Improvement of the Representation of Bifidobacteria in Fecal Microbiota Metagenomic Libraries by Application of the cpn60 Universal Primer Cocktail

Applied and Environmental Microbiology

Volumn 76, Issue 13, 2010, Pages 4550-4552

  Hill, Janet E ^a   Ursla Fernando, W M ^a,e   Zello, Gordon A ^b   Tyler, Robert T ^a   Dahl, Wendy J ^b,c   Van Kessel, Andrew G ^a,d  

^a Department of Veterinary Microbiology ^*  (United States)

^b Division of Nutrition and Dietetics   (United States)

^c UNIVERSITY OF FLORIDA   (United States)

^d UNIVERSITY OF SASKATCHEWAN   (Canada)

^e CANADIAN GRAIN COMMISSION   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA; ACTINOBACTERIA; BIFIDOBACTERIA; CLONE LIBRARY; FECAL MICROBIOTA; HUMAN FECAL; METAGENOMIC LIBRARIES; MICROBIAL COMMUNITIES; PCR PRODUCTS;

CLONING; RNA;

LIBRARIES;

BACTERIAL DNA; CHAPERONIN; PRIMER DNA; RNA 16S;

BACTERIUM; FECES; GENE EXPRESSION; GENOMICS; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; TAXONOMY;

ADOLESCENT; ADULT; AGED; ARTICLE; BIFIDOBACTERIUM; CLASSIFICATION; COMPARATIVE STUDY; DNA SEQUENCE; EVALUATION; FECES; GENE LIBRARY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METAGENOMICS; MICROBIOLOGY; MIDDLE AGED; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ADOLESCENT; ADULT; AGED; BIFIDOBACTERIUM; CHAPERONIN 60; DNA PRIMERS; DNA, BACTERIAL; FECES; GENE LIBRARY; HUMANS; METAGENOMICS; MIDDLE AGED; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; YOUNG ADULT;

ACTINOBACTERIA;

EID: 77954254522     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01510-09     Document Type: Article

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