13C-based metabolic flux analysis of Saccharomyces cerevisiae with a reduced Crabtree effect

Journal of Bioscience and Bioengineering

Volumn 120, Issue 2, 2015, Pages 140-144

  Kajihata, Shuichi ^a   Matsuda, Fumio ^a   Yoshimi, Mika ^a   Hayakawa, Kenshi ^a   Furusawa, Chikara ^a,b   Kanda, Akihisa ^c   Shimizu, Hiroshi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b RIKEN Center for Biosystems Dynamics Research   (Japan)

^c KANEKA CORPORATION   (Japan)

Author keywords

13C based metabolic flux analysis; Central carbon metabolism; Crabtree effect; Redox balance; Saccharomyces cerevisiae

Indexed keywords

CHEMOSTATS; ETHANOL; GLUCOSE; METABOLISM;

AEROBIC CONDITION; CENTRAL CARBON METABOLISMS; CRABTREE EFFECT; FED-BATCH CULTIVATION; METABOLIC FLUX ANALYSIS; METABOLIC FLUX DISTRIBUTION; PENTOSE PHOSPHATE PATHWAY; REDOX BALANCES;

YEAST;

ACETYL COENZYME A; ADENOSINE TRIPHOSPHATE; CARBON 13; GLUCOSE 6 PHOSPHATE; GLYCERALDEHYDE 3 PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; ALCOHOL; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVIC ACID;

ARTICLE; BIOSYNTHESIS; CARBON SOURCE; CELL GROWTH; CHEMOSTAT; CRABTREE EFFECT; CYTOSOL; FUNGAL BIOMASS; FUNGAL CELL; FUNGAL METABOLISM; FUNGAL PHENOMENA AND FUNCTIONS; GLUCOSE INTAKE; METABOLIC FLUX ANALYSIS; METABOLIC REGULATION; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PENTOSE PHOSPHATE CYCLE; PROTEIN METABOLISM; RESPIRATORY CHAIN; SACCHAROMYCES CEREVISIAE; STEADY STATE; AEROBIC METABOLISM; BIOMASS; CYTOLOGY; GLYCOLYSIS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; OXIDATION REDUCTION REACTION;

SACCHAROMYCES CEREVISIAE;

ACETYL COENZYME A; AEROBIOSIS; BIOMASS; ETHANOL; GLUCOSE; GLYCOLYSIS; METABOLIC FLUX ANALYSIS; NADP; OXIDATION-REDUCTION; PENTOSE PHOSPHATE PATHWAY; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVIC ACID; SACCHAROMYCES CEREVISIAE;

EID: 84937251082     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2014.12.014     Document Type: Article

References (27)

1. De Deken R.H. The Crabtree effect: a regulatory system in yeast. J. Gen. Microbiol. 1966, 44:149-156.
2. 13C flux analysis. Microb. Cell. Fact. 2005, 4:30.
3. Vemuri G.N., Eiteman M.A., McEwen J.E., Olsson L., Nielsen J. Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 2007, 104:2402-2407.
4. Diaz-Ruiz R., Averet N., Araiza D., Pinson B., Uribe-Carvajal S., Devin A., Rigoulet M. Mitochondrial oxidative phosphorylation is regulated by fructose 1,6-bisphosphate. A possible role in Crabtree effect induction?. J. Biol. Chem. 2008, 283:26948-26955.
5. Kresze G.B., Ronft H. Pyruvate dehydrogenase complex from baker's yeast. 1. Purification and some kinetic and regulatory properties. Eur. J. Biochem. 1981, 119:573-579.
6. Pronk J.T., Yde Steensma H., Van Dijken J.P. Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 1996, 12:1607-1633.
7. Boiteux A., Hess B. Allosteric properties of yeast pyruvate decarboxylase. FEBS Lett. 1970, 9:293-296.
8. Gombert A.K., Moreira dos Santos M., Christensen B., Nielsen J. Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression. J. Bacteriol. 2001, 183:1441-1451.
9. Brachmann C.B., Davies A., Cost G.J., Caputo E., Li J., Hieter P., Boeke J.D. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 1998, 14:115-132.
10. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 1983, 153:163-168.
11. Okahashi N., Kajihata S., Furusawa C., Shimizu H. Reliable metabolic flux estimation in Escherichia coli central carbon metabolism using intracellular free amino acids. Metabolites 2014, 4:408-420.
12. 13C enrichment data of free amino acids for precise metabolic flux analysis. Biotechnol. J. 2011, 6:1377-1387.
13. 13C-labeled substrates. Eukaryot. Cell 2003, 2:599-608.
14. Monschau N., Stahmann K.P., Sahm H., McNeil J.B., Bognar A.L. Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis. FEMS Microbiol. Lett. 1997, 150:55-60.
15. Forster J., Famili I., Fu P., Palsson B.O., Nielsen J. Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network. Genome Res. 2003, 13:244-253.
16. 13C-based metabolic flux analysis. BioMed Res. Int. 2014, 2014:627014.
17. 13C-labeled metabolic flux analysis of a fed-batch culture of elutriated Saccharomyces cerevisiae. FEMS Yeast Res. 2007, 7:511-526.
18. Antoniewicz M.R., Kelleher J.K., Stephanopoulos G. Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements. Metab. Eng. 2006, 8:324-337.
19. +. FEBS Lett. 1989, 254:199-202.
20. Bruinenberg P.M., Waslander G.W., van Dijken J.P., Scheffers W.A. A comparative radiorespirometric study of glucose metabolism in yeasts. Yeast 1986, 2:117-121.
21. Kummel A., Ewald J.C., Fendt S.M., Jol S.J., Picotti P., Aebersold R., Sauer U., Zamboni N., Heinemann M. Differential glucose repression in common yeast strains in response to HXK2 deletion. FEMS Yeast Res. 2010, 10:322-332.
22. Sollner S., Deller S., Macheroux P., Palfey B.A. Mechanism of flavin reduction and oxidation in the redox-sensing quinone reductase Lot6p from Saccharomyces cerevisiae. Biochemistry 2009, 48:8636-8643.
23. 13C flux analysis and metabolomics. FEMS Yeast Res. 2011, 11:263-272.
24. Larsson C., Pahlman I.L., Ansell R., Rigoulet M., Adler L., Gustafsson L. The importance of the glycerol 3-phosphate shuttle during aerobic growth of Saccharomyces cerevisiae. Yeast 1998, 14:347-357.
25. Rigoulet M., Aguilaniu H., Averet N., Bunoust O., Camougrand N., Grandier-Vazeille X., Larsson C., Pahlman I.L., Manon S., Gustafsson L. Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae. Mol. Cell Biochem. 2004, 256-257:73-81.
26. 13C-labeling experiments prove important differences in protein turnover rate between two Saccharomyces cerevisiae strains. FEMS Yeast Res. 2012, 12:741-747.
27. Lahtvee P.J., Seiman A., Arike L., Adamberg K., Vilu R. Protein turnover forms one of the highest maintenance costs in Lactococcus lactis. Microbiology 2014, 160:1501-1512.