iAID: An improved auxin-inducible degron system for the construction of a 'tight' conditional mutant in the budding yeast Saccharomyces cerevisiae

Yeast

Volumn 32, Issue 8, 2015, Pages 567-581

  Tanaka, Seiji ^a,b   Miyazawa Onami, Mayumi ^a   Iida, Tetsushi ^a,b   Araki, Hiroyuki ^a,b  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b GRADUATE UNIVERSITY FOR ADVANCED STUDIES   (Japan)

Author keywords

Auxin inducible degron; Conditional mutant; Dpb11; Mcm10; S. cerevisiae

Indexed keywords

AUXIN; CYCLIN DEPENDENT KINASE; DNA FRAGMENT; DOXYCYCLINE; FUNGAL PROTEIN; MINICHROMOSOME MAINTENANCE PROTEIN 4; TETRACYCLINE; INDOLEACETIC ACID DERIVATIVE; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CELL CYCLE M PHASE; CELL CYCLE PROGRESSION; CELL CYCLE S PHASE; CELL GROWTH; CONCENTRATION (PARAMETERS); DNA CONTENT; DNA REPLICATION; DOWN REGULATION; ENZYME ACTIVATION; FUNGAL GENE; FUNGAL GENETICS; FUNGAL STRAIN; FUNGUS GROWTH; G1 PHASE CELL CYCLE CHECKPOINT; GENE CASSETTE; GENE EXPRESSION; GENE FUNCTION; GENE ISOLATION; GENE LOCUS; GENE MUTATION; GENE REPLICATION; GENE REPRESSION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; GROWTH INHIBITION; IMPROVED AUXIN INDUCIBLE DEGRON SYSTEM; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; DRUG EFFECTS; EVALUATION STUDY; GENE TARGETING; GENETICS; MUTAGENESIS; MUTATION; PROCEDURES;

EUKARYOTA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

GENE TARGETING; INDOLEACETIC ACIDS; MUTAGENESIS; MUTATION; PROMOTER REGIONS, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84938950931     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.3080     Document Type: Article

References (25)

1. Araki H, Leem SH, Phongdara A, Sugino A. 1995. Dpb11, which interacts with DNA polymerase II(ε) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc Natl Acad Sci U S A 92: 11791-11795.
2. Bahler J, Wu JQ, Longtine MS, et al. 1998. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951.
3. Belli G, Gari E, Piedrafita L, et al. 1998. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res 26: 942-947.
4. Dohmen RJ, Wu P, Varshavsky A. 1994. Heat-inducible degron: a method for constructing temperature-sensitive mutants. Science 263: 1273-1276.
5. Goldstein AL, McCusker JH. 1999. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541-1553.
6. Gossen M, Bujard H. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A 89: 5547-5551.
7. Guldener U, Heck S, Fielder T, et al. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524.
8. Kanke M, Nishimura K, Kanemaki M, et al. 2011. Auxin-inducible protein depletion system in fission yeast. BMC Cell Biol 12: 8.
9. Kubota T, Nishimura K, Kanemaki MT, Donaldson AD. 2013. The Elg1 replication factor C-like complex functions in PCNA unloading during DNA replication. Mol Cell 50: 273-280.
10. 1-phase and are required to establish, but not maintain, the S-phase checkpoint. Mol Biol Cell 12: 3658-3667.
11. Lindner K, Gregan J, Montgomery S, Kearsey SE. 2002. Essential role of MCM proteins in premeiotic DNA replication. Mol Biol Cell 13: 435-444.
12. Longtine MS, McKenzie A III, Demarini DJ, et al. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961.
13. Masai H, Matsumoto S, You Z, et al. 2010. Eukaryotic chromosome DNA replication: where, when, and how? Annu Rev Biochem 79: 89-130.
14. Mnaimneh S, Davierwala AP, Haynes J, et al. 2004. Exploration of essential gene functions via titratable promoter alleles. Cell 118: 31-44.
15. Morawska M, Ulrich HD. 2013. An expanded tool kit for the auxin-inducible degron system in budding yeast. Yeast 30: 341-351.
16. Nishimura K, Fukagawa T, Takisawa H, et al. 2009. An auxin-based degron system for the rapid depletion of proteins in non-plant cells. Nat Methods 6: 917-922.
17. Tanaka S, Araki H. 2013. Helicase activation and establishment of replication forks at chromosomal origins of replication. Cold Spring Harb Perspect Biol 5: a010371.
18. 1 phase. Nat Cell Biol 4: 198-207.
19. Tanaka S, Komeda Y, Umemori T, et al. 2013. Efficient initiation of DNA replication in eukaryotes requires Dpb11/TopBP1-GINS interaction. Mol Cell Biol 33: 2614-2622.
20. Tanaka S, Nakato R, Katou Y, et al. 2011. Origin association of sld3, sld7, and cdc45 proteins is a key step for determination of origin-firing timing. Curr Biol 21: 2055-2063.
21. Tanaka S, Umemori T, Hirai K, et al. 2007. CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445: 328-332.
22. van Deursen F, Sengupta S, De Piccoli G, et al. 2012. Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. EMBO J 31: 2195-2206.
23. Watase G, Takisawa H, Kanemaki MT. 2012. Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS. Curr Biol 22: 343-349.
24. Xu Z, Wei W, Gagneur J, et al. 2009. Bidirectional promoters generate pervasive transcription in yeast. Nature 457: 1033-1037.
25. Zilio N, Wehrkamp-Richter S, Boddy MN. 2012. A new versatile system for rapid control of gene expression in the fission yeast Schizosaccharomyces pombe. Yeast 29: 425-434.