High-throughput construction of intron-containing hairpin RNA vectors for RNAi in plants

PLoS ONE

Volumn 7, Issue 5, 2012, Pages

  Yan, Pu ^a,b,d   Shen, Wentao ^b   Gao, XinZheng ^c   Li, Xiaoying ^b   Zhou, Peng ^b   Duan, Jun ^a  

^a SOUTH CHINA INSTITUTE OF BOTANY   (China)

^b INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY   (China)

^c HAINAN MEDICAL UNIVERSITY   (China)

^d UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN BSAI; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG; PLANT RNA; SMALL INTERFERING RNA;

AMINO ACID SEQUENCE; ANTISENSE VECTOR; ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; FUNCTIONAL GENOMICS; GENE SILENCING; GENE STRUCTURE; GENETIC ANALYSIS; GOLDEN GATE CLONING; INTRON CONTAINING HAIRPIN RNA VECTOR; MARKER GENE; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENETICS; POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA VECTOR; GENE ORDER; GENE VECTOR; GENETICS; GENOMICS; INTRON; METHODOLOGY; PLANT; PLANT GENE; REPORTER GENE;

CLONING, MOLECULAR; GENE ORDER; GENE SILENCING; GENES, PLANT; GENES, REPORTER; GENETIC VECTORS; GENOMICS; INTRONS; PLANTS; RNA INTERFERENCE; RNA, PLANT; RNA, SMALL INTERFERING;

EID: 84861657228     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038186     Document Type: Article

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