Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA

BMC Research Notes

Volumn 8, Issue 1, 2015, Pages

  Johnson, Lisa A ^a   Chaban, Bonnie ^a,b   Harding, John C S ^a   Hill, Janet E ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

cpn60; Gradient PCR; Host microbiome; Microbiome profiling; PCR conditions; Pig microbiome; Pyrosequencing

Indexed keywords

BACTERIA (MICROORGANISMS); SUIDAE;

BACTERIAL PROTEIN; CHAPERONIN 60;

ANIMAL; DNA SEQUENCE; GENETICS; MICROBIOLOGY; MICROFLORA; PIG; POLYMERASE CHAIN REACTION; PROCEDURES;

ANIMALS; BACTERIAL PROTEINS; CHAPERONIN 60; MICROBIOTA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SWINE;

EID: 84934972774     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/s13104-015-1170-4     Document Type: Article

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