Multiplex Quantification of 12 European Union Authorized Genetically Modified Maize Lines with Droplet Digital Polymerase Chain Reaction

Analytical Chemistry

Volumn 87, Issue 16, 2015, Pages 8218-8226

  Dobnik, David ^a   Spilsberg, Bjørn ^b   Bogožalec Košir, Alexandra ^a,c   Holst Jensen, Arne ^b   Žel, Jana ^a  

^a NATIONAL INSTITUTE OF BIOLOGY   (Slovenia)

^b NORWEGIAN VETERINARY INSTITUTE   (Norway)

^c J Stefan International Postgraduate School   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

CHAINS; COST BENEFIT ANALYSIS; COST EFFECTIVENESS; DROPS; GENETICALLY MODIFIED PLANTS; MULTIPLEXING;

GENETICALLY MODIFIED MAIZE; GENETICALLY MODIFIED ORGANISMS; LIMIT OF QUANTIFICATIONS; MULTIPLEX ASSAYS; PERFORMANCE PARAMETERS; QUANTIFICATION METHODS; QUANTITATIVE POLYMERASE CHAIN REACTION; SEQUENCE SIMILARITY;

POLYMERASE CHAIN REACTION;

ECONOMICS; EUROPEAN UNION; GENETICS; MAIZE; MULTIPLEX POLYMERASE CHAIN REACTION; PROCEDURES; REPRODUCIBILITY; TRANSGENIC PLANT;

EUROPEAN UNION; MULTIPLEX POLYMERASE CHAIN REACTION; PLANTS, GENETICALLY MODIFIED; REPRODUCIBILITY OF RESULTS; ZEA MAYS;

EID: 84939818124     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/acs.analchem.5b01208     Document Type: Article

References (36)

1. Gruère, G. P.; Rao, S. R. AgBioForum 2007, 10, 51-64
2. European Commission Off. J. Eur. Union L 2003, 268, 1-23
3. Holst-Jensen, A.; De Loose, M.; Van den Eede, G. J. Agric. Food Chem. 2006, 54, 2799-2809 10.1021/jf052849a
4. European Commission Off. J. Eur. Union L 2004, 348, 18-26
5. Holst-Jensen, A.; Bertheau, Y.; de Loose, M.; Grohmann, L.; Hamels, S.; Hougs, L.; Morisset, D.; Pecoraro, S.; Pla, M.; Van den Bulcke, M.; Wulff, D. Biotechnol. Adv. 2012, 30, 1318-1335 10.1016/j.biotechadv.2012.01.024
6. Morisset, D.; Novak, P. K.; Zupanič, D.; Gruden, K.; Lavrač, N.; Žel, J. BMC Bioinf. 2014, 15, 258 10.1186/1471-2105-15-258
7. Block, A.; Debode, F.; Grohmann, L.; Hulin, J.; Taverniers, I.; Kluga, L.; Barbau-Piednoir, E.; Broeders, S.; Huber, I.; Van den Bulcke, M.; Heinze, P.; Berben, G.; Busch, U.; Roosens, N.; Janssen, E.; Žel, J.; Gruden, K.; Morisset, D. BMC Bioinf. 2013, 14, 256 10.1186/1471-2105-14-256
8. Waiblinger, H.-U.; Grohmann, L.; Mankertz, J.; Engelbert, D.; Pietsch, K. Anal. Bioanal. Chem. 2010, 396, 2065-2072 10.1007/s00216-009-3173-2
9. Huber, I.; Block, A.; Sebah, D.; Debode, F.; Morisset, D.; Grohmann, L.; Berben, G.; Stebih, D.; Milavec, M.; Zel, J.; Busch, U. J. Agric. Food Chem. 2013, 61, 10293-10301 10.1021/jf402448y
10. Waiblinger, H.-U.; Ernst, B.; Anderson, A.; Pietsch, K. Eur. Food Res. Technol. 2008, 226, 1221-1228 10.1007/s00217-007-0748-z
11. Takabatake, R.; Koiwa, T.; Kasahara, M.; Takashima, K.; Futo, S.; Minegishi, Y.; Akiyama, H.; Teshima, R.; Oguchi, T.; Mano, J.; Furui, S.; Kitta, K. Shokuhin Eiseigaku Zasshi 2011, 52, 265-269 10.3358/shokueishi.52.265
12. Sykes, P. J.; Neoh, S. H.; Brisco, M. J.; Hughes, E.; Condon, J.; Morley, A. A. Biotechniques 1992, 13, 444-449
13. Pinheiro, L. B.; Coleman, V. A.; Hindson, C. M.; Herrmann, J.; Hindson, B. J.; Bhat, S.; Emslie, K. R. Anal. Chem. 2012, 84, 1003-1011 10.1021/ac202578x
14. Vogelstein, B.; Kinzler, K. W. Proc. Natl. Acad. Sci. U. S. A. 1999, 96, 9236-9241 10.1073/pnas.96.16.9236
15. Berdal, K. G.; Bøydler, C.; Tengs, T.; Holst-Jensen, A. Eur. Food Res. Technol. 2008, 227, 1149-1157 10.1007/s00217-008-0830-1
16. Lee, D.; Mura, M. La; Greenland, A.; Mackay, I. Food Chem. 2010, 118, 974-978 10.1016/j.foodchem.2008.05.118
17. European Commission. Off. J. Eur. Union 2000, 109, 29-42.
18. European Commission Off. J. Eur. Union 2011, 166, 9-15
19. EURL-GMFF. Technical guidance document from the European Union Reference Laboratory for Genetically Modified Food and Feed on the implementation of Commission Regulation (EU) No. 619/2011, http://gmo-crl.jrc.ec.europa.eu/doc/Technical%20Guidance%20from%20EURL%20on%20LLP.pdf (accessed January 30, 2015).
20. Baker, M. Nat. Methods 2012, 9, 541-544 10.1038/nmeth.2027
21. Demeke, T.; Gräfenhan, T.; Holigroski, M.; Fernando, U.; Bamforth, J.; Lee, S.-J. Food Control 2014, 46, 470-474 10.1016/j.foodcont.2014.06.018
22. Bhat, S.; Herrmann, J.; Armishaw, P.; Corbisier, P.; Emslie, K. R. Anal. Bioanal. Chem. 2009, 394, 457-467 10.1007/s00216-009-2729-5
23. Corbisier, P.; Bhat, S.; Partis, L.; Xie, V. R. D.; Emslie, K. R. Anal. Bioanal. Chem. 2010, 396, 2143-2150 10.1007/s00216-009-3200-3
24. Morisset, D.; Štebih, D.; Milavec, M.; Gruden, K.; Žel, J. PLoS One 2013, 8, e62583 10.1371/journal.pone.0062583
25. Burns, M. J.; Burrell, a. M.; Foy, C. a. Eur. Food Res. Technol. 2010, 231, 353-362 10.1007/s00217-010-1277-8
26. Holst-Jensen, A.; Berdal, K. G. J. AOAC Int. 2004, 87, 927-936
27. International Organization for Standardization. ISO 21570:2005 Foodstuffs-Methods of analysis for the detection of genetically modified organisms and derived products-Quantitative nucleic acid based methods; ISO 21570; International Organization for Standardization: Geneva, Switzerland, 2005.
28. Brodmann, P. D.; Ilg, E. C.; Berthoud, H.; Herrmann, A. J. AOAC Int. 2002, 85, 646-653
29. Rački, N.; Dreo, T.; Gutierrez-Aguirre, I.; Blejec, A.; Ravnikar, M. Plant Methods 2014, 10, 42 10.1186/s13007-014-0042-6
30. Vallone, P. M.; Butler, J. M. BioTechniques 2004, 37, 226-231 10.2144/3702A0226
31. EURL-GMFF. Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing European Network of GMO Laboratories (ENGL), http://gmo-crl.jrc.ec.europa.eu/doc/Min-Perf-Requirements-Analytical-methods.pdf (accessed January 30, 2015)
32. Peccoud, J.; Jacob, C. Biophys. J. 1996, 71, 101-108 10.1016/S0006-3495(96)79205-6
33. Standardization, I. O. for. ISO 24276:2006 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - General requirements and definitions; ISO 24276; International Organization for Standardization: Geneva, Switzerland, 2006.
34. Holst-Jensen, A.; Crespo, T.; Simplicio, A.; Richl, P.; Welsche, M.; Dobnik, D.; Dreo, T. Deliverable 6.1 MPP Decathlon, http://www.decathlon-project.eu/sites/default/files/Deliverable6.1-MPP-Decathlon.pdf.
35. Codex Committee on Methods of Analysis and Sampling. (2010, 1-22.
36. Milavec, M.; Dobnik, D.; Yang, L.; Zhang, D.; Gruden, K.; Zel, J. Anal. Bioanal. Chem. 2014, 406, 6485-6497 10.1007/s00216-014-8077-0