Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance

FEMS Microbiology Letters

Volumn 362, Issue 3, 2015, Pages

  Ding, Jun ^a,b   Holzwarth, Garrett ^a   Penner, Michael H ^a   Patton Vogt, Jana ^c   Bakalinsky, Alan T ^a  

^a OREGON STATE UNIVERSITY   (United States)

^b Medolac Laboratories   (United States)

^c DUQUESNE UNIVERSITY   (United States)

Author keywords

Acetic acid; Acetyl CoA synthetase; Acs2; Lignocellulose; Saccharomyces cerevisiae

Indexed keywords

ACETYL COENZYME A SYNTHETASE; ADENOSINE TRIPHOSPHATE; ACETIC ACID; ACETYL COENZYME A;

ACS2 GENE; AEROBIC FERMENTATION; ANAEROBIC FERMENTATION; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DETOXIFICATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; FUNGUS GROWTH; GENE MUTATION; GENE OVEREXPRESSION; NONHUMAN; NUTRIENT UPTAKE; OPEN READING FRAME; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; FERMENTATION; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PHYSIOLOGY; PLASMID; REAL TIME POLYMERASE CHAIN REACTION;

ACETATE-COA LIGASE; ACETIC ACID; ACETYL COENZYME A; FERMENTATION; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; PLASMIDS; REAL-TIME POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 84944155176     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1093/femsle/fnu042     Document Type: Article

References (39)

1. Aden A, Ruth M, Ibsen K, et al. Lignocellulosic biomass to ethanol process design and economics utilizing cocurrent dilute acid prehydrolysis and enzymatic hydrolysis for corn stover. Technical report NREL/TP-510-32438, 2002. http://www.nrel.gov/docs/fy02osti/32438.pdf
2. Almeida B, Ohlmeier S, Almeida AJ, et al. Yeast protein expression profile during acetic acid-induced apoptosis indicates causal involvement of the TOR pathway. Proteomics 2009;9:720-32.
3. Baranwal S, Azad GK, Singh V, et al. Signaling of chloroquineinduced stress in the yeast Saccharomyces cerevisiae requires the Hog1 and Slt2 mitogen-activated protein kinase pathways. Antimicrob Agents Ch 2014;58:5552-66.
4. Berg P. Acyl adenylates: an enzymatic mechanism of acetate activation. J Biol Chem 1956;222:991-1013.
5. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
6. Chen Y, Daviet L, Schalk M, et al. Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng 2013;15:48-54.
7. Chen F, Zhou J, Shi Z, et al. Effect of acetyl-CoA synthase gene overexpression on physiological function of Saccharomyces cerevisiae. Acta Microbiol Sin 2010;50:1172-9.
8. De Jong-Gubbels P, van den Berg MA, Luttik MA, et al. Overproduction of acetyl-coenzyme A synthetase isoenzymes in respiring Saccharomyces cerevisiae cells does not reduce acetate production after exposure to glucose excess. FEMS Microbiol Lett 1998;165:15-20.
9. De Virgilio C, Bürckert N, Barth G, et al. Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast 1992;8:1043-51.
10. Del Río JC, Marques G, Rencoret J, et al. Occurrence of naturally acetylated lignin units. J Agr Food Chem 2007;55:5461-8.
11. Ding J, Bierma J, Smith MR, et al. Acetic acid inhibits nutrient uptake in Saccharomyces cerevisiae: auxotrophy confounds the use of yeast deletion libraries for strain improvement. Appl Microbiol Biot 2013;97:7405-16.
12. Fang F, Salmon K, Shen MWY, et al. A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Yeast 2011;28:123-36.
13. Frenkel EP, Kitchens RL. Purification and properties of acetyl coenzyme A synthetase from bakers' yeast. J Biol Chem 1977;252:504-7.
14. Gietz RD, Schiestl RH, Willems AR, et al. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 1995;11:355-60.
15. Guaragnella N, Antonacci L, Giannattasio S, et al. Catalase T and Cu, Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae. FEBS Lett 2008;582:210-4.
16. Hueso G, Aparicio-Sanchis R, Montesinos C, et al. A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress. Biochem J 2012;441:255-64.
17. Kawahata M, Masaki K, Fujii T, et al. Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p. FEMS Yeast Res 2006;6:924-36.
18. Klinke HB, Thomsen AB, Ahring BK. Inhibition of ethanolproducing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl Microbiol Biot 2004;66:10-26.
19. Kozak BU, van Rossum HM, Luttik MAH, et al. Engineering acetyl coenzyme A supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of Saccharomyces cerevisiae. mBio 2014;5:e01696-14.
20. Liu X, Zhang X, Zhang Z. Point mutation of H3/H4 histones affects acetic acid tolerance in Saccharomyces cerevisiae. J Biotechnol 2014;187:116-23.
21. Ludovico P, Rodrigues F, Almeida A, et al. Cytochrome c release andmitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae. Mol Biol Cell 2002;13:2598-606.
22. Medina VG, Almering MJH, van Maris AJA, et al. Elimination of glycerol production in anaerobic cultures of a Saccharomyces cerevisiae strain engineered to use acetic acid as an electron acceptor. Appl Environ Microb 2010;76:190-5.
23. Mollapour M, Piper PW. Hog1 mitogen-activated protein kinase phosphorylation targets the yeast Fps1 aquaglyceroporin for endocytosis, thereby rendering cells resistant to acetic acid. Mol Cell Biol 2007;27:6446-56.
24. MülledarM, Capuoano F, Pir P, et al. A prototrophic deletionmutant collection for yeast metabolomics and systems biology. Nature Biotechnol 2012;30:1176-8.
25. Pampulha ME, Loureiro-Dias MC. Energetics of the effect of acetic acid on growth of Saccharomyces cerevisiae. FEMS Microbiol Lett 2000;184:69-72.
26. PfafflMW. A newmathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001;29:e45.
27. 2+ and mitochondrial K+ acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation. Appl Environ Microb 2000;66: 3151-9.
28. Runguphan W, Keasling JD. Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals. Metab Eng 2014;21:103-13.
29. Satyanarayana T, Klein HP. Studies on acetyl-Coenzyme A synthetase of yeast: inhibition by long-chain acyl-Coenzyme A esters. J Bacteriol 1973;115:600-6.
30. Satyanarayana T, Mandel AD, Klein HP. Evidence for two immunologically distinct acetyl-co-enzyme A synthetase in yeast. Biochim Biophys Acta 1974;341:396-401.
31. Scalcinati G, Partow S, Siewers V, et al. Combined metabolic engineering of precursor and co-factor supply to increase a- santalene production by Saccharomyces cerevisiae. Microb Cell Fact 2012;11:117.
32. Shiba Y, Paradise EM, Kirby J, et al. Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids. Metab Eng 2007;9: 160-8.
33. Sierkstra LN, Verbakel JM, Verrips CT. Analysis of transcription and translation of glycolytic enzymes in glucose-limited continuous cultures of Saccharomyces cerevisiae. J Gen Microbiol 1992;138:2559-66.
34. Swinnen S, Fernańdez Niño M, González-Ramos D, et al. The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae. FEMS Yeast Res 2014;14: 642-53.
35. Tanaka K, Ishii Y, Ogawa J, et al. Enhancement of acetic acid tolerance in Saccharomyces cerevisiae by overexpression of the HAA1 gene, encoding a transcriptional activator. Appl Environ Microb 2012;78:8161-3.
36. Van den Berg MA, Jong-Gubbels Pde, Kortland CJ, et al. The two acetyl-coenzyme a synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation. J Biol Chem 1996;271:28953-9.
37. Van den Berg MA, Steensma HY. ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose. Eur J Biochem 1995;231:704-13.
38. Wei N, Quarterman J, Kim SR, et al. Enhanced biofuel production through coupled acetic acid and xylose consumption by engineered yeast. Nat Commun 2013;4:2580.
39. Xu X, Wightman JD, Geller BL, et al. Isolation and characterization of sulfite mutants of Saccharomyces cerevisiae. Curr Genet 1994;25:488-96.