Isolated Conglutin γ from Lupin, but not Phytate, Lowers Serum Cholesterol Without Influencing Vascular Lesion Development in the ApoE-deficient Mouse Model

Plant Foods for Human Nutrition

Volumn 70, Issue 2, 2015, Pages 113-118

  Radtke, Juliane ^a   Schutkowski, Alexandra ^a   Brandsch, Corinna ^a   Hirche, Frank ^a   Hasenkopf, Katrin ^b   Stangl, Gabriele I ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b FRAUNHOFER INSTITUTE FOR PROCESS ENGINEERING AND PACKAGING IVV   (Germany)

Author keywords

Cholesterol; Conglutin ; Lupin; Mouse; Phytate; Vascular lesions

Indexed keywords

7 DEHYDROCHOLESTEROL; 7-DEHYDROCHOLESTEROL; APOLIPOPROTEIN E; BIOLOGICAL MARKER; CHOLESTEROL; CONGLUTIN-GAMMA PROTEIN, LUPINUS ANGUSTIFOLIUS; PHYTIC ACID; PLANT PROTEIN; TRACE ELEMENT; VITAMIN D;

ANIMAL; BLOOD; CHEMISTRY; DISEASE MODEL; ISOLATION AND PURIFICATION; KNOCKOUT MOUSE; LUPIN; MALE; MOUSE;

ANIMALS; APOLIPOPROTEINS E; BIOMARKERS; CHOLESTEROL; DEHYDROCHOLESTEROLS; DISEASE MODELS, ANIMAL; LUPINUS; MALE; MICE; MICE, KNOCKOUT; PHYTIC ACID; PLANT PROTEINS; TRACE ELEMENTS; VITAMIN D;

EID: 84939980819     PISSN: 09219668     EISSN: 15739104     Source Type: Journal    
DOI: 10.1007/s11130-015-0481-x     Document Type: Article

References (34)

1. Cerletti P, Duranti M, Restan P (1983) Properties of lupine proteins relevant to their nutritional performance. Plant Foods Hum Nutr 32:145–154
2. Bettzieche A, Brandsch C, Weisse K, Hirche F, Eder K, Stangl GI (2008) Lupin protein influences the expression of hepatic genes involved in the fatty acid synthesis and triacylglycerol hydrolysis of adult rats. Br J Nutr 99:952–962
3. Brandsch C, Kappis D, Weisse K, Stangl GI (2010) Effects of untreated and thermally treated lupin protein on plasma and liver lipids of rats fed a hypercholesterolemic high fat or high carbohydrate diet. Plant Foods Hum Nutr 65:410–446. doi:10.1007/s11130-010-0201-5
4. Fontanari GG, Batistuti JP, da Cruz RJ, Saldiva PHN, Arêas JAG (2012) Cholesterol-lowering effect of whole lupin (Lupinus albus) seed and its protein isolate. Food Chem 132:1521–1526
5. Weisse K, Brandsch C, Zernsdorf B, Nkengfack Nembongwe GS, Hofmann K, Eder K, Stangl GI (2010) Lupin protein compared to casein lowers the LDL cholesterol:HDL cholesterol-ratio of hypercholesterolemic adults. Eur J Nutr 49:65–71
6. Bähr M, Fechner A, Krämer J, Kiehntopf M, Jahreis G (2013) Lupin protein positively affects plasma LDL cholesterol and LDL:HDL cholesterol ratio in hypercholesterolemic adults after four weeks of supplementation: a randomized, controlled crossover study. Nutr J 12:107. doi:10.1186/1475-2891-12-107
7. Marchesi M, Parolini C, Diani E, Rigamonti E, Cornelli L, Arnoldi A, Sirtori CR, Chiesa G (2008) Hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model. Br J Nutr 100:707–710
8. Weisse K, Brandsch C, Hirche F, Eder K, Stangl GI (2010) Lupin protein isolate and cysteine-supplemented casein reduce calcification of atherosclerotic lesions in apoE-deficient mice. Br J Nutr 103:180–188
9. Sirtori CR, Lovati MR, Manzoni C, Castiglioni S, Duranti M, Magni C, Morandi S, D’Agostina A, Arnoldi A (2004) Proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase LDL receptor activity in HepG2 cells. J Nutr 134:18–23
10. Schlemmer U, Frølich W, Prieto RM, Grases F (2009) Phytate in foods and significance for humans: food sources, intake, processing, bioavailability, protective role and analysis. Mol Nutr Food Res 53:S330–S375
11. Rimbach G, Pallauf J, Brandt K, Most E (1995) Effect of phytic acid and microbial phytase on Cd accumulation, Zn status, and apparent absorption of Ca, P, Mg, Fe, Zn, Cu, and Mn in growing rats. Ann Nutr Metab 39:361–370
12. Pallauf J, Rimbach G (1997) Nutritional significance of phytic acid and phytase. Arch Tierernahr 50:301–319
13. Onomi S, Okazaki Y, Katayama T (2004) Effect of dietary level of phytic acid on hepatic and serum lipid status in rats fed a high-sucrose diet. Biosci Biotechnol Biochem 68:1379–1381
14. Lee SH, Park HJ, Cho SY, Jung HJ, Cho SM, Cho YS, Lillehoj HS (2005) Effects of dietary phytic acid on serum and hepatic lipid levels in diabetic KK mice. Nutr Res 25:869–876
15. Radtke J, Geissler S, Schutkowski A, Brandsch C, Kluge H, Duranti MM, Keller S, Jahreis G, Hirche F, Stangl GI (2014) Lupin protein isolate versus casein modifies cholesterol excretion and mRNA expression of intestinal sterol transporters in a pig model. Nutr Metab 11:9. doi:10.1186/1743-7075-11-9
16. Grases F, Prieto RM, Sanchis P, Saus C, De Francisco T (2008) Role of phytate and osteopontin in the mechanism of soft tissue calcification. J Nephrol 21:768–775
17. Grases F, Sanchis P, Perello J, Isern B, Prieto RM, Fernández-Palomeque C, Torres JJ (2007) Effect of crystallization inhibitors on vascular calcifications induced by vitamin D – a pilot study in Sprague–Dawley rats. Circ J 71:1152–1156
18. Zaragoza C, Gomez-Guerrero C, Martin-Ventura JL, Blanco-Colio L, Lavin B, Mallavia B, Tarin C, Mas S, Ortiz A, Egido J (2011) Animal models of cardiovascular diseases. J Biomed Biotechnol 2011:497841. doi:10.1155/2011/497841
19. Pendse AA, Arbones-Mainar JM, Johnson LA, Altenburg MK, Maeda N (2009) Apolipoprotein E knock-out and knock-in mice: atherosclerosis, metabolic syndrome, and beyond. J Lipid Res 50(Suppl):S178–S182. doi:10.1194/jlr. R800070-JLR200
20. D-Agostina A, Antonioni C, Resta D, Arnoldi A, Bez J, Knauf U, Wäsche A (2006) Optimization of a pilot-scale process for producing lupin protein isolates with valuable technological properties and minimum thermal damage. J Agric Food Chem 54:92–98
21. Bassler R (1976) Die chemische Untersuchung von Futtermitteln, 3rd edn. vol. 3. Methodenbuch. VDLUFA-Verlag, Darmstadt
22. Eder K, Peganova S, Kluge H (2001) Studies on the tryptophan requirement of piglets. Arch Tierernahr 55:281–297
23. Fontaine J, Bech-Andersen S, Bütikofer U, Froidmont-Göritz I (1998) Determination of tryptophan in feed by HPLC – development of an optimal hydrolysis and extraction procedure by the EU commission DG XII in three international collaborative studies. Agribiol Res 51:97–108
24. Harland BF, Oberleas D (1986) Anion exchange method for determination of phytate in foods: collaborative study. J Assoc Off Anal Chem 69:667–670
25. National Research Council (NRC), Committee on Animal Nutrition (1995) Nutrient requirements of laboratory animals. National Academy Press, Washington
26. Honda A, Yamashita K, Hara T, Ikegami T, Miyazaki T, Shirai M, Xu G, Numazawa M, Matsuzaki Y (2009) Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MS. J Lipid Res 50:350–357
27. Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE (1995) Contents of cholecalciferol, ergocalciferol, and their 25-hydroxylated metabolites in milk products and raw meat and liver as determined by HPLC. J Agric Food Chem 43:2394–2399
28. Huynh NN, Chin-Dusting J (2006) Amino acids, arginase and nitric oxide in vascular health. Clin Exp Pharmacol Physiol 33:1–8
29. Hirche F, Schröder A, Knoth B, Stangl GI, Eder K (2006) Effect of dietary methionine on plasma and liver cholesterol concentrations in rats and expression of hepatic genes involved in cholesterol metabolism. Br J Nutr 95:879–888
30. Foley RC, Gao LL, Spriggs A, Soo LY, Goggin DE, Smith PM, Atkins CA, Singh KB (2011) Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius. BMC Plant Biol 11:59. doi:10.1186/1471-2229-11-59
31. Lovati MR, Manzoni C, Castiglioni S, Parolari A, Magni C, Duranti M (2012) Lupin seed γ-conglutin lowers blood glucose in hyperglycaemic rats and increases glucose consumption of HepG2 cells. Br J Nutr 107:67–73. doi:10.1017/S0007114511002601
32. Mardiewicz LH, Honke J, Haros M, Swiatecka D, Wróblewska B (2013) Diet shapes the ability of human intestinal microbiota to degrade phytate – in vitro studies. J Appl Microbiol 115:247–259. doi:10.1111/jam.12204
33. Miettinen TA, Gylling H, Nissinen MJ (2011) The role of serum non-cholesterol sterols as surrogate markers of absolute cholesterol synthesis and absorption. Nutr Metab Cardiovasc Dis 21:765–769. doi:10.1016/j.numecd.2011.05.005
34. Xu L, Korade Z, Porter NA (2010) Oxysterols from free radical chain oxidation of 7-dehydrocholesterol: product and mechanistic studies. J Am Chem Soc 132:2222–2232. doi:10.1021/ja9080265