Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 33, 2015, Pages 10354-10358

  Marous, Daniel R ^a   Lloyd, Evan P ^b   Buller, Andrew R ^a   Moshos, Kristos A ^b   Grove, Tyler L ^c   Blaszczyk, Anthony J ^c   Booker, Squire J ^c   Townsend, Craig A ^b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Carbapenem; Cobalamin; Methylase; Radical SAM; lactam antibiotics

Indexed keywords

5' AMINO 5' DEOXYADENOSINE; CARBAPENEM; COBALAMIN; COENZYME A; CYANOCOBALAMIN; PANTETHEINE; RADICAL S ADENOSYLMETHIONINE; S ADENOSYLMETHIONINE; SULFUR; THIENAMYCIN; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; BETA LACTAM; CARBAPENEM DERIVATIVE; CEPHALOSPORIN DERIVATIVE; PENICILLIN DERIVATIVE; THIENAMYCIN DERIVATIVE;

AMINO TERMINAL SEQUENCE; ARTICLE; CHEMICAL BOND; DIASTEREOISOMER; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; EPIMERIZATION; FATTY ACID DESATURATION; IN VITRO STUDY; METHYLATION; NONHUMAN; PRIORITY JOURNAL; STEREOCHEMISTRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; BIOSYNTHESIS; CATALYSIS; CHEMISTRY; DNA METHYLATION; DRUG DESIGN; ESCHERICHIA COLI; FERMENTATION; LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; STREPTOMYCES; TANDEM MASS SPECTROMETRY;

ANTI-BACTERIAL AGENTS; BETA-LACTAMS; CARBAPENEMS; CATALYSIS; CEPHALOSPORINS; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; DNA METHYLATION; DRUG DESIGN; ESCHERICHIA COLI; FERMENTATION; METHYLATION; PENICILLINS; S-ADENOSYLMETHIONINE; STREPTOMYCES; TANDEM MASS SPECTROMETRY; THIENAMYCINS; VITAMIN B 12;

EID: 84940184520     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1508615112     Document Type: Article

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