Lupin seed γ-conglutin lowers blood glucose in hyperglycaemic rats and increases glucose consumption of HepG2 cells

British Journal of Nutrition

Volumn 107, Issue 1, 2012, Pages 67-73

  Rosa Lovati, Maria ^a   Manzoni, Cristina ^a   Castiglioni, Silvia ^a   Parolari, Anna ^a   Magni, Chiara ^a   Duranti, Marcello ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Conglutin; Glucose consumption; HepG2 cells; Hyperglycaemic rats; Lupinus albus seed protein

Indexed keywords

CONGLUTININ; DRINKING WATER; GAMMA CONGLUTININ; GLUCOSE; INSULIN; METFORMIN; RECOMBINANT HUMAN INSULIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL STRAIN HEPG2; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; DIET RESTRICTION; GLUCOSE BLOOD LEVEL; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; HOMEOSTASIS; HUMAN; HUMAN CELL; HYPERGLYCEMIA; IN VITRO STUDY; INCUBATION TIME; INSULIN BLOOD LEVEL; INSULIN RESISTANCE; INSULIN RESPONSE; LUPIN; MALE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; ORAL GLUCOSE TOLERANCE TEST; PLANT SEED; RAT; TREATMENT DURATION;

ANIMALS; BLOOD GLUCOSE; DIABETES MELLITUS, TYPE 2; DIETARY PROTEINS; DIETARY SUPPLEMENTS; GLUCOSE; HEP G2 CELLS; HEPATOCYTES; HUMANS; HYPERGLYCEMIA; HYPOGLYCEMIC AGENTS; INSULIN; INSULIN RESISTANCE; LUPINUS; MALE; METFORMIN; PLANT PROTEINS; RATS; RATS, SPRAGUE-DAWLEY; SEEDS; TIME FACTORS;

ANIMALIA; LUPINUS ALBUS; RATTUS;

EID: 84855821397     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S0007114511002601     Document Type: Article

References (36)

1. Lovati MR, Manzoni C, Gianazza E, et al. (2000) Soy protein peptides regulate cholesterol homeostasis in HepG2 cells. J Nutr 130, 2543-2549.
2. Duranti M, Lovati MR, Dani V, et al. (2004) The a0 subunit from soybean 7S globulin lowers plasma lipids and up-regulates VLDL-liver receptors in rats fed a hypercholes-terolemic diet. J Nutr 134, 1334-1339. (Pubitemid 38720703)
3. Castiglioni S, Manzoni C, D'Uva A, et al. (2003) Soy proteins reduce progression of a focal lesion and lipoprotein oxidability in rabbits fed on a cholesterol-rich diet. Atherosclerosis 171, 163-170. (Pubitemid 37486068)
4. Sirtori CR, Lovati MR, Manzoni C, et al. (2004) Proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase LDL receptor activity in HepG2 cells. J Nutr 134, 18-23. (Pubitemid 38057417)
5. Manzoni C, Duranti M, Eberini I, et al. (2003) Subcellular localization of soybean 7S globulin in HepG2 cells and LDL receptor up-regulation by its a0 constituent subunit. J Nutr 133, 2149-2155. (Pubitemid 36841531)
6. Consonni A, Lovati MR, Manzoni C, et al. (2009) Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin a0 subunit involved in HepG2 cell cholesterol homeostasis. J Nutr Biochem; 21, 887-891.
7. Cho SJ, Juillerat MA & Lee CH (2008) Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes. J Agric Food Chem 56, 4372-4376. (Pubitemid 351999358)
8. Scarafoni A, Magni C & Duranti M (2007) Molecular nutra-ceutics as a mean to investigate the positive effects of legume seed proteins on human health. Trends Food Sci Technol 18, 454-463. (Pubitemid 47176360)
9. Restani P, Duranti M, Cerletti P, et al. (1981) Subunit composition of the seed globulins of Lupinus albus. Phytochem 20, 2077-2083.
10. Blagrove RJ, Gillespie JM, Lilley GG, et al. (1980) Physico-chemical studies on conglutin g, a storage globulin from seeds of Lupinus angustifolius. Aust J Plant Physiol 7, 1-13.
11. Kagawa H, Yamauchi F & Hirano H (1987) Soybean basic 7S globulin represents a protein widely distributed in legume species. FEBS Lett 226, 145-149.
12. Satoh S, Sturm A, Fuji T, et al. (1992) cDNA cloning of an extracellular dermal glycoprotein of carrot and its expression in response to wounding. Planta 188, 432-438.
13. Magni C, Sessa F, Accardo E, et al. (2004) Conglutin g, a lupin seed protein, binds insulin in vitro and reduces plasma glucose levels of hyperglycemic rats. J Nutr Biochem 15, 646-650. (Pubitemid 39611362)
14. Hanada K & Hirano H (2004) Interaction of a 43-kDa receptor-like protein with a 4-kDa hormone-like peptide in soybean. Biochemistry 43, 12105-12112. (Pubitemid 39280554)
15. Midaoui AE, Elimadi A, Wu L, et al. (2003) Lipoic acid prevents hypertension, hyperglycemia, and the increase in heart mitochondrial superoxide production. Am J Hypertens 16, 173-179. (Pubitemid 36293060)
16. Bez J, Schott M, Knauf U, et al. (2005) Protein isolates from sweet white lupin seeds: pilot production and techno-func-tional properties. In Optimized Processing for Preparing Healthy and Added Value Food Ingredients from Lupin Kernel, the European Protein-rich Grain Legume, pp. 21-35 [A Arnoldi, editor]. Rome: Aracne Editrice SRL.
17. Duranti M, Scarafoni A, Gius C, et al. (1994) Heat induced synthesis and tunicamycin-sensitive secretion of the putative storage glycoprotein conglutin g from mature lupin seeds. Eur J Biochem 222, 387-393. (Pubitemid 24204907)
18. Capraro J, Spotti P, Magni C, et al. (2010) Spectroscopic studies on the pH-dependent structural dynamics of g-conglutin, the blood glucose-lowering protein of lupin seeds. Int J Biol Macromol 47, 502-507.
19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 660-665.
20. Midaoui AE & De Champlain J (2002) Prevention of hypertension, insulin resistance, and oxidative stress by a-lipoic acid. Hypertension 39, 303-307. (Pubitemid 34165233)
21. Li YY, Wu HS, Tang L, et al. (2007) The potential insulin sensitizing and glucose lowering effects of a novel indole derivative in vitro and in vivo. Pharmacol Res 56, 335-343. (Pubitemid 47496225)
22. Duranti M, Consonni A, Magni C, et al. (2008) The major proteins of lupin seed: characterisation and molecular properties for use as functional and nutraceutical ingredients. Trends Food Sci Technol 19, 624-633.
23. King H, Aubert RE & Herman WH (1998) Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care 21, 1414-1431. (Pubitemid 28405191)
24. Parikh N, Pencina M, Wang T, et al. (2007) Increasing trends in incidence of overweight and obesity over 5 decades. Am J Med 120, 242-250.
25. Duranti M (2006) Grain legume proteins and nutraceutical properties. Fitoterapia 77, 67-82. (Pubitemid 43220250)
26. Champ M (2001) Benefits of pulses in human diet. In Proceedings of 4th European Conference on Grain Legumes, pp. 109-113. Valladolid: AEP.
27. Ferranini A & Pirolli M (1937) L'azione del decotto di semi di Lupinus albus sulla curva glicemica da carico di glucosio nei soggetti normali e diabetici. Folia Med 23, 729-748.
28. Orestano G (1940) Sull'azione ipoglicemica dei semi di Lupinus albus. Arch Farmacol Sperim 70, 113-117.
29. Kordowiak AM, Dudek B & Grybo R (2004) Influence of sodium bis (oxalato)oxovanadate (IV) on phospholipids in liver Golgi fraction from control and streptozotocin-diabetic rats. Comp Biochem Physiol C Toxicol Pharmacol 125, 11-16.
30. Pari L & Saravanan G (2002) Antidiabetic effect of Cogent db, a herbal drug in alloxan-induced diabetes mellitus. Comp Biochem Physiol C Toxicol Pharmacol 131, 19-25. (Pubitemid 34131573)
31. Terruzzi I, Senesi P, Magni C, et al. (2009) Insulin-mimetic action of conglutin-g, a lupin seed protein, in mouse myo-blasts. Nutr Metab Cardiovasc Dis 21, 197-205.
32. Capraro J, Magni C, Scarafoni A, et al. (2009) Susceptibility of lupin g-conglutin, the plasma glucose-lowering protein of lupin seeds, to proteolytic enzymes. J Agric Food Chem 57, 8612-8616.
33. Zhang W, Xu YC, Guo FJ, et al. (2008) Anti-diabetic effects of cinnamaldehyde and berberine and their impacts on retinol-binding protein 4 expression in rats with type 2 diabetes mellitus. Chin Med J 121, 2124-2128.
34. Lovati MR, Manzoni C, Castiglioni S, et al. (2006) Soy peptide and cholesterol homeostasis. FASEB J 18, A86.
35. Lavigne C, Marette A & Jacques H (2000) Cod and soy proteins compared with casein improve glucose tolerance and insulin sensitivity in rats. Am J Physio Endocrinol Metab 278, E491-E500. (Pubitemid 30149394)
36. Nicolucci A & Rossi MC (2008) Incretin-based therapies: a new potential treatment approach to overcome clinical inertia in type 2 diabetes. Acta Biomed 79, 184-191.