Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants

Biochemistry

Volumn 38, Issue 14, 1999, Pages 4277-4286

  Yue, Q Kimberley ^a   Kass, Ignatius J ^b   Sampson, Nicole S ^b   Vrielink, Alice ^a  

^a MCGILL UNIVERSITY   (Canada)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; CHOLEST 4 EN 3 ONE; CHOLESTEROL; CHOLESTEROL OXIDASE; FLAVINE ADENINE NUCLEOTIDE; GLUTAMIC ACID; GLUTAMINE; HISTIDINE; ISOENZYME; MUTANT PROTEIN; WATER;

ALPHA HELIX; AMINO ACID SUBSTITUTION; ARTICLE; BREVIBACTERIUM; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENTROPY; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; HYDROGEN BOND; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STEREOCHEMISTRY; STREPTOMYCES; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

ASPARAGINE; BINDING SITES; CHOLESTEROL OXIDASE; CRYSTALLOGRAPHY, X-RAY; FLAVIN-ADENINE DINUCLEOTIDE; GLUTAMIC ACID; GLUTAMINE; HISTIDINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; STREPTOMYCES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; WATER;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); BREVIBACTERIUM STEROLICUM; STREPTOMYCES; UNCULTURED ACTINOMYCETE;

EID: 0033528745     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi982497j     Document Type: Article

References (33)

1. Vrielink, A., Lloyd, L. F., and Blow, D. M. (1991) J. Mol. Biol. 219, 533-554.
2. Li, J., Vrielink, A., Brick, P., and Blow, D. M. (1993) Biochemistry 32, 11507-11515.
3. Lange, Y. (1992) J. Lipid Res. 33, 315-321.
4. Purcell, J. P., Greenplate, J. T., Jennings, M. G., Tyerse, J. S., Pershing, J. C., Sims, S. R., Prinsen, M. J., Corbin, D. R., Tran, M., Sammons, R. D., and Stonard, R. J. (1993) Biochem. Biophys. Res. Commun. 196, 1406-1413.
5. Greenplate, J. R., Duck, N. B., Pershing, J. C., and Purcell, J. P. (1995) Entomol. Exp. Appl. 74, 253-258.
6. Corbin, D. R., Greenplate, J. T., and Purcell, J. P. (1998) HortScience 33, 614-617.
7. Ghoshroy, K. B., Zhu, W., and Sampson, N. S. (1997) Biochemistry 36, 6133-6140.
8. Uwajima, T., Yagi, H., Nakamurs, S., and Terada, O. (1973) Agric. Biol. Chem. 37, 2345-2350.
9. Tomioka, H., Kagawa, M., and Nakamura, S. (1976) J. Biochem. 79, 903-915.
10. Arima, K., Nagasawa, M., Bae, M., and Tamura, G. (1969) Agric. Biol. Chem. 33, 1636-1643.
11. Kerenyi, F., Szentirmai, A., and Natonek, M. (1975) Acta Microbiol. Acad. Sci. Hung. 22, 487-496.
12. Cheetham, P. S. J., Dunnill, P., and Lilly, M. D. (1982) Biochem. J. 201, 515-521.
13. Gadda, G., Wels, G., Pollegioni, L., Zucchelli, S., Ambrosius, D., Pilone, M. S., and Ghisla, S. (1997) Eur. J. Biochem. 250, 369-376.
14. Ohta, T., Fujishiro, K., Yamaguchi, K., Tamura, Y., Aisaka, K., Uwajima, T., and Hasegawa, M. (1991) Gene 103, 93-96.
15. Kass, I. J., and Sampson, N. S. (1998) Biochemistry (in press).
16. Sampson, N. S., and Kass, I. J. (1997) J. Am. Chem. Soc. 119, 855-862.
17. Sampson, N. S., and Chen, X. (1998) Protein Expression Purif. 12, 347-352.
18. Sampson, N. S., Kass, I. J., and Ghoshroy, K. B. (1998) Biochemistry 37, 5770-5778.
19. Stura, E., and Wilson, I. A. (1990) Methods: A companion to methods in enzymology, Vol. 1, pp 38-49, Academic Press, New York.
20. Otwinowski, Z. (1993) in Data collection and Processing: Proceedings of the CCP4 Study Weekend (Sawyer, L., Isaccs, N., and Bailey, S., Eds.) pp 56-62, SERC Daresbury Laboratory, Warrington, U.K.
21. Navaza, J. (1991) Acta Crystallogr. A50, 157-163.
22. Jones, T. A., Zou, J.-Y., Cowan, S. W., and Kjeldgaard, M. (1991) Acta Crystallogr. A47, 110-119.
23. Brünger, A. T., Kuriyan, J., and Karplus, M. (1987) Science 235, 458-460.
24. Ishizaki, R., Hirayama, N., Shinkawa, H., Nimi, O., and Murooka, Y. (1989) J. Bacteriol. 171, 596-601.
25. Carrière, F., Thirstrup, K., Hjorth, S., Ferrato, F., Nielsen, P. F., Withers-Martinez, C., Cambillau, C., Boel, E., Thim, L., and Verger, R. (1997) Biochemistry 36, 239-248.
26. Egloff, M.-P., Marguet, F., Buono, F., Berger, R., Cambillau, C., and Tilbeurgh, H. V. (1995) Biochemistry 34, 2751-2762.
27. Derewenda, U., Brzozowski, A. M., Lawson, D. M., and Derewenda, Z. S. (1992) Biochemistry 31, 1532-1541.
28. Cambillau, C., Longhi, S., Nicolas, A., and Chrislaine, M. (1996) Curr. Opin. Struct. Biol. 6, 449-455.
29. Kiess, M., Hecht, H.-J., and Kalisz, H. M. (1998) Eur. J. Biochem. 252, 90-99.
30. Cavener, D. (1992) J. Mol. Biol. 223, 811-814.
31. Hecht, H. J., Kalisz, H. M., Hendle, J., Schmid, R. D., and Schomburg, D. (1993) J. Mol. Biol. 229, 153-172.
32. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.
33. Merritt, E. A., and Bacon, D. J. (1997) Methods Enzymol. 277, 505-525.