Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli

BioTechniques

Volumn 58, Issue 2, 2015, Pages 86-88

  Miyazaki, Kentaro ^a,b  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

4 chloro phenylalanine; Counterselection; Escherichia coli; Phenylalanyl tRNA synthetase; PheS

Indexed keywords

4 CHLORO PHENYLALANINE; ALANINE; PHENYLALANINE DERIVATIVE; PHENYLALANINE TRANSFER RNA LIGASE; SERINE; UNCLASSIFIED DRUG; GENETIC MARKER; MUTANT PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CELL DEATH; COUNTER SELECTION; DNA SEQUENCE; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC PROCEDURES; GENETIC VARIABILITY; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; STRAIN IMPROVEMENT; BACTERIAL GENE; ENZYMOLOGY; FLUORESCENCE; GENETIC MARKER; GENETIC SELECTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PROCEDURES;

ESCHERICHIA COLI; LURIA;

ESCHERICHIA COLI; FLUORESCENCE; GENES, BACTERIAL; GENETIC ENGINEERING; GENETIC MARKERS; MUTANT PROTEINS; PHENYLALANINE-TRNA LIGASE; SELECTION, GENETIC;

EID: 84922715737     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114257     Document Type: Article

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