High yield expression and efficient purification of deuterated human protein galectin-2

Food and Bioproducts Processing

Volumn 90, Issue 3, 2012, Pages 563-572

  Chen, Xiaojing ^a   Wilde, Karyn L ^b   Wang, Hui ^a   Lake, Vanessa ^b   Holden, Peter J ^b   Middelberg, Anton P J ^a   He, Lizhong ^a,c   Duff, Anthony P ^b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b AUSTRALIAN NUCLEAR SCIENCE AND TECHNOLOGY ORGANISATION   (Australia)

^c MONASH UNIVERSITY   (Australia)

Author keywords

Affinity chromatography; Deuteration; Human galectin 2; Ion exchange chromatography; Protein aggregates

Indexed keywords

ANTIBODY RECOGNITION; BIO-SEPARATION; BIOLOGICAL MACROMOLECULE; BIOPROCESSING; CONTRAST VARIATION; DEUTERATED PROTEINS; DEUTERATIONS; DOT BLOTS; HIGH DENSITY; HIGH PURITY; HIGH YIELD; HUMAN GALECTIN-2; HUMAN PROTEINS; HYDROGEN ATOMS; ION-EXCHANGE CHROMATOGRAPHY; KANAMYCINS; N-TERMINAL SEQUENCING; OXYGEN LIMITATION; PROTEIN AGGREGATES; SOLE CARBON SOURCE; STRUCTURAL STUDIES; THERAPEUTIC APPLICATION; WET WEIGHT;

AFFINITY CHROMATOGRAPHY; AGGREGATES; ESCHERICHIA COLI; FERMENTATION; GLYCEROL; MASS SPECTROMETRY; PROTEINS; PURIFICATION; SUGARS;

DEUTERIUM;

ANIMALIA; ESCHERICHIA COLI;

EID: 84862829256     PISSN: 09603085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fbp.2011.12.004     Document Type: Article

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