Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli

Journal of Bacteriology

Volumn 183, Issue 19, 2001, Pages 5523-5528

  Sugai, R ^a   Shimizu, H ^a   Nishiyama, K I ^a   Tokuda, H ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COLD SHOCK PROTEIN; GENE PRODUCT; MUTANT PROTEIN; PROTEIN FABA6; PROTEIN GNSA; PROTEIN GNSB; PROTEIN SECG; UNCLASSIFIED DRUG; UNSATURATED FATTY ACID;

ARTICLE; BACTERIAL MUTATION; COLD SENSITIVITY; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE EXPRESSION REGULATION; GENE ISOLATION; GENE MUTATION; GENE OVEREXPRESSION; GENE TRANSLOCATION; LOW TEMPERATURE PROCEDURES; MEMBRANE FLUIDITY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SUPPRESSOR GENE; TEMPERATURE DEPENDENCE;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; COLD; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACIDS, UNSATURATED; GENE EXPRESSION REGULATION, BACTERIAL; GENES, SUPPRESSOR; MEMBRANE PROTEINS; MUTATION; PROTEIN PRECURSORS; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034829685     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.19.5523-5528.2001     Document Type: Article

References (42)

1. A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery
2. Fatty acid metabolism in sn-glycerol-3-phosphate acyltransferase (plsB) mutants
3. Molecular biology of bacterial membrane lipids
4. Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli
5. Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes
6. Biogenesis of the gram-negative bacterial envelope
7. The SecDFyajC domain of preprotein transtocase controls preprotein movement by regulating SecA membrane cycling
8. SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF
9. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
10. SecG function and phospholipid metabolism in Escherichia coli
11. SecG is an auxiliary component of the protein export apparatus of Escherichia coli
12. Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli
13. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin
14. The major pathways of protein translocation across membranes
15. Differences in fatty acid composition among phosphatidylethanolamine, phosphatidylglycerol and cardiolipin of Escherichia coli
16. Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in a secG deletion mutant of Escherichia coli at low temperature
17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
18. The ATPase activity of SecA is regulated by acidic phospholipids, SecY and the leader and mature domains of precursor proteins
19. Regulation of fatty acid biosynthesis in Escherichia coli
20. Escherichia coli translocase: The unravelling of a molecular machine
21. A mutation in secY that causes enhanced SecA insertion and impaired late functions in protein translocation
22. SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane
23. Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation
24. Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coil at low temperature
25. The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli
26. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation
27. Penicillin-binding protein 2 is essential in wild-type Escherichia coli but not in lov or cya mutants
28. In vitro insertional mutagenesis with a selectable DNA fragment
29. The complete general secretory pathway in gram-negative bacteria
30. Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli
31. Non-bilayer lipids are required for efficient protein transport across the plasma membrane of Escherichia coli
32. Increased unsaturated fatty acid production associated with a suppressor of the fabA6(Ts) mutation in Escherichia coli
33. Nucleotide binding activity of SecA homodimer is conformationally regulated by temperature and altered by prlD and azi mutations
34. - phenotype of a secB null mutant and the cold-sensitive phenotype of a secG null mutant
35. Direction of travel of RecBC recombinase through bacteriophage lambda DNA
36. Coupled structure changes of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and ΔsecG::kan double mutant
37. Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant
38. Biochemical characterization of the presecretory protein translocation machinery of Escherichia coli
39. Primary structures of the wild-type and mutant alleles encoding the phosphatidylglycerophosphate synthase of Escherichia coli
40. Role of lipids in the translocation of proteins across membranes
41. Proton motive force-dependent and -independent protein translocation revealed by an efficient in vitro assay system of Escherichia coli
42. In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP