Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

Food Chemistry

Volumn 118, Issue 4, 2010, Pages 974-978

  Lee, David ^a   Mura, Maurizio La ^a,b   Greenland, Andrew ^a   Mackay, Ian ^a  

^a NATIONAL INSTITUTE OF AGRICULTURAL BOTANY   (United Kingdom)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

DNA quantitation; GMO content determination; Lectin; RoundUp Ready soya; Single molecule amplification and detection; Transgene

Indexed keywords

LECTIN;

ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; GENE AMPLIFICATION; GENETICALLY MODIFIED FOOD; GENOTYPE; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SOYBEAN;

EID: 70349414928     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodchem.2008.05.118     Document Type: Article

References (13)

1. Cankar K., Stebih D., Dreo T., Zel J., and Gruden K. Critical points of DNA quantification by real-time PCR - effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnology 6 (2006) 37
2. Dear P.H., and Cook P.R. Happy mapping: A proposal for linkage mapping the human genome. Nucleic Acids Research 17 (1989) 6795-6807
3. Germini A., Zanetti A., Salati C., Rossi S., Forre C., Schmid S., et al. Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. Journal of Agricultural Food Chemistry 52 (2004) 3275-3280
4. Hoofar J., Malorny B., Abdulmawjood A., Cook N., Wagner M., and Fach P. Practical considerations in design of internal amplification controls for diagnostic PCR assays. Journal of Clinical Microbiology 42 (2004) 1863-1868
5. Kuribara H., Shindo Y., Matsuoka T., Takubo K., Futo S., Aoki N., et al. Novel reference molecules for quantitation of genetically modified maize and soybean. Journal of AOAC International 85 (2002) 1077-1089
6. Macarthur R., Murray A.W.A., Allnutt T.R., Deppe C., Hird H.J., Kerins G.M., Blackburn J., Brown J., Stones R., and Hugo S. Model for tuning GMO detection in seed and grain. Nature Biotechnology 25 (2007) 169-170
7. Remund K.M., Dixon D.A., Wright D.L., and Holden L.R. Statistical considerations in seed purity testing for transgenic traits. Seed Science Research 11 (2001) 101-119
8. Taverniers I., Windels P., Vaitilingom M., Milcamps A., Van Bockstaele E., Van den Eede G., et al. Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola. Journal of Agricultural Food Chemistry 53 (2005) 3041-3052
9. Trapmann, S., Catalani, P., Conneely, P., Corbisier, P., Gancberg, D., Hannes, E., et al. (2002). The certification of reference materials of dry-mixed soya powder with different mass fractions of Roundup Ready™ soya. European Communities, Luxembourg, EUR 20273 EN, ISBN 92-894-3725-1.
10. Trapmann S., and Emons H. Reliable GMO analysis. Analytical and Bioanalytical Chemistry 381 (2005) 72-74
11. Weighardt F. European GMO labeling thresholds impractical and unscientific. Nature Biotechnology 24 (2006) 23-25
12. Windels P., Taverniers A., Depicker A., Van Bockstaele E., and De Loose M. Characterisation of the Roundup Ready soybean insert. European Food Research Technology 213 (2001) 107-112
13. Yanisch-Perron C., Vieira J., and Messing J. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33 (1985) 103-119