Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

PLoS ONE

Volumn 8, Issue 4, 2013, Pages

  Burke, Catherine ^a   Liu, Michael ^a   Britton, Warwick ^b,c   Triccas, James A ^b,c   Thomas, Torsten ^d   Smith, Adrian L ^b,c   Allen, Steven ^b,c   Salomon, Robert ^b,c   Harry, Elizabeth ^a  

^a UNIVERSITY OF TECHNOLOGY SYDNEY   (Australia)

^b CENTENARY INSTITUTE   (Australia)

^c UNIVERSITY OF SYDNEY   (Australia)

^d UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE BETA SUBUNIT;

AROB GENE; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CELL ASSAY; CELL CLONE; CELL CYCLE REGULATION; CELL DIVISION; CELL ISOLATION; CELL SELECTION; CELL STRUCTURE; CELL VIABILITY; COAE GENE; CONTROLLED STUDY; DAM GENE; DAMX GENE; DRUG TARGETING; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION; GENE IDENTIFICATION; GENE LIBRARY; GENE OVEREXPRESSION; HIGH THROUGHPUT SCREENING; HISA GENE; HISB GENE; HISH GENE; ILVC GENE; KIL GENE; LIGHT SCATTERING; MUTT GENE; NONHUMAN; PHENOTYPE; PPIA GENE; PPIC GENE; PROCESS DEVELOPMENT; RPOB GENE; THRW GENE; YACF GENE; YACG GENE; YAFW GENE; YCJY GENE; YCJZ GENE; YEJH GENE; YKFH GENE; YKFL GENE; YMFL GENE; YMFM GENE; YMFN GENE; YTFA GENE; YTFB GENE;

BACTERIAL PHYSIOLOGICAL PHENOMENA; ESCHERICHIA COLI; FLOW CYTOMETRY; GENES, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84875677063     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0060964     Document Type: Article

References (64)

1. Harry E, Monahan L, Thompson L, (2006) Bacterial cell division: the mechanism and its precison. Int Rev Cytol 253: 27-94.
2. Adams DW, Errington J, (2009) Bacterial cell division: assembly, maintenance and disassembly of the Z ring. Nat Rev Microbiol 7: 642-653.
3. de Boer PA, (2010) Advances in understanding E. coli cell fission. Curr Opin Microbiol 13: 730-737.
4. Lutkenhaus J, (1998) The regulation of bacterial cell division: a time and place for it. Curr Opin Microbiol 1: 210-215.
5. Ma S, Ma S, (2012) The Development of FtsZ Inhibitors as Potential Antibacterial Agents. ChemMedChem 7: 1161-1172.
6. Li ZR, Garner AL, Gloeckner C, Janda KD, Carlow CK (2011) Targeting the Wolbachia Cell Division Protein FtsZ as a New Approach for Antifilarial Therapy. Plos Neglected Tropical Diseases 5.
7. Lock RL, Harry EJ, (2008) Cell-division inhibitors: new insights for future antibiotics. Nat Rev Drug Discov 7: 324-338.
8. Stokes NR, Baker N, Bennett JM, Berry J, Collins I, et al. (2012) An Improved Small-Molecule Inhibitor of FtsZ with Superior In Vitro Potency, Drug-Like Properties and In Vivo Efficacy. Antimicrob Agents Chemother.
9. Vandeputte P, Vandillewijn J, Rorsch A, (1964) The Selection of Mutants of Escherichia-Coli with Impaired Cell Division at Elevated Temperature. Mutation Research 1: 121-128.
10. Margolin W, (2000) Themes and variations in prokaryotic cell division. Fems Microbiology Reviews 24: 531-548.
11. Wu LJ, Errington J, (2004) Coordination of cell division and chromosome segregation by a nucleoid occlusion protein in Bacillus subtilis. Cell 117: 915-925.
12. Bernhardt TG, de Boer PA, (2005) SlmA, a nucleoid-associated, FtsZ binding protein required for blocking septal ring assembly over Chromosomes in E. coli. Molecular Cell 18: 555-564.
13. de Boer PA, Crossley RE, Rothfield LI, (1988) Isolation and properties of minB, a complex genetic locus involved in correct placement of the division site in Escherichia coli. J Bacteriol 170: 2106-2112.
14. Rothfield L, Taghbalout A, Shih YL, (2005) Spatial control of bacterial division-site placement. Nat Rev Microbiol 3: 959-968.
15. Rodrigues CDA, Harry EJ, (2012) The Min System and Nucleoid Occlusion Are Not Required for Identifying the Division Site in Bacillus subtilis but Ensure Its Efficient Utilization. PLoS Genet 8: e1002561.
16. Chien AC, Hill NS, Levin PA, (2012) Cell size control in bacteria. Curr Biol 22: R340-349.
17. Thanbichler M, Shapiro L, (2006) MipZ, a spatial regulator coordinating chromosome segregation with cell division in Caulobacter. Cell 126: 147-162.
18. Treuner-Lange A, Aguiluz K, van der Does C, Gomez-Santos N, Harms A, et al. (2012) PomZ, a ParA-like protein, regulates Z-ring formation and cell division in Myxococcus xanthus. Mol Microbiol.
19. Willemse J, Borst JW, de Waal E, Bisseling T, van Wezel GP, (2011) Positive control of cell division: FtsZ is recruited by SsgB during sporulation of Streptomyces. Genes Dev 25: 89-99.
20. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Molecular Systems Biology 2.
21. Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, et al. (2003) Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683.
22. Sureka K, Hossain T, Mukherjee P, Chatterjee P, Datta P, et al. (2010) Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division. Plos One 5.
23. Yoon MY, Lee KM, Park Y, Yoon SS, (2011) Contribution of Cell Elongation to the Biofilm Formation of Pseudomonas aeruginosa during Anaerobic Respiration. Plos One 6.
24. Branda SS, Chu F, Kearns DB, Losick R, Kolter R, (2006) A major protein component of the Bacillus subtilis biofilm matrix. Mol Microbiol 59: 1229-1238.
25. Harshey RM, Matsuyama T, (1994) Dimorphic Transition in Escherichia-Coli and Salmonella-Typhimurium- Surface-Induced Differentiation into Hyperflagellate Swarmer Cells. Proc Natl Acad Sci U S A 91: 8631-8635.
26. Belas MR, Colwell RR, (1982) Scanning Electron-Microscope Observation of the Swarming Phenomenon of Vibrio-Parahaemolyticus. J Bacteriol 150: 956-959.
27. Pearson MM, Rasko DA, Smith SN, Mobley HLT, (2010) Transcriptome of Swarming Proteus mirabilis. Infection and Immunity 78: 2834-2845.
28. Ammendola A, Geisenberger O, Andersen JB, Givskov M, Schleifer KH, et al. (1998) Serratia liquefaciens swarm cells exhibit enhanced resistance to predation by Tetrahymena sp. Fems Microbiology Letters 164: 69-75.
29. Corno G, Jurgens K, (2006) Direct and indirect effects of protist predation on population size structure of a bacterial strain with high phenotypic plasticity. Applied and Environmental Microbiology 72: 78-86.
30. Chen K, Sun GW, Chua KL, Gan YH, (2005) Modified virulence of antibiotic-induced Burkholderia pseudomallei filaments. Antimicrobial Agents and Chemotherapy 49: 1002-1009.
31. Justice SS, Hunstad DA, Seed PC, Hultgren SJ, (2006) Filamentation by Escherichia coli subverts innate defenses during urinary tract infection. Proc Natl Acad Sci U S A 103: 19884-19889.
32. Saville SP, Lazzell AL, Bryant AP, Fretzen A, Monreal A, et al. (2006) Inhibition of filamentation can be used to treat disseminated candidiasis. Antimicrobial Agents and Chemotherapy 50: 3312-3316.
33. Draper GC, McLennan N, Begg K, Masters M, Donachie WD, (1998) Only the N-terminal domain of FtsK functions in cell division. J Bacteriol 180: 4621-4627.
34. Ward JE Jr, Lutkenhaus J, (1985) Overproduction of FtsZ induces minicell formation in E. coli. Cell 42: 941-949.
35. de Oliveira IFF, Borges AD, Kooij V, Bartosiak-Jentys J, Luirink J, et al. (2010) Characterization of ftsZ Mutations that Render Bacillus subtilis Resistant to MinC. Plos One 5.
36. Dai K, Lutkenhaus J, (1992) The Proper Ratio of Ftsz to Ftsa Is Required for Cell-Division to Occur in Escherichia-Coli. J Bacteriol 174: 6145-6151.
37. Deboer PAJ, Crossley RE, Rothfield LI, (1992) Roles of Minc and Mind in the Site-Specific Septation Block Mediated by the Mincde System of Escherichia-Coli. J Bacteriol 174: 63-70.
38. Camberg JL, Hoskins JR, Wickner S, (2009) ClpXP protease degrades the cytoskeletal protein, FtsZ, and modulates FtsZ polymer dynamics. Proc Natl Acad Sci U S A 106: 10614-10619.
39. Huisman O, Dari R, Gottesman S, (1984) Cell-Division Control in Escherichia coli- Specific Induction of the SOS Function sfiA Protein Is Sufficient to Block Septation. Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences 81: 4490-4494.
40. England K, Crew R, Slayden RA, (2011) Mycobacterium tuberculosis septum site determining protein, Ssd encoded by rv3660c, promotes filamentation and elicits an alternative metabolic and dormancy stress response. Bmc Microbiology 11.
41. Modell JW, Hopkins AC, Laub MT, (2011) A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsW. Genes & Development 25: 1328-1343.
42. Resina-Pelfort O, Comas-Riu J, Vives-Rego J, (2001) Effects of deflected droplet electrostatic cell sorting on the viability and exoproteolytic activity of bacterial cultures and marine bacterioplankton. Systematic and Applied Microbiology 24: 31-36.
43. Pogliano J, Pogliano K, Weiss DS, Losick R, Beckwith J, (1997) Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sites. Proc Natl Acad Sci U S A 94: 559-564.
44. Guzman LM, Belin D, Carson MJ, Beckwith J, (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177: 4121-4130.
45. Dai K, Lutkenhaus J, (1991) ftsZ is an essential cell division gene in Escherichia coli. J Bacteriol 173: 3500-3506.
46. Lauder SD, Kowalczykowski SC, (1993) Negative co-dominant inhibition of RecA protein function. Biochemical properties of the recA1, recA13 and recA56 proteins and the effect of recA56 protein on the activities of the wild-type recA protein function in vitro. J Mol Biol 234: 72-86.
47. Mellies JL, Haack KR, Galligan DC, (2007) SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. J Bacteriol 189: 2863-2872.
48. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, et al. (1997) The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462.
49. Hoffman RA (2009) Pulse width for particle sizing. In: Robinson JP, editor. Current Protocols in Flow Cytometry: Wiley Interscience pp. 1.23.21-21.23.17.
50. van Dijk MA, Gregori G, Hoogveld HL, Rijkeboer M, Denis M, et al. (2010) Optimizing the setup of a flow cytometric cell sorter for efficient quantitative sorting of long filamentous cyanobacteria. Cytometry A 77: 911-924.
51. Becton DC (2003) BD FACSAria User's Guide. In: Becton DaC, editor.
52. Siegele DA, Hu JC, (1997) Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci U S A 94: 8168-8172.
53. Khlebnikov A, Risa O, Skaug T, Carrier TA, Keasling JD, (2000) Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture. J Bacteriol 182: 7029-7034.
54. Lyngstadaas A, Lobner-Olesen A, Boye E, (1995) Characterization of three genes in the dam-containing operon of Escherichia coli. Mol Gen Genet 247: 546-554.
55. Arends SJR, Williams K, Scott RJ, Rolong S, Popham DL, et al. (2010) Discovery and Characterization of Three New Escherichia coli Septal Ring Proteins That Contain a SPOR Domain: DamX, DedD, and RlpA. J Bacteriol 192: 242-255.
56. Lopez-Garrido J, Casadesus J, (2010) The DamX protein of Escherichia coli and Salmonella enterica. Gut Microbes 1: 285-288.
57. Frandsen N, D'Ari R, (1993) Excess histidine enzymes cause AICAR-independent filamentation in Escherichia coli. Mol Gen Genet 240: 348-354.
58. Conter A, Bouche JP, Dassain M, (1996) Identification of a new inhibitor of essential division gene ftsZ as the kil gene of defective prophage Rac. J Bacteriol 178: 5100-5104.
59. Mehta P, Casjens S, Krishnaswamy S (2004) Analysis of the lambdoid prophage element e14 in the E-coli K-12 genome. Bmc Microbiology 4.
60. Plasterk RHA, Vandeputte P, (1985) The Invertible P-DNA Segment in the Chromosome of Escherichia-Coli. Embo Journal 4: 237-242.
61. Maguin E, Brody H, Hill CW, Dari R, (1986) Sos-Associated Division Inhibition Gene Sfic Is Part of Excisable Element E14 in Escherichia-Coli. J Bacteriol 168: 464-466.
62. Uehara T, Bernhardt TG, (2011) More than just lysins: peptidoglycan hydrolases tailor the cell wall. Curr Opin Microbiol 14: 698-703.
63. Wyckoff TJ, Taylor JA, Salama NR, (2012) Beyond growth: novel functions for bacterial cell wall hydrolases. Trends Microbiol 20: 540-547.
64. Sambrook J, Russell D (2001) Molecular Cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press.