Three-dimensional imaging of adherent cells using FIB/SEM and STEM

Methods in Molecular Biology

Volumn 1117, Issue , 2014, Pages 617-638

  Villinger, Clarissa ^a,b   Schauflinger, Martin ^a,b   Gregorius, Heiko ^a   Kranz, Christine ^a   Höhn, Katharina ^c   Nafeey, Soufi ^a   Walther, Paul ^a  

^a UNIVERSITY OF ULM   (Germany)

^b UNIVERSITY HOSPITAL ULM   (Germany)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

FIB SEM tomography; Freeze substitution; High pressure freezing; Sapphire discs; Scanning transmission electron microscopy; Serial sectioning

Indexed keywords

RESIN;

ADHERENT CELL; ARTICLE; ELECTRON BEAM; ELECTRON TOMOGRAPHY; EUKARYOTIC CELL; FOCUSED ION BEAM TOMOGRAPHY; PRIORITY JOURNAL; SCANNING TRANSMISSION ELECTRON MICROSCOPY; THREE DIMENSIONAL IMAGING; TOMOGRAPHY; TRANSMISSION ELECTRON MICROSCOPY;

CELL CULTURE TECHNIQUES; CELLS, CULTURED; ELECTRON MICROSCOPE TOMOGRAPHY; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, ELECTRON, SCANNING TRANSMISSION; SOFTWARE;

EID: 84934438678     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-776-1_27     Document Type: Article

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