Hot fusion: An efficient method to clone multiple DNA fragments as well as inverted repeats without ligase

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Fu, Changlin ^a   Donovan, William P ^a   Shikapwashya Hasser, Olga ^a   Ye, Xudong ^a   Cole, Robert H ^a  

^a MONSANTO COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; LIGASE; PLASMID VECTOR; DNA;

ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; ESCHERICHIA COLI; HOT FUSION; INTERMETHOD COMPARISON; INVERTED REPEAT; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION SITE; RNA INTERFERENCE; ROOM TEMPERATURE; STRUCTURAL HOMOLOGY; CHEMISTRY; GENE VECTOR; GENETICS; METABOLISM; MUTAGENESIS; PLANT GENE; PLASMID; PROCEDURES; TIME FACTOR;

CLONING, MOLECULAR; DNA; ESCHERICHIA COLI; GENES, PLANT; GENETIC VECTORS; INVERTED REPEAT SEQUENCES; LIGASES; MUTAGENESIS; PLASMIDS; RNA INTERFERENCE; TIME FACTORS;

EID: 84938634570     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0115318     Document Type: Article

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