One-step sequence-and ligation-independent cloning as a rapid and versatile cloning method for functional genomics Studies

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5440-5443

  Jeong, Jae Yeon ^a   Yim, Hyung Soon ^a   Ryu, Ji Young ^a   Lee, Hyun Sook ^a   Lee, Jung Hyun ^a   Seen, Dong Seung ^b   Kang, Sung Gyun ^a  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

^b Macrogen Inc   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TRANSFORMATION; DIRECTIONAL CLONING; DNA POLYMERASE; FUNCTIONAL GENOMICS; ROOM TEMPERATURE;

POLYMERASE CHAIN REACTION;

CLONING;

DNA DIRECTED DNA POLYMERASE; GENE 43 PROTEIN, ENTEROBACTERIA PHAGE T4; VIRUS PROTEIN;

BACTERIOLOGY; BACTERIUM; COST-BENEFIT ANALYSIS; GENOMICS; LIGAND; MICROBIAL ACTIVITY; MOLECULAR ANALYSIS; TEMPERATURE EFFECT;

ARTICLE; BACTERIUM TRANSFORMATION; GENE VECTOR; GENETICS; GENOMICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; CLONING, MOLECULAR; DNA-DIRECTED DNA POLYMERASE; GENETIC VECTORS; GENOMICS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; TRANSFORMATION, BACTERIAL; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 84866171028     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00844-12     Document Type: Article

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