Small-Molecule-Driven Direct Reprogramming of Mouse Fibroblasts into Functional Neurons

Cell Stem Cell

Volumn 17, Issue 2, 2015, Pages 195-203

  Li, Xiang ^a,b   Zuo, Xiaohan ^c   Jing, Junzhan ^c   Ma, Yantao ^a,b,c   Wang, Jiaming ^a,b   Liu, Defang ^c   Zhu, Jialiang ^a,b   Du, Xiaomin ^a,b   Xiong, Liang ^a,b,c   Du, Yuanyuan ^a,b   Xu, Jun ^a,b   Xiao, Xiong ^c   Wang, Jinlin ^c   Chai, Zhen ^c   Zhao, Yang ^a,b   Deng, Hongkui ^a,b,c  

^a Peking University   (China)

^b PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

^c PEKING UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID; 4 [4 (1,3 BENZODIOXOL 5 YL) 5 (2 PYRIDINYL) 1H IMIDAZOL 2 YL]BENZAMIDE; 6 [[2 [[4 (2,4 DICHLOROPHENYL) 5 (4 METHYL 1H IMIDAZOL 2 YL) 2 PYRIMIDINYL]AMINO]ETHYL]AMINO]NICOTINONITRILE; 7 (3,5 DIMETHYL 4 ISOXAZOLYL) 1,3 DIHYDRO 8 METHOXY 1 [1 (2 PYRIDINYL)ETHYL] 2H IMIDAZO[4,5 C]QUINOLIN 2 ONE; BROMODOMAIN INHIBITOR; BROXURIDINE; FORSKOLIN; MICROTUBULE ASSOCIATED PROTEIN 2; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR BRN2; TRANSCRIPTION FACTOR MASH1; TRANSCRIPTION FACTOR MYT1L; UNCLASSIFIED DRUG; VESICULAR GLUTAMATE TRANSPORTER 1; FUSED HETEROCYCLIC RINGS; MOLECULAR LIBRARY;

ACTION POTENTIAL; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL FATE; CELL FUNCTION; CELL LINEAGE; CELL MATURATION; CELL POPULATION; CONTROLLED STUDY; ELECTROPHYSIOLOGY; EXCITATORY POSTSYNAPTIC POTENTIAL; FIBROBLAST; GENE EXPRESSION PROFILING; GENETIC MANIPULATION; GERM LAYER; ION TRANSPORT; MOLECULE; MOUSE; NERVE CELL; NERVE CELL DIFFERENTIATION; NERVE FIBER GROWTH; NERVOUS SYSTEM DEVELOPMENT; NEURITE; NONHUMAN; NUCLEAR REPROGRAMMING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RANDOMIZED CONTROLLED TRIAL; REGENERATIVE MEDICINE; SMALL MOLECULE; SOMATIC CELL; SYNAPTIC TRANSMISSION; TRANSGENE; ANIMAL; CELL PROLIFERATION; CYTOLOGY; DRUG EFFECTS; GENETICS; METABOLISM; MOLECULAR LIBRARY; PHARMACOLOGY;

ANIMALS; CELL LINEAGE; CELL PROLIFERATION; CELLULAR REPROGRAMMING; ELECTROPHYSIOLOGICAL PHENOMENA; FIBROBLASTS; GENE EXPRESSION PROFILING; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; MICE; NEURONS; SMALL MOLECULE LIBRARIES; TRANSCRIPTION FACTORS;

EID: 84938692220     PISSN: 19345909     EISSN: 18759777     Source Type: Journal    
DOI: 10.1016/j.stem.2015.06.003     Document Type: Article

References (28)

1. N. Bertrand, D.S. Castro, and F. Guillemot Proneural genes and the specification of neural cell types Nat. Rev. Neurosci. 3 2002 517 530
2. N.A. Bhowmick, A. Chytil, D. Plieth, A.E. Gorska, N. Dumont, S. Shappell, M.K. Washington, E.G. Neilson, and H.L. Moses TGF-beta signaling in fibroblasts modulates the oncogenic potential of adjacent epithelia Science 303 2004 848 851
3. M. Caiazzo, M.T. Dell'Anno, E. Dvoretskova, D. Lazarevic, S. Taverna, D. Leo, T.D. Sotnikova, A. Menegon, P. Roncaglia, G. Colciago, and et al. Direct generation of functional dopaminergic neurons from mouse and human fibroblasts Nature 476 2011 224 227
4. S.M. Chambers, C.A. Fasano, E.P. Papapetrou, M. Tomishima, M. Sadelain, and L. Studer Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling Nat. Biotechnol. 27 2009 275 280
5. S. Chanda, C.E. Ang, J. Davila, C. Pak, M. Mall, Q.Y. Lee, H. Ahlenius, S.W. Jung, T.C. Südhof, and M. Wernig Generation of induced neuronal cells by the single reprogramming factor ASCL1 Stem Cell Reports 3 2014 282 296
6. C.M. Chiang Nonequivalent response to bromodomain-targeting BET inhibitors in oligodendrocyte cell fate decision Chem. Biol. 21 2014 804 806
7. R.L. Davis, H. Weintraub, and A.B. Lassar Expression of a single transfected cDNA converts fibroblasts to myoblasts Cell 51 1987 987 1000
8. R. Di Micco, B. Fontanals-Cirera, V. Low, P. Ntziachristos, S.K. Yuen, C.D. Lovell, I. Dolgalev, Y. Yonekubo, G. Zhang, E. Rusinova, and et al. Control of embryonic stem cell identity by BRD4-dependent transcriptional elongation of super-enhancer-associated pluripotency genes Cell Rep. 9 2014 234 247
9. Y. Du, J. Wang, J. Jia, N. Song, C. Xiang, J. Xu, Z. Hou, X. Su, B. Liu, T. Jiang, and et al. Human hepatocytes with drug metabolic function induced from fibroblasts by lineage reprogramming Cell Stem Cell 14 2014 394 403
10. S. Dworkin, and T. Mantamadiotis Targeting CREB signalling in neurogenesis Expert Opin. Ther. Targets 14 2010 869 879
11. J. Hanna, S. Markoulaki, P. Schorderet, B.W. Carey, C. Beard, M. Wernig, M.P. Creyghton, E.J. Steine, J.P. Cassady, R. Foreman, and et al. Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency Cell 133 2008 250 264
12. P. Hou, Y. Li, X. Zhang, C. Liu, J. Guan, H. Li, T. Zhao, J. Ye, W. Yang, K. Liu, and et al. Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds Science 341 2013 651 654
13. M. Iwano, D. Plieth, T.M. Danoff, C. Xue, H. Okada, and E.G. Neilson Evidence that fibroblasts derive from epithelium during tissue fibrosis J. Clin. Invest. 110 2002 341 350
14. J. Kim, S.C. Su, H. Wang, A.W. Cheng, J.P. Cassady, M.A. Lodato, C.J. Lengner, C.Y. Chung, M.M. Dawlaty, L.H. Tsai, and R. Jaenisch Functional integration of dopaminergic neurons directly converted from mouse fibroblasts Cell Stem Cell 9 2011 413 419
15. J. Ladewig, J. Mertens, J. Kesavan, J. Doerr, D. Poppe, F. Glaue, S. Herms, P. Wernet, G. Kögler, F.J. Müller, and et al. Small molecules enable highly efficient neuronal conversion of human fibroblasts Nat. Methods 9 2012 575 578
16. M.L. Liu, T. Zang, Y. Zou, J.C. Chang, J.R. Gibson, K.M. Huber, and C.L. Zhang Small molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons Nat. Commun. 4 2013 2183
17. L. Madisen, T.A. Zwingman, S.M. Sunkin, S.W. Oh, H.A. Zariwala, H. Gu, L.L. Ng, R.D. Palmiter, M.J. Hawrylycz, A.R. Jones, and et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain Nat. Neurosci. 13 2010 133 140
18. J.W. Schneider, Z. Gao, S. Li, M. Farooqi, T.S. Tang, I. Bezprozvanny, D.E. Frantz, and J. Hsieh Small-molecule activation of neuronal cell fate Nat. Chem. Biol. 4 2008 408 410
19. J. Seal, Y. Lamotte, F. Donche, A. Bouillot, O. Mirguet, F. Gellibert, E. Nicodeme, G. Krysa, J. Kirilovsky, S. Beinke, and et al. Identification of a novel series of BET family bromodomain inhibitors: binding mode and profile of I-BET151 (GSK1210151A) Bioorg. Med. Chem. Lett. 22 2012 2968 2972
20. K.B. Seamon, W. Padgett, and J.W. Daly Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells Proc. Natl. Acad. Sci. USA 78 1981 3363 3367
21. F. Strutz, H. Okada, C.W. Lo, T. Danoff, R.L. Carone, J.E. Tomaszewski, and E.G. Neilson Identification and characterization of a fibroblast marker: FSP1 J. Cell Biol. 130 1995 393 405
22. K.L. Tucker, M. Meyer, and Y.A. Barde Neurotrophins are required for nerve growth during development Nat. Neurosci. 4 2001 29 37
23. T. Vierbuchen, A. Ostermeier, Z.P. Pang, Y. Kokubu, T.C. Südhof, and M. Wernig Direct conversion of fibroblasts to functional neurons by defined factors Nature 463 2010 1035 1041
24. J. Wang, K. Zhang, L. Xu, and E. Wang Quantifying the Waddington landscape and biological paths for development and differentiation Proc. Natl. Acad. Sci. USA 108 2011 8257 8262
25. T. Wu, H.B. Pinto, Y.F. Kamikawa, and M.E. Donohoe The BET family member BRD4 interacts with OCT4 and regulates pluripotency gene expression Stem Cell Reports 4 2015 390 403 10.1016/j.stemcr.2015.01.012
26. J. Xu, Y. Du, and H. Deng Direct lineage reprogramming: strategies, mechanisms, and applications Cell Stem Cell 16 2015 119 134
27. A.S. Yoo, A.X. Sun, L. Li, A. Shcheglovitov, T. Portmann, Y. Li, C. Lee-Messer, R.E. Dolmetsch, R.W. Tsien, and G.R. Crabtree MicroRNA-mediated conversion of human fibroblasts to neurons Nature 476 2011 228 231
28. C. Yu, K. Liu, S. Tang, and S. Ding Chemical approaches to cell reprogramming Curr. Opin. Genet. Dev. 28 2014 50 56