Visualizing active membrane protein complexes by electron cryotomography

Nature Communications

Volumn 5, Issue , 2014, Pages

  Gold, Vicki A M ^a   Ieva, Raffaele ^b   Walter, Andreas ^a   Pfanner, Nikolaus ^b   Van Der Laan, Martin ^b   Kühlbrandt, Werner ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; MEMBRANE PROTEIN; MITOCHONDRIAL PROTEIN; PROTEIN PRECURSOR; QUANTUM DOT; SACCHAROMYCES CEREVISIAE PROTEIN;

ELECTRON; LIPID; MEMBRANE; MITOCHONDRION; PROTEIN; TOMOGRAPHY; VISUALIZATION;

ANALYTIC METHOD; ARTICLE; BIOTINYLATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ELECTRON; ELECTRON CRYOTOMOGRAPHY; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; FUNGAL STRAIN; IN VITRO STUDY; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SPECIFICALLY TARGETED MEMBRANE NANOPARTICLE TECHNIQUE; CHEMISTRY; CRYOELECTRON MICROSCOPY; EVALUATION STUDY; METABOLISM; PROCEDURES; PROTEIN TRANSPORT; ULTRASTRUCTURE;

CRYOELECTRON MICROSCOPY; MEMBRANE PROTEINS; MITOCHONDRIA; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84903121701     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms5129     Document Type: Article

References (39)

1. Wang, Q., Mercogliano, C. P. & Löwe, J. A ferritin-based label for cellular electron cryotomography. Structure 19, 147-154 (2011).
2. Mercogliano, C. P. & DeRosier, D. J. Concatenated metallothionein as a clonable gold label for electron microscopy. J. Struct. Biol. 160, 70-82 (2007). (Pubitemid 47405236)
3. Harrison, P. M. & Arosio, P. The ferritins: molecular properties, iron storage function and cellular regulation. Biochim. Biophys. Acta 1275, 161-203 (1996). (Pubitemid 26248989)
4. Mercogliano, C. P. & DeRosier, D. J. Gold nanocluster formation using metallothionein: mass spectrometry and electron microscopy. J. Mol. Biol. 355, 211-223 (2006). (Pubitemid 41774126)
5. Risco, C. et al. Specific, sensitive, high-resolution detection of protein molecules in eukaryotic cells using metal-tagging transmission electron microscopy. Structure 20, 759-766 (2012).
6. Diestra, E., Fontana, J., Guichard, P., Marco, S. & Risco, C. Visualization of proteins in intact cells with a clonable tag for electron microscopy. J. Struct. Biol. 165, 157-168 (2009).
7. Müller-Reichert, T. & Verkade, P. Correlative light and electron microscopy. Methods Cell Biol. 111 (2012).
8. Hoffmann, C. et al. Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat. Protoc. 5, 1666-1677 (2010).
9. Gaietta, G. M. et al. Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc. Natl Acad. Sci. USA 103, 17777-17782 (2006). (Pubitemid 44852236)
10. Deerinck, J. The application of fluorescent quantum dots to confocal, multiphoton, and electron microscopic imaging. Toxicol. Pathol. 36, 112-116 (2008).
11. Nisman, R., Dellaire, G., Ren, Y., Li, R. & Bazett-Jones, D. P. Application of quantum dots as probes for correlative fluorescence, conventional, and energyfiltered transmission electron microscopy. J. Histochem. Cytochem. 52, 13-18 (2004). (Pubitemid 38032129)
12. Giepmans, B. N., Deerinck, T. J., Smarr, B. L., Jones, Y. Z. & Ellisman, M. H. Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots. Nat. Methods 2, 743-749 (2005). (Pubitemid 41486205)
13. Mokranjac, D. & Neupert, W. The many faces of the mitochondrial TIM23 complex. Biochim. Biophys. Acta 1797, 1045-1054 (2010).
14. Schmidt, O., Pfanner, N. & Meisinger, C. Mitochondrial protein import: from proteomics to functional mechanisms. Nat. Rev. Mol. Cell Biol. 11, 655-667 (2010).
15. Endo, T. & Yamano, K. Multiple pathways for mitochondrial protein traffic. Biol. Chem. 390, 723-730 (2009).
16. Rassow, J. et al. Translocation arrest by reversible folding of a precursor protein imported into mitochondria-a means to quantitate translocation contact sites. J. Cell Biol. 109, 1421-1428 (1989). (Pubitemid 19251200)
17. Reichert, A. S. & Neupert, W. Contact sites between the outer and inner membrane of mitochondria-role in protein transport. Biochim. Biophys. Acta 1592, 41-49 (2002). (Pubitemid 35015164)
18. Dekker, P. J. et al. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44. EMBO J. 16, 5408-5419 (1997). (Pubitemid 27380148)
19. Mannella, C. A. Structure and dynamics of the mitochondrial inner membrane cristae. Biochim. Biophys. Acta 1763, 542-548 (2006). (Pubitemid 44015951)
20. Zick, M., Rabl, R. & Reichert, A. S. Cristae formation-linking ultrastructure and function of mitochondria. Biochim. Biophys. Acta 1793, 5-19 (2009).
21. Frank, J. Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell (Springer Publishing, 2008).
22. Meeusen, S., McCaffery, J. M. & Nunnari, J. Mitochondrial fusion intermediates revealed in vitro. Science 305, 1747-1752 (2004). (Pubitemid 39249636)
23. Wurm, C. A. et al. Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient. Proc. Natl Acad. Sci. 108, 13546-13551 (2011).
24. van der Laan, M., Bohnert, M., Wiedemann, N. & Pfanner, N. Role of MINOS in mitochondrial membrane architecture and biogenesis. Trends Cell Biol. 22, 185-192 (2012).
25. Vogel, F., Bornhövd, C., Neupert, W. & Reichert, A. S. Dynamic subcompartmentalization of the mitochondrial inner membrane. J. Cell Biol. 175, 237-247 (2006). (Pubitemid 44631418)
26. Chacinska, A. et al. Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-829 (2005). (Pubitemid 40568799)
27. Tamura, Y. et al. Tim23-Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import. J. Cell Biol. 184, 129-141 (2009).
28. Wu, Y. & Sha, B. Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p. Nat. Struct. Mol. Biol. 13, 589-593 (2006). (Pubitemid 44036468)
29. Beckett, D., Kovaleva, E. & Schatz, P. J. A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation. Prot. Sci. 8, 921-929 (1999). (Pubitemid 29165423)
30. Lau, P. W., Potter, C. S., Carragher, B. & MacRae, I. J. DOLORS: versatile strategy for internal labeling and domain localization in electron microscopy. Structure. 20, 1995-2002 (2012).
31. Dubavik, A. et al. Penetration of amphiphilic quantum dots through model and cellular plasma membranes. ACS Nano 6, 2150-2156 (2012).
32. Koll, H. et al. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell 68, 1163-1175 (1992).
33. Sikorski, R., S. & Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27 (1989).
34. Meisinger, C., Pfanner, N. & Truscott, K. N. Isolation of yeast mitochondria. Methods Mol. Biol. 313, 33-39 (2006).
35. Gebert, M. et al. Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J. Cell Biol. 197, 595-604 (2012).
36. Kremer, J. R., Mastronade, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71-76 (1996). (Pubitemid 26093126)
37. Frangakis, A. S. & Hegerl, R. Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion. J. Struct. Biol. 135, 239-250 (2001).
38. Thomsen, K. Surface Area of an Ellipsoid www.numericana.com (2004).
39. Nicastro, D. et al. The molecular architecture of axonemes revealed by cryoelectron tomography. Science 313, 944-948 (2006). (Pubitemid 44267380)