Gene Targeting in Embryonic Stem Cells: II. Conditional Technologies

Transgenic Animal Technology: A Laboratory Handbook: Second Edition

Volumn , Issue , 2002, Pages 143-171

  Rucker, Edmund B ^a   Thomson, James G ^b   Piedrahita, Jorge A ^c  

^a UNIVERSITY OF MISSOURI   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84941109816     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-0-08-057480-6.50009-5     Document Type: Chapter

References (114)

1. Abremski K.E., Hoess R.H. Evidence for a second conserved arginine residue in the integrase family of recombination proteins. Protein Eng. 1992, 5:87-91.
2. Abremski K.E., Hoess R.H., Sternberg N. Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination. Cell 1983, 32:1301-1311.
3. Abremski K., Frommer B., Wierzbicki A., Hoess R.H. Properties of a mutant Cre protein that alters the topological linkage of recombination products. J. Mol. Biol. 1988, 202:59-66.
4. Abuin A., Bradley A. Recycling selectable markers in mouse embryonic stem cells. Mol. Cell. Biol. 1996, 16:1851-1856.
5. Abuin A., Zhang H., Bradley A. Genetic analysis of mouse embryonic stem cells bearing Msh3 and Msh2 single and compound mutations. Mol. Cell. Biol. 2000, 20:149-157.
6. Albert H., Dale E.C., Lee E., Ow D.W. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 1995, 7:649-659.
7. Andrews B.J., Proteau G.A., Beatty L.G., Sadowski P.D. The FLP recombinase of the 2 micron circle DNA of yeast: interaction with its target sequences. Cell 1985, 40:795-803.
8. Araki K., Araki M., Miyazaki J., Vassalli P. Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase. Proc. Natl. Acad. Sci. USA 1995, 92:160-164.
9. Araki K., Araki M., Yamamura K. Targeted integration of DNA using mutant lox sites in embryonic stem cells. Nucleic Acids Res. 1997, 25:868-872.
10. Askew G.R., Doetschman T., Lingrel J.B. Site-directed point mutations in embryonic stem cells: a gene-targeting tag-and-exchange strategy. Mol. Cell. Biol. 1993, 13:4115-4124.
11. Austin S., Ziese M., Sternberg N. A novel role for site-specific recombination in maintenance of bacterial replicons. Cell 1981, 25:729-736.
12. Baubonis W., Sauer B. Genomic targeting with purified Cre recombinase. Nucleic Acids Res. 1993, 21:2025-2029.
13. Bayley C.C., Morgan M., Dale E.C., Ow D.W. Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Mol. Biol. 1992, 18:353-361.
14. Behringer R.R., Ryan T.M., Reilly M.P., Asakura T., Palmiter R.D., Brinster R.L., Townes T.M. Synthesis of functional human hemoglobin in transgenic mice. Science 1989, 245:971-973.
15. Bethke B., Sauer B. Segmental genomic replacement by Cre-mediated recombination: genotoxic stress activation of the p53 promoter in single-copy transformants. Nucleic Acids Res. 1997, 25:2828-2834.
16. Brinster R.L., Allen J.M., Behringer R.R., Gelinas R.E., Palmiter R.D. Introns increase transcriptional efficiency in transgenic mice. Proc. Natl. Acad. Sci. USA 1988, 85:836-840.
17. Bunting M., Bernstein K.E., Greer J.M., Capecchi M.R., Thomas K.R. Targeting genes for self-excision in the germ line. Genes Dev. 1999, 13:1524-1528.
18. Call L.M., Moore O.S., Stetten G., Gearhart J.D. A Cre-lox recombination system for the targeted integration of circular yeast artificial chromosomes into embryonic stem cells. Hum. Mol. Genet. 2000, 9:1745-1751.
19. Chao C., Saito S., Kang J., Anderson C.W., Appella E., Xu Y. p53 transcriptional activity is essential for p53-dependent apoptosis following DNA damage. EMBO J. 2000, 19:4967-4975.
20. Choi S., Begum D., Koshinsky H., Ow D.W., Wing R.A. A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination. Nucleic Acids Res. 2000, 28:E19.
21. Cronin C.A., Gluba W., Scrable H. The lac operator-repressor system is functional in the mouse. Genes Dev. 2001, 15:1506-1517.
22. Dale E.C., Ow D.W. Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 1991, 88:10558-10562.
23. Day C.D., Lee E., Kobayashi J., Holappa L.D., Albert H., Ow D.W. Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced. Genes Dev. 2000, 14:2869-2880.
24. Deng C., Capecchi M.R. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol. Cell. Biol. 1992, 12:3365-3371.
25. DiSanto J.P., Muller W., Guy-Grand D., Fischer A., Rajewsky K. Lymphoid development in mice with a targeted deletion of the interleukin 2 receptor gamma chain. Proc. Natl. Acad. Sci. USA 1995, 92:377-381.
26. Doetschman T., Gregg R.G., Maeda N., Hooper M.L., Melton D.W., Thompson S., Smithies O. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 1987, 330:576-578.
27. Doetschman T., Maeda N., Smithies O. Targeted mutation of the Hprt gene in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 1988, 85:8583-8587.
28. Evans M.J., Kaufman M.H. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981, 292:154-156.
29. Forlino A., Porter F.D., Lee E.J., Westphal H., Marini J.C. Use of the Cre/lox recombination system to develop a non-lethal knock-in murine model for osteogenesis imperfecta with an alpha1(I) G349C substitution. Variability in phenotype in BrtllV mice. J. Biol. Chem. 1999, 274:37923-37931.
30. Furth P.A., St.Onge L., Boger H., Gruss P., Gossen M., Kistner A., Bujard H., Hennighausen L. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc. Natl. Acad. Sci. USA 1994, 91:9302-9306.
31. Gagneten S., Le Y., Miller J., Sauer B. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Nucleic Acids Res. 1997, 25:3326-3331.
32. Gordon J.W., Ruddle F.H. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science 1981, 214:1244-1246.
33. Gordon J.W., Scangos G.A., Plotkin D.J., Barbosa J.A., Ruddle F.H. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA 1980, 77:7380-7384.
34. Harada N., Tamai Y., Ishikawa T., Sauer B., Takaku K., Oshima M., Taketo M.M. Intestinal polyposis in mice with a dominant stable mutation of the beta-catenin gene. EMBO J. 1999, 18:5931-5942.
35. Harvey M., Vogel H., Morris D., Bradley A., Bernstein A., Donehower L.A. A mutant p53 transgene accelerates tumour development in heterozygous but not nullizygous p53-deficient mice. Nature Genet. 1995, 9:305-311.
36. Hasty P., Ramirez-Solis R., Krumlauf R., Bradley A. Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells. Nature 1991, 350:243-246.
37. Hasty P., Rivera-Perez J., Chang C., Bradley A. Target frequency and integration pattern for insertion and replacement vectors in embryonic stem cells. Mol. Cell. Biol. 1991, 11:4509-4517.
38. Hasty P., Crist M., Grompe M., Bradley A. Efficiency of insertion versus replacement vector targeting varies at different chromosomal loci. Mol. Cell. Biol. 1994, 14:8385-8390.
39. Hennet T., Hagen F.K., Tabak L.A., Marth J.D. T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination. Proc. Natl. Acad. Sci. USA 1995, 92:12070-12074.
40. Hirasawa M., Cho A., Sreenath T., Sauer B., Julien J., Kulkarni A.B. Neuron-specific expression of Cre recombinase during the late phase of brain development. Neurosci. Res. 2001, 40:125-132.
41. Hoess R.H., Abremski K. Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system. J. Mol. Biol. 1985, 181:351-362.
42. Hoess R.H., Ziese M., Sternberg N. P1 site-specific recombination: nucleotide sequence of the recombining sites. Proc. Natl. Acad. Sci. USA 1982, 79:3398-3402.
43. Hoess R.H., Wierzbicki A., Abremski K. The role of the loxP spacer region in P1 site-specific recombination. Nucleic Acids Res. 1986, 14:2287-2300.
44. Hoess R., Abremski K., Irwin S., Kendall M., Mack A. DNA specificity of the Cre recombinase resides in the 25-kDa carboxyl domain of the protein. J. Mol. Biol. 1990, 216:873-882.
45. Jacenko O., LuValle P., Solum K., Olsen B.R. A dominant negative mutation in the alpha 1(X) collagen gene produces spondylometaphyseal defects in mice. Prog. Clin. Biol. Res. 1993, 383B:427-436.
46. Jankowsky J.L., Slunt H.H., Ratovitski T., Jenkins N.A., Copeland N.G., Borchelt D.R. Co-expression of multiple transgenes in mouse CNS: a comparison of strategies. Biomol. Eng. 2001, 17:157-165.
47. Justice M.J., Zheng B., Woychik R.P., Bradley A. Using targeted large deletions and high-efficiency N-ethyl-N-nitrosourea mutagenesis for functional analyses of the mammalian genome. Methods 1997, 13:423-436.
48. Kanegae Y., Lee G., Sato Y., Tanaka M., Nakai M., Sakaki T., Sugano S., Saito I. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 1995, 23:3816-3821.
49. Kellendonk C., Tronche F., Monaghan A.P., Angrand P.O., Stewart F., Schutz G. Regulation of Cre recombinase activity by the synthetic steroid RU486. Nucleic Acids Res. 1996, 24:1404-1411.
50. Kitayama K., Abe M., Kakizaki T., Honma D., Natsume R., Fukaya M., Watanabe M., Miyazaki J., Mishina M., Sakimura K. Purkinje cell-specific and inducible gene recombination system generated from C57BL/6 mouse ES cells. Biochem. Biophys. Res. Commun. 2001, 281:1134-1140.
51. Koshinsky H.A., Lee E., Ow D.W. Cre-lox site-specific recombination between Arabidopsis and tobacco chromosomes. Plant J. 2000, 23:715-722.
52. Kucera G.T., Bortner D.M., Rosenberg M.P. Overexpression of an agouti cDNA in the skin of transgenic mice recapitulates dominant coat color phenotypes of spontaneous mutants. Dev. Biol. 1996, 173:162-173.
53. Lakso M., Sauer B., Mosinger B., Lee E.J., Manning R.W., Yu S.H., Mulder K.L., Westphal H. Targeted oncogene activation by site-specific recombination in transgenic mice. Proc. Natl. Acad. Sci. USA 1992, 89:6232-6236.
54. Lakso M., Pichel J.G., Gorman J.R., Sauer B., Okamoto Y., Lee E., Alt F.W., Westphal H. Efficient in vivo manipulation of mouse genomic sequences at the zygote stage. Proc. Natl. Acad. Sci. USA 1996, 93:5860-5865.
55. Le Y., Gagneten S., Tombaccini D., Bethke B., Sauer B. Nuclear targeting determinants of the phage P1 cre DNA recombinase. Nucleic Acids Res. 1999, 27:4703-4709.
56. Lee Y.H., Sauer B., Johnson P.F., Gonzalez F.J. Disruption of the c/ebp alpha gene in adult mouse liver. Mol. Cell. Biol. 1997, 17:6014-6022.
57. Lee Y.H., Sauer B., Gonzalez F.J. Laron dwarfism and non-insulin-dependent diabetes mellitus in the Hnf-1alpha knockout mouse. Mol. Cell. Biol. 1998, 18:3059-3068.
58. Levin S.D., Anderson S.J., Forbush K.A., Perlmutter R.M. A dominant-negative transgene defines a role for p56lck in thymopoiesis. EMBO J. 1993, 12:1671-1680.
59. Li X., Wang W., Lufkin T. Dicistronic LacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes. Biotechniques 1997, 23:874-878. 880.
60. Li Z.W., Stark G., Gotz J., Rulicke T., Gschwind M., Huber G., Muller U., Weissmann C. Generation of mice with a 200-kb amyloid precursor protein gene deletion by Cre recombinase-mediated site-specific recombination in embryonic stem cells. Proc. Natl. Acad. Sci. USA 1996, 93:6158-6162.
61. Liu J.L., Grinberg A., Westphal H., Sauer B., Accili D., Karas M., LeRoith D. Insulinlike growth factor-I affects perinatal lethality and postnatal development in a gene dosage-dependent manner: manipulation using the Cre/loxP system in transgenic mice. Mol. Endocrinol. 1998, 12:1452-1462.
62. Liu J.L., Yakar S., LeRoith D. Conditional knockout of mouse insulin-like growth factor-1 gene using the Cre/loxP system. Proc. Soc. Exp. Biol. Med. 2000, 223:344-351.
63. Lobe C.G., Koop K.E., Kreppner W., Lomeli H., Gertsenstein M., Nagy A. Z/AP, a double reporter for cre-mediated recombination. Dev. Biol. 1999, 208:281-292.
64. Mansour S.L., Thomas K.R., Capecchi M.R. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 1988, 336:348-352.
65. Martin G.R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. USA 1981, 78:7634-7638.
66. Matsuzaki H., Nakajima R., Nishiyama J., Araki H., Oshima Y. Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid. J. Bacteriol. 1990, 172:610-618.
67. McKnight R.A., Shamay A., Sankaran L., Wall R.J., Hennighausen L. Matrix attachment regions impart position independent regulation of a tissue specific gene in transgenic mice. Proc. Natl. Acad. Sci. USA 1992, 89:6943-6947.
68. Medberry S.L., Dale E., Qin M., Ow D.W. Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination. Nucleic Acids Res. 1995, 23:485-490.
69. Mertens G., Fuss H., Kahmann R. Purification and properties of the DNA invertase Gin encoded by bacteriophage Mu. J. Biol. Chem. 1986, 261:15668-15672.
70. Mertens G., Klippel A., Fuss H., Blocker H., Frank R., Kahmann R. Site-specific recombination in bacteriophage Mu: characterization of binding sites for the DNA invertase Gin. EMBO J. 1988, 7:1219-1227.
71. Metsaranta M., Garofalo S., Decker G., Rintala M., de Crombrugghe B., Vuorio E. Chondrodysplasia in transgenic mice harboring a 15-amino acid deletion in the triple helical domain of pro alpha 1(II) collagen chain. J. Cell. Biol. 1992, 118:203-212.
72. Metzger D., Clifford J., Chiba H., Chambon P. Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Ore recombinase. Proc. Natl. Acad. Sci. USA 1995, 92:6991-6995.
73. Meyers E.N., Lewandoski M., Martin G.R. An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat. Genet. 1998, 18:136-141.
74. Mills A.A., Bradley A. From mouse to man: generating megabase chromosome rearrangements. Trends Genet. 2001, 17:331-339.
75. Novak A., Guo C., Yang W., Nagy A., Lobe C.G. Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis 2000, 28:147-155.
76. Palmiter R.D., Brinster R.L., Hammer R.E., Trumbauer M.E., Rosenfeld M.G., Birnberg N.C., Evans R.M. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 1982, 300:611-615.
77. Peters K., Werner S., Liao X., Wert S., Whitsett J., Williams L. Targeted expression of a dominant negative FGF receptor blocks branching morphogenesis and epithelial differentiation of the mouse lung. EMBO J. 1994, 13:3296-3301.
78. Qin M., Bayley C., Stockton T., Ow D.W. Cre recombinase-mediated site-specific recombination between plant chromosomes. Proc. Natl. Acad. Sci. USA 1994, 91:1706-1710.
79. Qin M., Lee E., Zankel T., Ow D.W. Site-specific cleavage of chromosomes in vitro through Cre-lox recombination. Nucleic Acids Res. 1995, 23:1923-1927.
80. Ray P., Higgins K.M., Tan J.C., Chu T.Y., Yee N.S., Nguyen H., Lacy E., Besmer P. Ectopic expression of a c-kitW42 minigene in transgenic mice: recapitulation of W phenotypes and evidence for c-kit function in melanoblast progenitors. Genes Dev. 1991, 5:2265-2273.
81. Reichardt H.M., Tronche F., Bauer A., Schutz G. Molecular genetic analysis of glucocorticoid signaling using the Cre/loxP system. Biol. Chem. 2000, 381:961-964.
82. Rucker E.B., Piedrahita J.A. Cre-mediated recombination at the murine whey acidic protein (mWAP) locus. Mol. Reprod. Dev. 1997, 48:324-331.
83. Sauer B. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 1987, 7:2087-2096.
84. Sauer B., Henderson N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 1990, 2:441-449.
85. Schwenk F., Baron U., Rajewsky K. A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells. Nucleic Acids Res. 1995, 23:5080-5081.
86. Schwenk F., Sauer B., Kukoc N., Hoess R., Muller W., Kocks C., Kuhn R., Rajewsky K. Generation of Cre recombinase-specific monoclonal antibodies, able to characterize the pattern of Cre expression in Cre-transgenic mouse strains. J. Immunol. Methods 1997, 207:203-212.
87. Seidl K.J., Bottaro A., Vo A., Zhang J., Davidson L., Alt F.W. An expressed neo(r) cassette provides required functions of the 1gamma2b exon for class switching. Int. Immunol. 1998, 10:1683-1692.
88. Shibata H., Kanamaru R., Noda T. Modification of gene targeting method for functional analysis of the target gene in vivo [trans.]. Gan To Kagaku Ryoho 1997, 24:460-465.
89. Smith A.J., De Sousa M.A., Kwabi-Addo B., Heppell-Parton A., Impey H., Rabbitts P. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nature Genet. 1995, 9:376-385.
90. Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat. Genet. 1999, 21:70-71.
91. Soukharev S., Miller J.L., Sauer B. Segmental genomic replacement in embryonic stem cells by double lox targeting. Nucleic Acids Res. 1999, 27:E21.
92. Sternberg N., Hamilton D. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. J. Mol. Biol. 1981, 150:467-486.
93. Sternberg N., Sauer B., Hoess R., Abremski K. Bacteriophage P1 cre gene and its regulatory region. Evidence for multiple promoters and for regulation by DNA methylation. J. Mol. Biol. 1986, 187:197-212.
94. Su H., Wang X., Bradley A. Nested chromosomal deletions induced with retroviral vectors in mice. Nature Genet. 2000, 24:92-95.
95. Sunaga S., Maki K., Komagata Y., Ikuta K., Miyazaki J.I. Efficient removal of loxP-flanked DNA sequences in a gene-targeted locus by transient expression of Cre recombinase in fertilized eggs. Mol. Reprod. Dev. 1997, 46:109-113.
96. Taniguchi M., Sanbo M., Watanabe S., Naruse I., Mishina M., Yagi T. Efficient production of Cre-mediated site-directed recombinants through the utilization of the puromycin resistance gene, pac: a transient gene-integration marker for ES cells. Nucleic Acids Res. 1998, 26:679-680.
97. te Riele H., Maandag E.R., Berns A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl. Acad. Sci. USA 1992, 89:5128-5132.
98. Thomas K.R., Capecchi M.R. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 1987, 51:503-512.
99. Thompson S., Clarke A.R., Pow A.M., Hooper M.L., Melton D.W. Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Cell 1989, 56:313-321.
100. Vallier L., Mancip J., Markossian S., Lukaszewicz A., Dehay C., Metzger D., Chambon P., Samarut J., Savatier P. An efficient system for conditional gene expression in embryonic stem cells and in their in vitro and in vivo differentiated derivatives. Proc. Natl. Acad. Sci. USA 2001, 98:2467-2472.
101. Van Deursen J., Fornerod M., Van Rees B., Grosveld G. Cre-mediated site-specific translocation between nonhomologous mouse chromosomes. Proc. Natl. Acad. Sci. USA 1995, 92:7376-7380.
102. Vazquez J.C., Nogues C., Rucker E.B., Piedrahita J.A. Factors affecting the efficiency of introducing precise genetic changes in ES cells by homologous recombination: tag-and-exchange versus the Cre-loxP system. Transgenic Res. 1998, 7:181-193.
103. Verrou C., Zhang Y., Zurn C., Schamel W.W., Reth M. Comparison of the tamoxifen regulated chimeric Cre recombinases MerCreMer and CreMer. Biol. Chem. 1999, 380:1435-1438.
104. Werner S., Weinberg W., Liao X., Peters K.G., Blessing M., Yuspa S.H., Weiner R.L., Williams L.T. Targeted expression of a dominant-negative FGF receptor mutant in the epidermis of transgenic mice reveals a role of FGF in keratinocyte organization and differentiation. EMBO J. 1993, 12:2635-2643.
105. Wierzbicki A., Kendall M., Abremski K., Hoess R. A mutational analysis of the bacteriophage P1 recombinase Cre. J. Mol. Biol. 1987, 195:785-794.
106. Xu X., Li C., Garrett-Beal L., Larson D., Wynshaw-Boris A., Deng C.X. Direct removal in the mouse of a floxed neo gene from a three-loxP conditional knockout allele by two novel approaches. Genesis 2001, 30:1-6.
107. Yakar S., Liu J.L., Stannard B., Butler A., Accili D., Sauer B., LeRoith D. Normal growth and development in the absence of hepatic insulin-like growth factor I. Proc. Natl. Acad. Sci. USA 1999, 96:7324-7329.
108. Yu H., Kessler J., Shen J. Heterogeneous populations of ES cells in the generation of a floxed Presenilin-1 allele. Genesis 2000, 26:5-8.
109. Zakany J., Duboule D. Synpolydactyly in mice with a targeted deficiency in the HoxD complex. Nature 1996, 384:69-71.
110. Zheng B., Mills A.A., Bradley A. A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice. Nucleic Acids Res. 1999, 27:2354-2360.
111. Zheng B., Sage M., Cai W.W., Thompson D.M., Tavsanli B.C., Cheah Y.C., Bradley A. Engineering a mouse balancer chromosome. Nature Genet. 1999, 22:375-378.
112. Zheng B., Sage M., Sheppeard E.A., Jurecic V., Bradley A. Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications. Mol. Cell. Biol. 2000, 20:648-655.
113. Zheng B., Mills A.A., Bradley A. Introducing defined chromosomal rearrangements into the mouse genome. Methods 2001, 24:81-94.
114. Zimmer A., Gruss P. Production of chimaeric mice containing embryonic stem (ES) cells carrying a homeobox Hox1.1 allele mutated by homologous recombination. Nature 1989, 338:150-153.