메뉴 건너뛰기




Volumn 7, Issue 4, 2007, Pages 511-526

13C-Labeled metabolic flux analysis of a fed-batch culture of elutriated Saccharomyces cerevisiae

Author keywords

13C labeling experiment; Cell division cycle; LC MS analysis; Metabolic flux analysis; Saccharomyces cerevisiae; Synchronized culture

Indexed keywords

CARBON; CARBON 13; PENTOSE PHOSPHATE;

EID: 34249007773     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2006.00199.x     Document Type: Article
Times cited : (32)

References (56)
  • 1
    • 33745155105 scopus 로고    scopus 로고
    • Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements
    • Antoniewicz MR, Kelleher JK Stephanopoulos G (2006) Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements. Metabol Eng 8: 324 337.
    • (2006) Metabol Eng , vol.8 , pp. 324-337
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 2
    • 0030804853 scopus 로고    scopus 로고
    • Flux analysis of underdetermined metabolic systems: The quest for missing constraints
    • Bonarius HPJ, Schmidt G Tramper J (1997) Flux analysis of underdetermined metabolic systems: the quest for missing constraints. Trends Biotechnol 15: 308 314.
    • (1997) Trends Biotechnol , vol.15 , pp. 308-314
    • Bonarius, H.P.J.1    Schmidt, G.2    Tramper, J.3
  • 3
    • 0034787569 scopus 로고    scopus 로고
    • Participation of acetaldehyde dehydrogenases in ethanol and pyruvate metabolism of the yeast Saccharomyces cerevisiae
    • Boubekeur S, Camougrand N, Bunoust O, Rigoulet M Guérin B (2001) Participation of acetaldehyde dehydrogenases in ethanol and pyruvate metabolism of the yeast Saccharomyces cerevisiae. Eur J Biochem 268: 5057 5065.
    • (2001) Eur J Biochem , vol.268 , pp. 5057-5065
    • Boubekeur, S.1    Camougrand, N.2    Bunoust, O.3    Rigoulet, M.4    Guérin, B.5
  • 4
    • 0033205580 scopus 로고    scopus 로고
    • Isotopomer analysis using GC-MS
    • Christensen B Nielsen J (2000) Isotopomer analysis using GC-MS. Metabol Eng 1: 282 290.
    • (2000) Metabol Eng , vol.1 , pp. 282-290
    • Christensen, B.1    Nielsen, J.2
  • 5
    • 0036271587 scopus 로고    scopus 로고
    • Analysis of flux estimates based on 13C-labelling experiments
    • Christensen B, Gombert AK Nielsen J (2002) Analysis of flux estimates based on 13C-labelling experiments. Eur J Biochem 269: 2795 2800.
    • (2002) Eur J Biochem , vol.269 , pp. 2795-2800
    • Christensen, B.1    Gombert, A.K.2    Nielsen, J.3
  • 6
    • 0018360052 scopus 로고
    • Reduction of perturbations in leucine incorporation in synchronous cultures of Schizosaccharomyces pombe made by elutriation
    • Creanor J Mitchison JM (1979) Reduction of perturbations in leucine incorporation in synchronous cultures of Schizosaccharomyces pombe made by elutriation. J Gen Microbiol 112: 385 388.
    • (1979) J Gen Microbiol , vol.112 , pp. 385-388
    • Creanor, J.1    Mitchison, J.M.2
  • 7
    • 0030592434 scopus 로고    scopus 로고
    • Physiology of Saccharomyces cerevisiae during cell cycle oscillations
    • Duboc P, Marison I von Stockar U (1996) Physiology of Saccharomyces cerevisiae during cell cycle oscillations. J Biotechnol 51: 57 72.
    • (1996) J Biotechnol , vol.51 , pp. 57-72
    • Duboc, P.1    Marison, I.2    Von Stockar, U.3
  • 9
    • 0032767911 scopus 로고    scopus 로고
    • Cell cycle synchronization
    • Futcher B (1999) Cell cycle synchronization. Methods Cell Science 21: 79 86.
    • (1999) Methods Cell Science , vol.21 , pp. 79-86
    • Futcher, B.1
  • 10
    • 33748430396 scopus 로고    scopus 로고
    • Metabolic cycle, cell cycle, and the finishing kick to Start
    • Futcher B (2006) Metabolic cycle, cell cycle, and the finishing kick to Start. Geno Biol 7 (107 1 5.
    • (2006) Geno Biol , vol.7 , Issue.107 , pp. 1-5
    • Futcher, B.1
  • 11
    • 0035140099 scopus 로고    scopus 로고
    • Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression
    • Gombert AK, Moreiro dos Santos M, Christensen B Nielsen J (2001) Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression. J Bacteriol 183: 1441 1451.
    • (2001) J Bacteriol , vol.183 , pp. 1441-1451
    • Gombert, A.K.1    Moreiro Dos Santos, M.2    Christensen, B.3    Nielsen, J.4
  • 12
    • 1842473645 scopus 로고    scopus 로고
    • Impact of transamination reations and protein turnover on labeling dynamics in 13C-labeling experiments
    • Grotkjaer T, Åkesson M, Christensen B, Gombert AK Nielsen J (2004) Impact of transamination reations and protein turnover on labeling dynamics in 13C-labeling experiments. Biotechnol Bioeng 86: 209 216.
    • (2004) Biotechnol Bioeng , vol.86 , pp. 209-216
    • Grotkjaer, T.1    Åkesson, M.2    Christensen, B.3    Gombert, A.K.4    Nielsen, J.5
  • 13
    • 0037457491 scopus 로고    scopus 로고
    • Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae
    • Hans MA, Heinzle E Wittmann C (2003) Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae. Biotechnol Bioeng 82: 143 151.
    • (2003) Biotechnol Bioeng , vol.82 , pp. 143-151
    • Hans, M.A.1    Heinzle, E.2    Wittmann, C.3
  • 14
    • 10344222155 scopus 로고    scopus 로고
    • How cells coordinate growth and division
    • Jorgensen P Tyers M (2004) How cells coordinate growth and division. Curr Biol 14: R1014 R1027.
    • (2004) Curr Biol , vol.14
    • Jorgensen, P.1    Tyers, M.2
  • 15
    • 0038166873 scopus 로고
    • The purification and properties of trehalase
    • Kalf GF Rieder SV (1958) The purification and properties of trehalase. J Biol Chem 230: 691 698.
    • (1958) J Biol Chem , vol.230 , pp. 691-698
    • Kalf, G.F.1    Rieder, S.V.2
  • 16
    • 26244452731 scopus 로고    scopus 로고
    • Revisiting the 13C-label distribution of the non-oxidative branch of the pentose phosphate pathway based upon kinetic and genetic evidence
    • Kleijn RJ, van Winden WA, van Gulik WM Heijnen JJ (2005) Revisiting the 13C-label distribution of the non-oxidative branch of the pentose phosphate pathway based upon kinetic and genetic evidence. FEBS J 272: 4970 4982.
    • (2005) FEBS J , vol.272 , pp. 4970-4982
    • Kleijn, R.J.1    Van Winden, W.A.2    Van Gulik, W.M.3    Heijnen, J.J.4
  • 17
    • 33746064096 scopus 로고    scopus 로고
    • 13C-labeled gluconate tracing as a direct and accurate method for determining the pentose phosphate pathway split ratio in Penicillium chrysogenum
    • Kleijn RJ, van Winden WA, Ras C, van Gulik WM, Schipper D Heijnen JJ (2006) 13C-labeled gluconate tracing as a direct and accurate method for determining the pentose phosphate pathway split ratio in Penicillium chrysogenum. Appl Environ Microbiol 72: 4743 4754.
    • (2006) Appl Environ Microbiol , vol.72 , pp. 4743-4754
    • Kleijn, R.J.1    Van Winden, W.A.2    Ras, C.3    Van Gulik, W.M.4    Schipper, D.5    Heijnen, J.J.6
  • 18
    • 0842342616 scopus 로고    scopus 로고
    • A genome wide oscillation in transcription gates DNA replication and cell cycle
    • Klevecz RR, Bolen J, Forrest G Murray DB (2004) A genome wide oscillation in transcription gates DNA replication and cell cycle. Proc Natl Acad Sci USA 101: 1200 1205.
    • (2004) Proc Natl Acad Sci USA , vol.101 , pp. 1200-1205
    • Klevecz, R.R.1    Bolen, J.2    Forrest, G.3    Murray, D.B.4
  • 19
    • 0014653793 scopus 로고
    • Changes in carbohydrate composition and trehalase-activity during the budding cycle of Saccharomyces cerevisiae
    • Küenzi M Fiechter A (1969) Changes in carbohydrate composition and trehalase-activity during the budding cycle of Saccharomyces cerevisiae. Arch Microbiol 64: 396 407.
    • (1969) Arch Microbiol , vol.64 , pp. 396-407
    • Küenzi, M.1    Fiechter, A.2
  • 20
    • 34249022963 scopus 로고    scopus 로고
    • Quantitative physiology of Saccharomyces cerevisiae using metabolic network analysis. PhD Thesis, Delft University of Technology, Delft.
    • Lange HC (2002) Quantitative physiology of Saccharomyces cerevisiae using metabolic network analysis. PhD Thesis, Delft University of Technology, Delft.
    • (2002)
    • Lange, H.C.1
  • 21
    • 0035813391 scopus 로고    scopus 로고
    • Statistical reconciliation of the elemental and molecular biomass composition of Saccharomyces cerevisiae
    • Lange HC Heijnen JJ (2001) Statistical reconciliation of the elemental and molecular biomass composition of Saccharomyces cerevisiae. Biotechnol Bioeng 75: 334 344.
    • (2001) Biotechnol Bioeng , vol.75 , pp. 334-344
    • Lange, H.C.1    Heijnen, J.J.2
  • 22
    • 0035014718 scopus 로고    scopus 로고
    • Central carbon metabolism of Saccharomyces cerevisiae explored by biosynthetic fractional 13C-labeling of common amino acids
    • Maaheimo H, Fiaux J, Çakar ZP, Bailey JE, Sauer U Szyperski T (2001) Central carbon metabolism of Saccharomyces cerevisiae explored by biosynthetic fractional 13C-labeling of common amino acids. Eur J Biochem 268: 2464 2479.
    • (2001) Eur J Biochem , vol.268 , pp. 2464-2479
    • Maaheimo, H.1    Fiaux, J.2    Çakar, Z.P.3    Bailey, J.E.4    Sauer, U.5    Szyperski, T.6
  • 23
    • 0030050268 scopus 로고    scopus 로고
    • Determination of fluxes in central metabolism of Corynebacterium glutamicum by NMR spectroscopy combined with metabolite balancing
    • Marx A, de Graaf AA, Wiechert W, Eggeling L Sahm H (1996) Determination of fluxes in central metabolism of Corynebacterium glutamicum by NMR spectroscopy combined with metabolite balancing. Biotechnol Bioeng 49: 111 129.
    • (1996) Biotechnol Bioeng , vol.49 , pp. 111-129
    • Marx, A.1    De Graaf, A.A.2    Wiechert, W.3    Eggeling, L.4    Sahm, H.5
  • 24
    • 1442326111 scopus 로고    scopus 로고
    • MIRACLE: Mass isotopomer ratio analysis of U-13C-labeled extracts. a new method for accurate quantification of changes in concentrations of intracellular metabolites
    • Mashego MR, Wu L, van Dam JC, Ras C, Vinke JL, van Winden WA, van Gulik WM Heijnen JJ (2004) MIRACLE: mass isotopomer ratio analysis of U-13C-labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites. Biotechnol Bioeng 85: 620 628.
    • (2004) Biotechnol Bioeng , vol.85 , pp. 620-628
    • Mashego, M.R.1    Wu, L.2    Van Dam, J.C.3    Ras, C.4    Vinke, J.L.5    Van Winden, W.A.6    Van Gulik, W.M.7    Heijnen, J.J.8
  • 26
    • 34249031801 scopus 로고    scopus 로고
    • Model-assisted analysis of cyclic-AMP signal transduction in Saccharomyces cerevisiae, PhD Thesis, University of Stuttgart, Stuttgart.
    • Müller D (2006) Model-assisted analysis of cyclic-AMP signal transduction in Saccharomyces cerevisiae, PhD Thesis, University of Stuttgart, Stuttgart.
    • (2006)
    • Müller, D.1
  • 27
    • 0037338995 scopus 로고    scopus 로고
    • Cyclic AMP mediates the cell dynamics of energy metabolism in Saccharomyces cerevisiae
    • Müller D, Exler S, Aguilera-Vázquez L, Guerrero-Martín E Reuss M (2003) Cyclic AMP mediates the cell dynamics of energy metabolism in Saccharomyces cerevisiae. Yeast 20: 351 367.
    • (2003) Yeast , vol.20 , pp. 351-367
    • Müller, D.1    Exler, S.2    Aguilera-Vázquez, L.3    Guerrero-Martín, E.4    Reuss, M.5
  • 29
    • 0024278876 scopus 로고
    • Oscillations in continuous cultures of budding yeast: A segregated parameter analysis
    • Porro D, Martegani E, Ranzi BM Alberghina L (1988) Oscillations in continuous cultures of budding yeast: a segregated parameter analysis. Biotechnol Bioeng 32: 411 417.
    • (1988) Biotechnol Bioeng , vol.32 , pp. 411-417
    • Porro, D.1    Martegani, E.2    Ranzi, B.M.3    Alberghina, L.4
  • 30
    • 33646185696 scopus 로고    scopus 로고
    • Genome-wide oscillation of transcription in yeast
    • Reinke H Gatfield P (2006) Genome-wide oscillation of transcription in yeast. Trends Biochem Sci 31: 189 191.
    • (2006) Trends Biochem Sci , vol.31 , pp. 189-191
    • Reinke, H.1    Gatfield, P.2
  • 32
    • 0026594358 scopus 로고
    • Optimal selection of metabolic fluxes for in vivo measurement. I. Development of mathematical methods
    • Savinell JM Palsson BØ (1992) Optimal selection of metabolic fluxes for in vivo measurement. I. Development of mathematical methods. J Theor Biol 155: 201 214.
    • (1992) J Theor Biol , vol.155 , pp. 201-214
    • Savinell, J.M.1    Palsson, BØ.2
  • 33
    • 0343471424 scopus 로고    scopus 로고
    • Modeling isotopomer distributions in biochemical networks using isotopomer mapping matrices
    • Schmidt K, Carlsen M, Nielsen J Villadsen J (1997) Modeling isotopomer distributions in biochemical networks using isotopomer mapping matrices. Biotechnol Bioeng 55: 831 840.
    • (1997) Biotechnol Bioeng , vol.55 , pp. 831-840
    • Schmidt, K.1    Carlsen, M.2    Nielsen, J.3    Villadsen, J.4
  • 36
    • 0000347493 scopus 로고
    • A predictive model for the spontaneous synchronization of Saccharomyces cerevisiae grown in continuous culture. I. Concept
    • Strässle C, Sonnleitner B Fiechter A (1988) A predictive model for the spontaneous synchronization of Saccharomyces cerevisiae grown in continuous culture. I. Concept. J Biotechnol 7: 299 318.
    • (1988) J Biotechnol , vol.7 , pp. 299-318
    • Strässle, C.1    Sonnleitner, B.2    Fiechter, A.3
  • 37
    • 0024606954 scopus 로고
    • A predictive model for the spontaneous synchronisation of Saccharomyces cerevisiae grown in continuous culture. II. Experimental verification
    • Strässle C, Sonnleitner B Fiechter A (1989) A predictive model for the spontaneous synchronisation of Saccharomyces cerevisiae grown in continuous culture. II. Experimental verification. J Biotechnol 9: 191 208.
    • (1989) J Biotechnol , vol.9 , pp. 191-208
    • Strässle, C.1    Sonnleitner, B.2    Fiechter, A.3
  • 38
    • 0030561421 scopus 로고    scopus 로고
    • Detecting and dissecting metabolic fluxes using biosynthetic fractional 13C-labeling and two-dimensional NMR spectroscopy
    • Szyperski T, Bailey JE Wüthrich K (1996) Detecting and dissecting metabolic fluxes using biosynthetic fractional 13C-labeling and two-dimensional NMR spectroscopy. Trends Biotechnol 14: 453 459.
    • (1996) Trends Biotechnol , vol.14 , pp. 453-459
    • Szyperski, T.1    Bailey, J.E.2    Wüthrich, K.3
  • 39
    • 0033857139 scopus 로고    scopus 로고
    • Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes? testing biochemistry
    • Teusink B, Passarge J, Reijenga CA et al 2000) Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes? testing biochemistry. Eur J Biochem 267: 5313 5329.
    • (2000) Eur J Biochem , vol.267 , pp. 5313-5329
    • Teusink, B.1    Passarge, J.2    Reijenga, C.A.3    Al, E.4
  • 40
    • 0027380246 scopus 로고
    • In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique
    • Theobald U, Mailinger W, Reuss M Rizzi M (1993) In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. Anal Biochem 214: 31 37.
    • (1993) Anal Biochem , vol.214 , pp. 31-37
    • Theobald, U.1    Mailinger, W.2    Reuss, M.3    Rizzi, M.4
  • 41
    • 0343471961 scopus 로고    scopus 로고
    • In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae: I. Experimental observations
    • Theobald U, Mailinger W, Baltes M, Rizzi M Reuss M (1997) In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae: I. Experimental observations. Biotechnol Bioeng 55: 305 316.
    • (1997) Biotechnol Bioeng , vol.55 , pp. 305-316
    • Theobald, U.1    Mailinger, W.2    Baltes, M.3    Rizzi, M.4    Reuss, M.5
  • 42
    • 27944487902 scopus 로고    scopus 로고
    • Logic of the yeast metabolic cycle: Temporal compartmentalization of cellular processes
    • Tu BP, Kudlicki A, Rowicka M McKnight SL (2005) Logic of the yeast metabolic cycle: temporal compartmentalization of cellular processes. Science 310: 1152 1158.
    • (2005) Science , vol.310 , pp. 1152-1158
    • Tu, B.P.1    Kudlicki, A.2    Rowicka, M.3    McKnight, S.L.4
  • 43
    • 0027572102 scopus 로고
    • Metabolic flux distribution in Corynebacterium glutamicum during growth and lysine overproduction
    • Vallino JJ Stephanopoulos G (1993) Metabolic flux distribution in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41: 633 646.
    • (1993) Biotechnol Bioeng , vol.41 , pp. 633-646
    • Vallino, J.J.1    Stephanopoulos, G.2
  • 44
    • 0028764649 scopus 로고
    • Linear constraint relations in biochemical reaction systems: II. Diagnosis and estimation of gross errors
    • van der Heijden RTJM, Heijnen JJ, Hellinga C, Romein B Luyben KChAM (1994) Linear constraint relations in biochemical reaction systems: II. Diagnosis and estimation of gross errors. Biotechnol Bioeng 43: 11 20.
    • (1994) Biotechnol Bioeng , vol.43 , pp. 11-20
    • Der Heijden Rtjm, V.1    Heijnen, J.J.2    Hellinga, C.3    Romein, B.4    Kcham, L.5
  • 45
  • 46
    • 0021071243 scopus 로고
    • Structural heterogeneity in populations of the budding yeast Saccharomyces cerevisiae
    • Vanoni M, Vai M, Popolo L Alberghina L (1983) Structural heterogeneity in populations of the budding yeast Saccharomyces cerevisiae. J Bacteriol 156: 1282 1291.
    • (1983) J Bacteriol , vol.156 , pp. 1282-1291
    • Vanoni, M.1    Vai, M.2    Popolo, L.3    Alberghina, L.4
  • 47
    • 0034813964 scopus 로고    scopus 로고
    • Possible pitfalls of flux calculations based on 13C-labeling
    • van Winden WA, Verheijen PJT Heijnen JJ (2001) Possible pitfalls of flux calculations based on 13C-labeling. Metabol Eng 3: 151 162.
    • (2001) Metabol Eng , vol.3 , pp. 151-162
    • Van Winden, W.A.1    Verheijen, P.J.T.2    Heijnen, J.J.3
  • 48
    • 15044347498 scopus 로고    scopus 로고
    • Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of 13C-labeled primary metabolites
    • van Winden WA, van Dam JC, Ras C, Kleijn RJ, Vinke JL, van Gulik WM Heijnen JJ (2005) Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of 13C-labeled primary metabolites. FEMS Yeast Res 5: 559 568.
    • (2005) FEMS Yeast Res , vol.5 , pp. 559-568
    • Van Winden, W.A.1    Van Dam, J.C.2    Ras, C.3    Kleijn, R.J.4    Vinke, J.L.5    Van Gulik, W.M.6    Heijnen, J.J.7
  • 49
    • 0032808002 scopus 로고    scopus 로고
    • Synchronization of yeast cell populations
    • Walker GM (1999) Synchronization of yeast cell populations. Methods Cell Sci 21: 87 93.
    • (1999) Methods Cell Sci , vol.21 , pp. 87-93
    • Walker, G.M.1
  • 50
    • 0020812944 scopus 로고
    • Application of macroscopic balances to the identification of gross measurement errors
    • Wang NS Stephanopoulos G (1983) Application of macroscopic balances to the identification of gross measurement errors. Biotechnol Bioeng 25: 2177 2208.
    • (1983) Biotechnol Bioeng , vol.25 , pp. 2177-2208
    • Wang, N.S.1    Stephanopoulos, G.2
  • 51
    • 17144387903 scopus 로고    scopus 로고
    • From stationary to instationary metabolic flux analysis
    • Wiechert W Nöh K (2005) From stationary to instationary metabolic flux analysis. Adv Biochem Eng/Biotechnol 92: 145 172.
    • (2005) Adv Biochem Eng/Biotechnol , vol.92 , pp. 145-172
    • Wiechert, W.1    Nöh, K.2
  • 52
    • 0035002653 scopus 로고    scopus 로고
    • Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum
    • Wittmann C Heinzle E (2001) Application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum. Eur J Biochem 268: 2441 2455.
    • (2001) Eur J Biochem , vol.268 , pp. 2441-2455
    • Wittmann, C.1    Heinzle, E.2
  • 53
    • 16444380495 scopus 로고    scopus 로고
    • Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae
    • Wittmann C, Hans M, van Winden WA, Ras C Heijnen JJ (2005) Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae. Biotechnol Bioeng 89: 839 847.
    • (2005) Biotechnol Bioeng , vol.89 , pp. 839-847
    • Wittmann, C.1    Hans, M.2    Van Winden, W.A.3    Ras, C.4    Heijnen, J.J.5
  • 55
    • 33646586048 scopus 로고    scopus 로고
    • Short-term metabolome dynamics and carbon, electron, and ATP balances in chemostat-grown Saccharomyces cerevisiae CEN.PK 113-7D following a glucose pulse
    • Wu L, van Dam J, Schipper D, Kresnowati MTAP, Proell AM, Ras C, van Winden WA, van Gulik WM Heijnen JJ (2006b) Short-term metabolome dynamics and carbon, electron, and ATP balances in chemostat-grown Saccharomyces cerevisiae CEN.PK 113-7D following a glucose pulse. Appl Environ Microbiol 72: 3566 3577.
    • (2006) Appl Environ Microbiol , vol.72 , pp. 3566-3577
    • Wu, L.1    Van Dam, J.2    Schipper, D.3    Mtap, K.4    Proell, A.M.5    Ras, C.6    Van Winden, W.A.7    Van Gulik, W.M.8    Heijnen, J.J.9
  • 56
    • 0029239589 scopus 로고
    • Intracellular flux analysis in hybridomas using mass balances and in vitro 13C-NMR
    • Zupke C Stephanopoulos G (1995) Intracellular flux analysis in hybridomas using mass balances and in vitro 13C-NMR. Biotechnol Bioeng 45: 292 303.
    • (1995) Biotechnol Bioeng , vol.45 , pp. 292-303
    • Zupke, C.1    Stephanopoulos, G.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.