Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli

Journal of Bioscience and Bioengineering

Volumn 120, Issue 5, 2015, Pages 591-595

  Tsukuda, Miyuki ^a,b   Nakashima, Nobutaka ^c   Miyazaki, Kentaro ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Antisense RNA; Antitoxin; Counterselection; Escherichia coli; MqsA; MqsR MqsA; RnlA RnlB; RnlB; Toxin antitoxin

Indexed keywords

CELL PROLIFERATION; CURING; DNA; GENES; GENETIC ENGINEERING; RNA; TOXIC MATERIALS;

ANTISENSE RNA; ANTITOXIN; COUNTERSELECTION; MQSA; MQSR/MQSA; RNLA/RNLB; RNLB; TOXIN-ANTITOXIN;

ESCHERICHIA COLI;

ANTITOXIN; COMPLEMENTARY RNA; BACTERIAL TOXIN; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; GENETIC MARKER; MQSA PROTEIN, E COLI; MQSR PROTEIN, E COLI; RNLA PROTEIN, E COLI; RNLB PROTEIN, E COLI;

ARTICLE; BACTERIAL GENOME; CELL GROWTH; CONTROLLED STUDY; COUNTERSELECTION; ESCHERICHIA COLI; GENE EXPRESSION; GENE REPRESSION; GENE SILENCING; GENETIC ENGINEERING; NONHUMAN; PLASMID; ANALYSIS; BIOSYNTHESIS; CYTOLOGY; DEFICIENCY; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PROCEDURES; PROMOTER REGION;

ANTITOXINS; BACTERIAL TOXINS; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE SILENCING; GENETIC ENGINEERING; GENETIC MARKERS; PLASMIDS; PROMOTER REGIONS, GENETIC; RNA, ANTISENSE;

EID: 84943360282     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2015.03.008     Document Type: Article

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