Reprogramming of human fibroblasts toward a cardiac fate

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 14, 2013, Pages 5588-5593

  Nam, Young Jae ^a   Song, Kunhua ^a   Luo, Xiang ^a   Daniel, Edward ^a   Lambeth, Kaleb ^a   West, Katherine ^a   Hill, Joseph A ^a   Di Maio, J Michael ^a   Baker, Linda A ^a   Bassel Duby, Rhonda ^a   Olson, Eric N ^a  

^a UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Cardiogenesis; Cell fate specification; Phenotypic switching; Regeneration

Indexed keywords

BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR; BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR HAND2; CALCIUM; GATA BINDING PROTEIN 4; MICRORNA; MICRORNA 1; MICRORNA 133; MYOCARDIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR T BOX5; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CARDIAC FATE; CELL FATE; CONTROLLED STUDY; FIBROBLAST; GENE; GENE EXPRESSION; GENE REPRESSION; HEART; HEART MUSCLE CELL; HEART MUSCLE CONTRACTILITY; HUMAN; HUMAN CELL; HUMAN TISSUE; NEWBORN; PHENOTYPE; PRIORITY JOURNAL; SARCOMERE;

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CELL TRANSDIFFERENTIATION; FIBROBLASTS; FLOW CYTOMETRY; GATA4 TRANSCRIPTION FACTOR; GENE EXPRESSION REGULATION; HUMANS; IMMUNOHISTOCHEMISTRY; MICRORNAS; MYOCYTES, CARDIAC; PHENOTYPE; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84875848994     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1301019110     Document Type: Article

References (36)

1. Graf T, Enver T (2009) Forcing cells to change lineages. Nature 462(7273):587-594.
2. Vierbuchen T, Wernig M (2011) Direct lineage conversions: Unnatural but useful? Nat Biotechnol 29(10):892-907.
3. Davis RL, Weintraub H, Lassar AB (1987) Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell 51(6):987-1000.
4. Olson EN (1990) MyoD family: A paradigm for development? Genes Dev 4(9):1454-1461.
5. Wang D, et al. (2001) Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor. Cell 105(7):851-862.
6. Wang Z, Wang DZ, Pipes GC, Olson EN (2003) Myocardin is a master regulator of smooth muscle gene expression. Proc Natl Acad Sci USA 100(12):7129-7134.
7. Chen J, Kitchen CM, Streb JW, Miano JM (2002) Myocardin: A component of a molecular switch for smooth muscle differentiation. J Mol Cell Cardiol 34(10):1345-1356.
8. Du KL, et al. (2003) Myocardin is a critical serum response factor cofactor in the transcriptional program regulating smooth muscle cell differentiation. Mol Cell Biol 23(7):2425-2437.
9. Yoshida T, et al. (2003) Myocardin is a key regulator of CArG-dependent transcription of multiple smooth muscle marker genes. Circ Res 92(8):856-864.
10. Ieda M, et al. (2010) Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 142(3):375-386.
11. Song K, et al. (2012) Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature 485(7400):599-604.
12. Qian L, et al. (2012) In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature 485(7400):593-598.
13. Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA (2008) In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Nature 455(7213):627-632.
14. Huang P, et al. (2011) Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors. Nature 475(7356):386-389.
15. Sekiya S, Suzuki A (2011) Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475(7356):390-393.
16. Pang ZP, et al. (2011) Induction of human neuronal cells by defined transcription factors. Nature 476(7359):220-223.
17. Qiang L, et al. (2011) Directed conversion of Alzheimer's disease patient skin fibroblasts into functional neurons. Cell 146(3):359-371.
18. Yoo AS, et al. (2011) MicroRNA-mediated conversion of human fibroblasts to neurons. Nature 476(7359):228-231.
19. Jayawardena TM, et al. (2012) MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes. Circ Res 110(11):1465-1473.
20. Kuro-o M, et al. (1989) Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. J Biol Chem 264(31):18272-18275.
21. Rovner AS, Thompson MM, Murphy RA (1986) Two different heavy chains are found in smooth muscle myosin. Am J Physiol 250(6 Pt 1):C861-C870.
22. Owens GK (1995) Regulation of differentiation of vascular smooth muscle cells. Physiol Rev 75(3):487-517.
23. Liu N, et al. (2008) microRNA-133a regulates cardiomyocyte proliferation and suppresses smooth muscle gene expression in the heart. Genes Dev 22(23):3242-3254.
24. Brown RD, Ambler SK, Mitchell MD, Long CS (2005) The cardiac fibroblast: Therapeutic target in myocardial remodeling and failure. Annu Rev Pharmacol Toxicol 45: 657-687.
25. Zeisberg EM, Kalluri R (2010) Origins of cardiac fibroblasts. Circ Res 107(11):1304-1312.
26. Dimmeler S, Zeiher AM, Schneider MD (2005) Unchain my heart: The scientific foundations of cardiac repair. J Clin Invest 115(3):572-583.
27. Srivastava D, Ieda M(2012) Critical factors for cardiac reprogramming. Circ Res 111(1):5-8.
28. Protze S, et al. (2012) A new approach to transcription factor screening for reprogramming of fibroblasts to cardiomyocyte-like cells. J Mol Cell Cardiol 53(3):323-332.
29. Li S, Wang DZ, Wang Z, Richardson JA, Olson EN (2003) The serum response factor coactivator myocardin is required for vascular smooth muscle development. Proc Natl Acad Sci USA 100(16):9366-9370.
30. Ghosh TK, et al. (2009) Physical interaction between TBX5 and MEF2C is required for early heart development. Mol Cell Biol 29(8):2205-2218.
31. Wang C, Cao D, Wang Q, Wang DZ (2011) Synergistic activation of cardiac genes by myocardin and Tbx5. PLoS ONE 6(8):e24242.
32. Ivey KN, et al. (2008) MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. Cell Stem Cell 2(3):219-229.
33. Zhou Q, Melton DA (2008) Extreme makeover: Converting one cell into another. Cell Stem Cell 3(4):382-388.
34. Islas JF, et al. (2012) Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors. Proc Natl Acad Sci USA 109(32): 13016-13021.
35. Zwi L, et al. (2009) Cardiomyocyte differentiation of human induced pluripotent stem cells. Circulation 120(15):1513-1523.
36. Shiba Y, et al. (2012) Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts. Nature 489(7415):322-325.