Production and characterization of recombinant perdeuterated cholesterol oxidase

Analytical Biochemistry

Volumn 485, Issue , 2015, Pages 102-108

  Golden, Emily ^a   Attwood, Paul V ^a   Duff, Anthony P ^b   Meilleur, Flora ^c,d   Vrielink, Alice ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b AUSTRALIAN NUCLEAR SCIENCE AND TECHNOLOGY ORGANISATION   (Australia)

^c OAK RIDGE NATIONAL LABORATORY   (United States)

^d NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Enzyme kinetics; Flavoenzyme; Perdeuteration; Protein expression; Protein structure; Thermal stability

Indexed keywords

CATALYSIS; CHOLESTEROL; COHERENT SCATTERING; DEUTERIUM; ENZYME KINETICS; HYDROGEN; HYDROGENATION; INCOHERENT SCATTERING; NEUTRON DIFFRACTION; NEUTRON SCATTERING; QUANTUM CHEMISTRY; THERMODYNAMIC STABILITY; X RAY CRYSTALLOGRAPHY;

FLAVIN ADENINE DINUCLEOTIDE; FLAVOENZYMES; NEUTRON CRYSTALLOGRAPHY; PERDEUTERATION; PROTEIN EXPRESSIONS; PROTEIN STRUCTURES; X-RAY CRYSTALLOGRAPHIC STRUCTURES; X-RAY DIFFRACTION STRUCTURE;

ATOMS;

CHOLEST 4 EN 3 ONE; CHOLESTEROL OXIDASE; DEUTERIUM; HYDROGEN; ISOTOPE; OXIDOREDUCTASE; QUERCETIN; RECOMBINANT PERDEUTERATED CHOLESTEROL OXIDASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CULTURE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MECHANISM; FLUOROMETRY; HYDROGENATION; NEUTRON DIFFRACTION; NEUTRON SCATTERING; NONHUMAN; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTON TRANSPORT; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOTOPE LABELING; PROCEDURES;

CHOLESTEROL OXIDASE; CRYSTALLOGRAPHY, X-RAY; DEUTERIUM; ESCHERICHIA COLI; ISOTOPE LABELING; RECOMBINANT PROTEINS;

EID: 84936938563     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.06.008     Document Type: Article

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