Dicistronic LacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes

BioTechniques

Volumn 23, Issue 5, 1997, Pages 874-882

  Li, Xue ^a   Wang, Weidong ^a   Lufkin, Thomas ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CISTRON; ENCEPHALOMYOCARDITIS VIRUS; GENE FUSION; GENETIC ANALYSIS; GENETIC ENGINEERING; NONHUMAN; REPORTER GENE; TRANSGENE; TRANSLATION REGULATION;

ENCEPHALOMYOCARDITIS VIRUS; MAMMALIA; MUS MUSCULUS;

EID: 0030698846     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97235st03     Document Type: Article

References (19)

1. Bober, E., C. Baum, T. Braun and H.H. Arnold. 1994. A novel NK-related mouse homeobox gene: expression in central and peripheral nervous structures during embryonic development. Dev. Biol. 162:288-303.
2. Fields-Berry, S.C., A.L. Halliday and C.L. Cepko. 1992. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA 89:693-691.
3. Frasch, M., X. Chen and T. Lufkin. 1995. Evolutionary-conserved enhancers direct region-specific expression of the murine Hoxa-1 and Hoxa-2 loci in both mice and Drosophila. Development 121:957-974.
4. Green, S., I. Issemann and E. Sheer. 1988. A versatile in vivo and in vitro eukaryotic expression vector for protein engineering. Nucleic Acids Res. 16:369.
5. Grosveld, F. and G. Kollias (Eds.). 1992. Transgenic Animals. Academic Press, San Diego.
6. Hall, C.V., P.E. Jacob, G.M. Ringold and F. Lee. 1983. Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells. J. Mol. Appl. Genet. 2:101-109.
7. Hogan, B., R. Beddington, F. Costantini and E. Lacy. 1994. Manipulating the Mouse Embryo: A Laboratory Press Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
8. Hughes, S.H., J.J. Greenhouse, C.J. Petropoulos and P. Sutrave. 1987. Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors. J. Virol. 61:3004-3012.
9. Itasaki, N., J. Sharpe, A. Morrison and R. Krumlauf. 1996. Reprogramming Hox expression in the vertebrate hindbrain: influence of paraxial mesoderm and rhombomere transposition. Neuron 16:487-500.
10. Kim, D.G., H.M. Kang, S.K. Jang and H.S. Shin. 1992. Construction of a bifunctional mRNA in the mouse by using the internal ribosomal entry site of the encephalomyocarditis virus. Mol. Cell. Biol. 12:3636-3643.
11. Kozak, M. 1994. Determinants of translational fidelity and efficiency in vertebrate mRNAs. Biochimie 76:815-821.
12. Mountford, P., B. Zevnik, A. Düwel, J. Nichols, M. Li, C. Dani, M. Robertson, I. Chambers and A. Smith. 1994. Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression. Proc. Natl. Acad. Sci. USA 91:4303-4307.
13. Mountford, P.S. and A.G. Smith. 1995. Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis. Trends Genet. 11:179-184.
14. Pinkert, C.A. (Ed.). 1994. Transgenic Animal Technology. Academic Press, San Diego.
15. Rinkwitz-Brandt, S., M. Justus, I. Oldenettel, H.H. Arnold and E. Bober. 1995. Distinct temporal expression of mouse Nkx-5.1 and Nkx-5.2 homeobox genes during brain and ear development. Mech. Dev. 52:371-381.
16. Sauer, B. 1987. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7:2087-2096.
17. Sauer, B. 1993. Manipulation of transgenes by site-specific recombination: use of Cre recombinase, p. 890-900. In P.M. Wassarman, and M.L. Depamphilis (Eds.), Guide to Techniques in Mouse Development. Vol. 225. Academic Press, San Diego.
18. Sauer, B. and N. Henderson. 1989. Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome. Nucleic Acids Res. 17:147-161.
19. Stadler, H.S., J.C. Murray, N.J. Leysens, P.J. Goodfellow and M. Solursh. 1995. Phylogenetic conservation and physical mapping of members of the H6 homeobox gene family. Mamm. Genome 6:383-388.