Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides

BMC Biochemistry

Volumn 16, Issue 1, 2015, Pages

  Poulsen, Jesper Buchhave ^a   Kjær, Karina Hansen ^a   Justesen, Just ^a   Martensen, Pia Moller ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

2 5 oligoadenylate nuclease; 2 5 oligoadenylate synthetase (OAS); 5 dephosphorylated forms of 2 5As (2 5A core molecules); 5 triphosphorylated, 2 5 linked oligoadenylate polyribonucleotides (2 5As); Enzyme assays

Indexed keywords

2',5' LINKED OLIGOADENYLATE POLYRIBONUCLEOTIDE; 2',5' OLIGOADENYLATE SYNTHETASE; DEOXYADENOSINE TRIPHOSPHATE; NUCLEASE; PHOSPHODIESTERASE; PHOSPHODIESTERASE 12; POLYRIBONUCLEOTIDE; RECOMBINANT PROTEIN; SYNTHETASE; TETRAMER; UNCLASSIFIED DRUG; 2',5'-OLIGOADENYLATE; ADENINE NUCLEOTIDE; EXORIBONUCLEASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; OAS1 PROTEIN, HUMAN; OLIGORIBONUCLEOTIDE; PDE12 PROTEIN, HUMAN;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DEGRADATION KINETICS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; IN VITRO STUDY; ION EXCHANGE CHROMATOGRAPHY; PROTEIN EXPRESSION; BIOSYNTHESIS; HELA CELL LINE; HUMAN; METABOLISM;

2',5'-OLIGOADENYLATE SYNTHETASE; ADENINE NUCLEOTIDES; ENZYME ASSAYS; EXORIBONUCLEASES; HELA CELLS; HUMANS; NAD; OLIGORIBONUCLEOTIDES; POLYRIBONUCLEOTIDES; SUBSTRATE SPECIFICITY;

EID: 84932150526     PISSN: None     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/s12858-015-0043-8     Document Type: Article

References (52)

1. Samuel CE. Antiviral actions of interferon. Interferon-regulated cellular proteins and their surprisingly selective antiviral activities. Virology. 1991;183(1):1-11.
2. Samuel CE. Antiviral actions of interferons. Clin Microbiol Rev. 2001;14(4):778-809.
3. Stark GR, Kerr IM, Williams BR, Silverman RH, Schreiber RD. How cells respond to interferons. Annu Rev Biochem. 1998;67:227-64.
4. Takaoka A, Yanai H. Interferon signalling network in innate defence. Cell Microbiol. 2006;8(6):907-22.
5. Hovanessian AG, Justesen J. The human 2′-5′oligoadenylate synthetase family: unique interferon-inducible enzymes catalyzing 2′-5′ instead of 3′-5′ phosphodiester bond formation. Biochimie. 2007;89(6-7):779-88.
6. Justesen J, Hartmann R, Kjeldgaard NO. Gene structure and function of the 2′-5′-oligoadenylate synthetase family. Cell Mol Life Sci. 2000;57(11):1593-612.
7. Eskildsen S, Hartmann R, Kjeldgaard NO, Justesen J. Gene structure of the murine 2′-5′-oligoadenylate synthetase family. Cell Mol Life Sci. 2002;59(7):1212-22.
8. Kumar S, Mitnik C, Valente G, Floyd-Smith G. Expansion and molecular evolution of the interferon-induced 2′-5′ oligoadenylate synthetase gene family. Mol Biol Evol. 2000;17(5):738-50.
9. Carroll SS, Chen E, Viscount T, Geib J, Sardana MK, Gehman J, et al. Cleavage of oligoribonucleotides by the 2′,5′-oligoadenylate- dependent ribonuclease L. J Biol Chem. 1996;271(9):4988-92.
10. Floyd-Smith G, Slattery E, Lengyel P. Interferon action: RNA cleavage pattern of a (2′-5′)oligoadenylate-dependent endonuclease. Science. 1981;212(4498):1030-2.
11. Wreschner DH, McCauley JW, Skehel JJ, Kerr IM. Interferon action-sequence specificity of the ppp(A2′p)nA-dependent ribonuclease. Nature. 1981;289(5796):414-7.
12. Silverman RH, Cayley PJ, Knight M, Gilbert CS, Kerr IM. Control of the ppp(a2′p)nA system in HeLa cells. Effects of interferon and virus infection. Eur J Biochem. 1982;124(1):131-8.
13. Hartmann R, Olsen HS, Widder S, Jorgensen R, Justesen J. p59OASL, a 2′-5′ oligoadenylate synthetase like protein: a novel human gene related to the 2′-5′ oligoadenylate synthetase family. Nucleic Acids Res. 1998;26(18):4121-8.
14. Justesen J, Ferbus D, Thang MN. 2′5′ oligoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ oligoadenylates and 2′5′ co-oligonucleotides. Nucleic Acids Res. 1980;8(14):3073-85.
15. Ferbus D, Justesen J, Besancon F, Thang MN. The 2′5′ oligoadenylate synthetase has a multifunctional 2′5′ nucleotidyl-transferase activity. Biochem Biophys Res Commun. 1981;100(2):847-56.
16. Castelli JC, Hassel BA, Wood KA, Li XL, Amemiya K, Dalakas MC, et al. A study of the interferon antiviral mechanism: apoptosis activation by the 2-5A system. J Exp Med. 1997;186(6):967-72.
17. Bisbal C, Martinand C, Silhol M, Lebleu B, Salehzada T. Cloning and characterization of a RNAse L inhibitor. A new component of the interferon-regulated 2-5A pathway. J Biol Chem. 1995;270(22):13308-17.
18. Kubota K, Nakahara K, Ohtsuka T, Yoshida S, Kawaguchi J, Fujita Y, et al. Identification of 2′-phosphodiesterase, which plays a role in the 2-5A system regulated by interferon. J Biol Chem. 2004;279(36):37832-41.
19. Poulsen JB, Andersen KR, Kjaer KH, Vestergaard AL, Justesen J, Martensen PM. Characterization of human phosphodiesterase 12 and identification of a novel 2′-5′ oligoadenylate nuclease - The ectonucleotide pyrophosphatase/phosphodiesterase 1. Biochimie. 2012;94(5):1098-107.
20. Gusho E, Zhang R, Jha BK, Thornbrough JM, Dong B, Gaughan C, et al. Murine AKAP7 has a 2′,5′-phosphodiesterase domain that can complement an inactive murine coronavirus ns2 gene. mBio. 2014;5(4):e01312-4.
21. Zhao L, Jha BK, Wu A, Elliott R, Ziebuhr J, Gorbalenya AE, et al. Antagonism of the interferon-induced OAS-RNase L pathway by murine coronavirus ns2 protein is required for virus replication and liver pathology. Cell Host Microbe. 2012;11(6):607-16.
22. Zhang R, Jha BK, Ogden KM, Dong B, Zhao L, Elliott R, et al. Homologous 2′,5′-phosphodiesterases from disparate RNA viruses antagonize antiviral innate immunity. Proc Natl Acad Sci U S A. 2013;110(32):13114-9.
23. Poulsen JB, Andersen KR, Kjaer KH, Durand F, Faou P, Vestergaard AL, et al. Human 2′-phosphodiesterase localizes to the mitochondrial matrix with a putative function in mitochondrial RNA turnover. Nucleic Acids Res. 2011;39(9):3754-70.
24. van Driel IR, Wilks AF, Pietersz GA, Goding JW. Murine plasma cell membrane antigen PC-1: molecular cloning of cDNA and analysis of expression. Proc Natl Acad Sci U S A. 1985;82(24):8619-23.
25. Brown RL, August SL, Williams CJ, Moss SB. AKAP7gamma is a nuclear RI-binding AKAP. Biochem Biophys Res Commun. 2003;306(2):394-401.
26. Bayard BA, Gabrion JB. 2′,5′-Oligoadenylate-dependent RNAse located in nuclei: biochemical characterization and subcellular distribution of the nuclease in human and murine cells. Biochem J. 1993;296(Pt 1):155-60.
27. Besse S, Rebouillat D, Marie I, Puvion-Dutilleul F, Hovanessian AG. Ultrastructural localization of interferon-inducible double-stranded RNA-activated enzymes in human cells. Exp Cell Res. 1998;239(2):379-92.
28. Chebath J, Benech P, Hovanessian A, Galabru J, Revel M. Four different forms of interferon-induced 2′,5′-oligo(A) synthetase identified by immunoblotting in human cells. J Biol Chem. 1987;262(8):3852-7.
29. Hovanessian AG, Laurent AG, Chebath J, Galabru J, Robert N, Svab J. Identification of 69-kd and 100-kd forms of 2-5A synthetase in interferon-treated human cells by specific monoclonal antibodies. EMBO J. 1987;6(5):1273-80.
30. Hersh CL, Brown RE, Roberts WK, Swyryd EA, Kerr IM, Stark GR. Simian virus 40-infected, interferon-treated cells contain 2′,5′-oligoadenylates which do not activate cleavage of RNA. J Biol Chem. 1984;259(3):1731-7.
31. Rice AP, Roberts WK, Kerr IM. 2-5A accumulates to high levels in interferon-treated, vaccinia virus-infected cells in the absence of any inhibition of virus replication. J Virol. 1984;50(1):220-8.
32. Imai J, Torrence PF. An efficient chemical synthesis of adenylyl(2′ goes to 5′)adenylyl(2′ goes to 5′)adenosine [(2′-5′)-oligo(A)]. Methods Enzymol. 1981;79(Pt B):233-44.
33. Kitade Y, Tanida Y, Tuboi T, Yoshimura A, Nakanishi M, Yatome C. Synthesis of 2-5As possessing base-modified adenosines and their activities to human recombinant RNase L. Nucleic Acids Symp Ser. 2000;44:29-30.
34. Morin B, Rabah N, Boretto-Soler J, Tolou H, Alvarez K, Canard B. High yield synthesis, purification and characterisation of the RNase L activators 5′-triphosphate 2′-5′-oligoadenylates. Antiviral Res. 2010;87(3):345-52.
35. Rusch L, Dong B, Silverman RH. Monitoring activation of ribonuclease L by 2′,5′-oligoadenylates using purified recombinant enzyme and intact malignant glioma cells. Methods Enzymol. 2001;342:10-20.
36. Thakur CS, Jha BK, Dong B, Das Gupta J, Silverman KM, Mao H, et al. Small-molecule activators of RNase L with broad-spectrum antiviral activity. Proc Natl Acad Sci U S A. 2007;104(23):9585-90.
37. Ball LA. Induction, purification, and properties of 2′5′ oligoadenylate synthetase. Ann N Y Acad Sci. 1980;350:486-96.
38. Kjaer KH, Poulsen JB, Reintamm T, Saby E, Martensen PM, Kelve M, et al. Evolution of the 2′-5′-oligoadenylate synthetase family in eukaryotes and bacteria. J Mol Evol. 2009;69(6):612-24.
39. Zilberstein A, Kimchi A, Schmidt A, Revel M. Isolation of two interferon-induced translational inhibitors: a protein kinase and an oligo-isoadenylate synthetase. Proc Natl Acad Sci U S A. 1978;75(10):4734-8.
40. Hearl WG, Johnston MI. Accumulation of 2′,5′-oligoadenylates in encephalomyocarditis virus-infected mice. J Virol. 1987;61(5):1586-92.
41. Schmidt A, Chernajovsky Y, Shulman L, Federman P, Berissi H, Revel M. An interferon-induced phosphodiesterase degrading (2′-5′) oligoisoadenylate and the C-C-A terminus of tRNA. Proc Natl Acad Sci U S A. 1979;76(10):4788-92.
42. Severin ES, Itkes AV, Kartasheva ON, Tunitskaya VL, Turpaev KT, Kafiani CA. Regulation of 2-5 A phosphodiesterase activity by cAMP-dependent phosphorylation: mechanism and biological role. Adv Enzyme Regul. 1985;23:365-76.
43. Saarma M, Toots U, Raukas E, Zhelkovsky A, Pivazian A, Neuman T. Nerve growth factor induces changes in (2′-5′)oligo(A) synthetase and 2′-phosphodiesterase activities during differentiation of PC12 pheochromocytoma cells. Exp Cell Res. 1986;166(1):229-36.
44. Dong B, Xu L, Zhou A, Hassel BA, Lee X, Torrence PF, et al. Intrinsic molecular activities of the interferon-induced 2-5A-dependent RNase. J Biol Chem. 1994;269(19):14153-8.
45. Saby E, Poulsen JB, Justesen J, Kelve M, Uriz MJ. 2′-phosphodiesterase and 2′,5′-oligoadenylate synthetase activities in the lowest metazoans, sponge [porifera]. Biochimie. 2009;91:1531-4.
46. Donovan J, Whitney G, Rath S, Korennykh A. Structural mechanism of sensing long dsRNA via a noncatalytic domain in human oligoadenylate synthetase 3. Proc Natl Acad Sci U S A. 2015;112(13):3949-54.
47. Ibsen MS, Gad HH, Thavachelvam K, Boesen T, Despres P, Hartmann R. The 2′-5′-oligoadenylate synthetase 3 enzyme potently synthesizes the 2′-5′-oligoadenylates required for RNase L activation. J Virol. 2014;88(24):14222-31.
48. Eskildsen S, Justesen J, Schierup MH, Hartmann R. Characterization of the 2′-5′-oligoadenylate synthetase ubiquitin-like family. Nucleic Acids Res. 2003;31(12):3166-73.
49. Cayley PJ, Kerr IM. Synthesis, characterisation and biological significance of (2′-5′)oligoadenylate derivatives of NAD+, ADP-ribose and adenosine(5′)tetraphospho(5′)adenosine. Eur J Biochem. 1982;122(3):601-8.
50. Johnston MI, Hearl WG. Purification and characterization of a 2′-phosphodiesterase from bovine spleen. J Biol Chem. 1987;262(17):8377-82.
51. Silverman RH, Weiss SR. Viral phosphodiesterases that antagonize double-stranded RNA signaling to RNase L by degrading 2-5A. J Interferon Cytokine Res. 2014;34(6):455-63.
52. Päri M, Kuusksalu A, Lopp A, Kjaer KH, Justesen J, Kelve M. Enzymatically active 2′, 5′-oligoadenylate synthetases are widely distributed among Metazoa, including protostome lineage. Biochimie. 2014;97:200-9.