. U.S.A. 110, 14408-14413 (2013).
6. E. A. Miao et al., Nat. Immunol. 7, 569-575 (2006).
7. E. A. Miao et al., Nat. Immunol. 11, 1136-1142 (2010).
8. L. Franchi et al., Nat. Immunol. 13, 449-456 (2012).
9. B. Hu et al., Proc. Natl. Acad. Sci. U.S.A. 107, 21635-21640 (2010).
10. A. M. Janowski, R. Kolb, W. Zhang, F. S. Sutterwala, Front. Immunol. 4, 370 (2013).
11. J. Garaude, A. Kent, N. van Rooijen, J. M. Blander, Sci. Transl. Med. 4, 120ra16 (2012).
12. S. W. Canna et al., Nat. Genet. 46, 1140-1146 (2014).
13. A. Kitamura, Y. Sasaki, T. Abe, H. Kano, K. Yasutomo, J. Exp. Med. 211, 2385-2396 (2014).
14. N. Romberg et al., Nat Genet. 46, 1135-1139 (2014).
15. E. F. Halff et al., J. Biol. Chem. 287, 38460-38472 (2012).
16. Z. Hu et al., Science 341, 172-175 (2013).
17. T. F. Reubold, S. Wohlgemuth, S. Eschenburg, J. Biol. Chem. 284, 32717-32724 (2009).
18. Q. Bao, W. Lu, J. D. Rabinowitz, Y. Shi, Mol. Cell 25, 181-192 (2007).
19. M. Proell, S. J. Riedl, J. H. Fritz, A. M. Rojas, R. Schwarzenbacher, PLOS ONE 3, e2119 (2008).
20. M. A. Martí-Renom et al., Annu. Rev. Biophys. Biomol. Struct. 29, 291-325 (2000).
21. S. Yuan, C. W. Akey, Structure 21, 501-515 (2013).
22. S. Yuan et al., Structure 19, 128-140 (2011).
23. S. Qi et al., Cell 141, 446-457 (2010).
24. A. Lu et al., Cell 156, 1193-1206 (2014).
25. Z. Hu et al., Science 350, 399-404 (2015).
26. J. L. Tenthorey, E. M. Kofoed, M. D. Daugherty, H. S. Malik, R. E. Vance, Mol. Cell 54, 17-29 (2014).
27. H. Wu, Cell 153, 287-292 (2013).
28. B. S. Franklin et al., Nat. Immunol. 15, 727-737 (2014).
29. X. Cai et al., Cell 156, 1207-1222 (2014).
30. A. Baroja-Mazo et al., Nat. Immunol. 15, 738-748 (2014).