Metallothioneins for correlative light and electron microscopy

Methods in Cell Biology

Volumn 124, Issue , 2014, Pages 55-70

  Fernández de Castro, Isabel ^a   Sanz Sánchez, Laura ^a   Risco, Cristina ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

CLEM; Clonable tags for TEM; Correlative light and electron microscopy; Electron tomography; GFP; Immunogold; Metal tagging TEM; Metallothionein; METTEM; Molecular mapping

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; METALLOTHIONEIN;

ANIMAL; BIOSYNTHESIS; CELL LINE; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; HUMAN; PROCEDURES; STAINING; TRANSMISSION ELECTRON MICROSCOPY; ULTRAMICROTOMY; ULTRASTRUCTURE; YEAST;

ANIMALS; CELL LINE; CRYOULTRAMICROTOMY; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HUMANS; METALLOTHIONEIN; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING; YEASTS;

EID: 84907846407     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801075-4.00003-3     Document Type: Chapter

References (28)

1. Ariyasu S., Onoda A., Sakamoto R., Yamamura T. Alignment of gold clusters on DNA via a DNA-recognizing zinc finger-metallothionein fusion protein. Bioconjugate Chemistry 2009, 20:2278-2285.
2. Barajas D., Fernández de Castro I., Pogany J., Risco C., Nagy P.D. Noncanonical role for the host Vps4 AAA+ ATPase ESCRT protein in the formation of the Tomato bushy stunt virus replicase. PLoS Pathogens 2014, 10:e1004087.
3. Beyersmann D., Haase H. Functions of zinc in signaling, proliferation and differentiation of mammalian cells. Biometals 2001, 14:331-341.
4. Bouchet-Marquis C., Pagratis M., Kirmse R., Hoenger A. Metallothionein as a clonable high-density marker for cryo-electron microscopy. Journal of Structural Biology 2012, 177:119-127.
5. Chan J., Huang Z., Merrifield M.E., Salgado M.T., Stillman M.J. Studies of metal binding reactions in metallothioneins by spectroscopic, molecular biology, and molecular modelling techniques. Coordination Chemistry Reviews 2002, 233-234:319-339.
6. Delebecque C.J., Lindner A.B., Silver P.A., Aldaye F.A. Organization of intracellular reactions with rationally designed RNA assemblies. Science 2011, 333:470-474.
7. Diestra E., Cayrol B., Arluison V., Risco C. Cellular electron microscopy imaging reveals membrane localization of the bacterial Hfq protein. PLoS One 2009, 4:e8301.
8. Diestra E., Fontana J., Guichard P., Marco S., Risco C. Visualization of proteins in intact cells with a clonable tag for electron microscopy. Journal of Structural Biology 2009, 165:157-168.
9. Fernández de Castro I., Zamora P.F., Ooms L., Fernández J.J., Lai C.M.-H., Mainou B.A., et al. Reovirus forms neo-organelles for progeny particle assembly within reorganized cell membranes. mBio 2014, 5:e00931-13.
10. Fontana J., López-Iglesias C., Tzeng W.-P., Frey T.K., Fernández J.J., Risco C. Three-dimensional structure of Rubella virus factories. Virology 2010, 405:579-591.
11. Fontana J., López-Montero N., Elliott R.M., Fernández J.J., Risco C. The unique architecture of Bunyamwera virus factories around the Golgi complex. Cellular Microbiology 2008, 10:2012-2028.
12. Fontana J., Tzeng W.-P., Calderita G., Fraile-Ramos A., Frey T.K., Risco C. Novel replication complex architecture in rubella replicon-transfected cells. Cellular Microbiology 2007, 9:875-890.
13. Fukunaga Y., Higashihara A., Nishino Y., Yasunaga T., Jin M., Miyazawa A. Enhanced detection efficiency of genetically encoded tag allows the visualization of monomeric proteins by electron microscopy. Journal of Electron Microscopy 2012, 61:229-236.
14. Fukunaga Y., Hirase A., Kim H., Wada N., Nishino Y., Miyazawa A. Electron microscopic analysis of a fusion protein of postsynaptic density-95 and metallothionein in cultured hippocampal neurons. Journal of Electron Microscopy 2007, 56:119-129.
15. Furey W.F., Robbins A.H., Clancy L.L., Winge D.R., Wang B.C., Stout C.D. Crystal structure of Cd, Zn metallothionein. Science 1986, 23:704-710.
16. Hurbain I., Sachse M. The future is cold: Cryo-preparation methods for transmission electron microscopy of cells. Biology of the Cell 2011, 103:405-420.
17. Kukulski W., Schorb M., Welsch S., Picco A., Kaksonen M., Briggs J.A. Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. Journal of Cell Biology 2011, 192:111-119.
18. Mercogliano C.P., DeRosier D.J. Gold nanocluster formation using metallothionein: Mass spectrometry and electron microscopy. Journal of Molecular Biology 2006, 355:211-223.
19. Mercogliano C.P., DeRosier D.J. Concatenated metallothionein as a clonable gold label for electron microscopy. Journal of Structural Biology 2007, 160:70-82.
20. Nielson K.B., Atkin C.L., Winge D.R. Distinct metal-binding configurations in metallothionein. Journal of Biological Chemistry 1985, 260:5342-5350.
21. Rae T.D., Schmidt P.J., Pufahl R.A., Culotta V.C., O'Halloran T.V. Undetectable intracellular free copper: The requirement of a copper chaperone for superoxide dismutase. Science 1999, 284:805-808.
22. Risco C., Sanmartín-Conesa E., Tzeng W.-P., Frey T.K., Seybold V., de Groot R.J. Specific, sensitive, high-resolution detection of protein molecules in eukaryotic cells using metal-tagging transmission electron microscopy. Structure 2012, 20:759-766.
23. Sanz-Sánchez L., Risco C. Multilamellar structures and filament bundles are found on the cell surface during bunyavirus egress. PLoS One 2013, 8:e65526.
24. Schmitz G., Minkel D.T., Gingrich D., Shaw C.F. The binding of Gold(I) to metallothionein. Journal of Inorganic Biochemistry 1980, 112:293-306.
25. Shi X., Kohl A., Leonard V.H., Li P., McLees A., Elliott R.M. Requirement of the N-terminal region of orthobunyavirus nonstructural protein NSm for virus assembly and morphogenesis. Journal of Virology 2006, 80:8089-8099.
26. Shu X., Lev-Ram V., Deerinck T.J., Qi Y., Ramko E.B., Davidson M.W., et al. A genetically encoded tag for correlated light and electron microscopy of intact cells. PLoS Biology 2011, 9:e10011041.
27. Slot J.W., Geuze H.J. Cryosectioning and immunolabeling. Nature Protocols 2007, 2:2480-2491.
28. Zhou Q., Sun S., Tai P., Sui S.-F. Structural characterization of the complex of SecB and metallothionein-labeled proOmpA by cryo-electron microscopy. PLoS One 2012, 7:e47015.