Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification

Analytical Chemistry

Volumn 84, Issue 2, 2012, Pages 1003-1011

  Pinheiro, Leonardo B ^a   Coleman, Victoria A ^a   Hindson, Christopher M ^b   Herrmann, Jan ^a   Hindson, Benjamin J ^b   Bhat, Somanath ^a   Emslie, Kerry R ^a  

^a NATIONAL MEASUREMENT INSTITUTE   (Australia)

^b BIO RAD LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID MOLECULES; COPY NUMBER; DNA CONCENTRATION; DNA MOLECULES; DNA TARGETS; KEY PERFORMANCE PARAMETERS; LOWER DENSITY; MICROFLUIDIC CHAMBERS; ORDERS OF MAGNITUDE; WATER-IN-OIL;

ASSAYS; DNA; DNA SEQUENCES; DROPS; UNCERTAINTY ANALYSIS;

POLYMERASE CHAIN REACTION;

DNA;

ARTICLE; BACTERIOPHAGE LAMBDA; COPY NUMBER VARIATION; EVALUATION; GENETICS; GENOME; HIGH THROUGHPUT SEQUENCING; POLYMERASE CHAIN REACTION;

BACTERIOPHAGE LAMBDA; DNA; DNA COPY NUMBER VARIATIONS; GENOME; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; POLYMERASE CHAIN REACTION;

EID: 84855982119     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac202578x     Document Type: Article

References (15)

1. Sykes, P. J.; Neoh, S. H.; Brisco, M. J.; Hughes, E.; Condon, J.; Morley, A. A. Biotechniques 1992, 13, 444-449
2. Vogelstein, B.; Kinzler, K. W. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 9236-9241
3. Sanders, R.; Huggett, J. F.; Bushell, C. A.; Cowen, S.; Scott, D. J.; Foy, C. A. Anal. Chem. 2011, 83, 6474-6484
4. Lo, Y. M.; Lun, F. M.; Chan, K. C.; Tsui, N. B.; Chong, K. C.; Lau, T. K.; Leung, T. Y.; Zee, B. C.; Cantor, C. R.; Chiu, R. W. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 13116-13121
5. Wang, J.; Ramakrishnan, R.; Tang, Z.; Fan, W.; Kluge, A.; Dowlati, A.; Jones, R. C.; Ma, P. C. Clin. Chem. 2010, 56, 623-632
6. Dube, S.; Qin, J.; Ramakrishnan, R. PLoS One 2008, 3, e2876
7. Bhat, S.; Herrmann, J.; Armishaw, P.; Corbisier, P.; Emslie, K. R. Anal. Bioanal. Chem. 2009, 394, 457-467
8. Hindson, B. J.; Ness, K. D.; Masquelier, D. A.; Belgrader, P.; Heredia, N. J.; Makarewicz, A. J.; Bright, I. J.; Lucero, M. Y.; Hiddessen, A. L.; Legler, T. C.; Kitano, T. K.; Hodel, M. R.; Petersen, J. F.; Wyatt, P. W.; Steenblock, E. R.; Shah, P. H.; Bousse, L. J.; Troup, C. B.; Mellen, J. C.; Wittmann, D. K.; Erndt, N. G.; Cauley, T. H.; Koehler, R. T.; So, A. P.; Dube, S.; Rose, K. A.; Montesclaros, L.; Wang, S.; Stumbo, D. P.; Hodges, S. P.; Romine, S.; Milanovich, F. P.; White, H. E.; Regan, J. F.; Karlin-Neumann, G. A.; Hindson, C. M.; Saxonov, S.; Colston, B. W. Anal. Chem. 2011, 83, 8604-8610
9. Bhat, S.; Curach, N.; Mostyn, T.; Bains, G. S.; Griffiths, K. R.; Emslie, K. R. Anal. Chem. 2010, 82, 7185-7192
10. Weaver, S.; Dube, S.; Mir, A.; Qin, J.; Sun, G.; Ramakrishnan, R.; Jones, R. C.; Livak, K. J. Methods 2010, 50, 271-276
11. Abramoff, M. D.; Magalhaes, P. J.; Ram, S. J. Biophoton. Int. 2004, 11, 36-42
12. Joint Committee for Guides in Metrology Working Group 2. International Vocabulary of Metrology-Basic and general concepts and associated terms (VIM); http://www.bipm.org/utils/common/documents/jcgm/JCGM-200-2008.pdf, 3 rd ed.; BIPM: Sèvres Cedex, France, 2008.
13. Heyries, K. A.; Tropini, C.; Vaninsberghe, M.; Doolin, C.; Petriv, O. I.; Singhal, A.; Leung, K.; Hughesman, C. B.; Hansen, C. L. Nat. Methods 2011, 8, 649-651
14. Pekin, D.; Skhiri, Y.; Baret, J. C.; Le Corre, D.; Mazutis, L.; Salem, C. B.; Millot, F.; El Harrak, A.; Hutchison, J. B.; Larson, J. W.; Link, D. R.; Laurent-Puig, P.; Griffiths, A. D.; Taly, V. Lab Chip 2011, 11, 2156-2166
15. Griffiths, K. R.; Burke, D. G.; Emslie, K. R. Anal. Methods 2011, 3, 2201-2211