Native Immunogold Labeling of Cell Surface Proteins and Viral Glycoproteins for Cryo-Electron Microscopy and Cryo-Electron Tomography Applications

Journal of Histochemistry and Cytochemistry

Volumn 63, Issue 10, 2015, Pages 780-792

  Yi, Hong ^a   Strauss, Joshua D ^b   Ke, Zunlong ^c   Alonas, Eric ^d   Dillard, Rebecca S ^b   Hampton, Cheri M ^b   Lamb, Kristen M ^b   Hammonds, Jason E ^b   Santangelo, Philip J ^d   Spearman, Paul W ^b   Wright, Elizabeth R ^a,b  

^a EMORY UNIVERSITY   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^d Emory University   (United States)

Author keywords

cryo electron microscopy (cryo EM); cryo electron tomography (cryo ET); immuno electron microscopy (immuno EM); transmission electron microscopy (TEM)

Indexed keywords

CELL SURFACE PROTEIN; VIRUS GLYCOPROTEIN; GLYCOPROTEIN; MEMBRANE PROTEIN; VIRAL PROTEIN;

ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CULTURE MEDIUM; ELECTRON TOMOGRAPHY; FEMALE; HELA CELL LINE; HEP 2 CELL LINE; HUMAN; HUMAN CELL; HUMAN RESPIRATORY SYNCYTIAL VIRUS; IMAGE PROCESSING; IMMUNOGOLD LABELING; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE; VIRUS ASSEMBLY; VIRUS INFECTION; VIRUS PARTICLE; VIRUS PURIFICATION; VIRUS RELEASE; VITRIFICATION; ANIMAL; CELL LINE; CHEMISTRY; IMMUNOHISTOCHEMISTRY; PROCEDURES; RESPIRATORY SYNCYTIAL VIRUS INFECTION; ULTRASTRUCTURE; VIROLOGY;

ANIMALS; CELL LINE; CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPE TOMOGRAPHY; GLYCOPROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; MEMBRANE PROTEINS; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUSES; VIRAL PROTEINS;

EID: 84942435656     PISSN: 00221554     EISSN: 15515044     Source Type: Journal    
DOI: 10.1369/0022155415593323     Document Type: Article

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