Mortalin staining pattern as a reporter for cell based anti-cancer drug screening

Mortalin Biology: Life, Stress and Death

Volumn 9789400730274, Issue , 2012, Pages 307-322

  Gao, Ran ^a   Kaul, Zeenia ^b   Yaguchi, Tomoko ^a   Wadhwa, Renu ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b NATIONWIDE CHILDREN S HOSPITAL   (United States)

Author keywords

Drug screening; Immunostaining; Mortalin; Pancytoplasmic; Perinuclear

Indexed keywords

EID: 84931439004     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-94-007-3027-4_20     Document Type: Chapter

References (60)

1. Bertram MJ, Berube NG, Hang-Swanson X, Ran Q, Leung JK, Bryce S, Spurgers K, Bick RJ, Baldini A, NingY et al (1999) Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes. Mol Cell Biol 19:1479-1485
2. Casillas MA, Jr Lopatina N, Andrews LG, Tollefsbol TO (2003) Transcriptional control of the DNA methyltransferases is altered in aging and neoplastically-transformed human fibroblasts. Mol Cell Biochem 252:33-43
3. Chiba Y, Kubota T, Watanabe M, Matsuzaki SW, Otani Y, Teramoto T, Matsumoto Y, Koya K, Kitajima M (1998a) MKT-077, localized lipophilic cation: antitumor activity against human tumor xenografts serially transplanted into nude mice. Anticancer Res 18:1047-1052
4. ChibaY, Kubota T,Watanabe M, OtaniY, Teramoto T, MatsumotoY, Koya K, Kitajima M (1998b) Selective antitumor activity of MKT-077, a delocalized lipophilic cation, on normal cells and cancer cells in vitro. J Surg Oncol 69:105-110
5. Das PM, Singal R (2004) DNA methylation and cancer. J Clin Oncol 22:4632-4642
6. Deocaris CC, Widodo N, Shrestha BG, Kaur K, Ohtaka M, Yamasaki K, Kaul SC, Wadhwa R (2007) Mortalin sensitizes human cancer cells to MKT-077-induced senescence. Cancer Lett 252:259-269
7. Dundas SR, Lawrie LC, Rooney PH, Murray GI (2005) Mortalin is over-expressed by colorectal adenocarcinomas and correlates with poor survival. J Pathol 205:74-81
8. Eads CA, Danenberg KD, Kawakami K, Saltz LB, Danenberg PV, Laird PW (1999) CpG island hypermethylation in human colorectal tumors is not associated with DNA methyltransferase overexpression. Cancer Res 59:2302-2306
9. Enokida H, Shiina H, Igawa M, OgishimaT, KawakamiT, BassettWW,AnastJW, Li LC, Urakami S, TerashimaMet al (2004) CpG hypermethylation of MDR1gene contributes to the pathogenesis and progression of human prostate cancer. Cancer Res 64:5956-5962
10. Fairweather DS, Fox M, Margison GP (1987) The in vitro lifespan of MRC-5 cells is shortened by 5-azacytidine-induced demethylation. Exp Cell Res 168:153-159
11. Gao R, Yaguchi T, Horimoto K, Ishii T, Kaul SC, Wadhwa R (2010) Identification of anti-cancer shRNAs based on the staining pattern of mortalin. Tiss Cult Res Communn 29:147-153
12. Hara E, Smith R, Parry D, Tahara H, Stone S, Peters G (1996) Regulation of p16CDKN2 expression and its implications for cell immortalization and senescence. Mol Cell Biol 16:859-867
13. Hensler PJ, Annab LA, Barrett JC, Pereira-Smith OM (1994) A gene involved in control of human cellular senescence on human chromosome 1q. Mol Cell Biol 14:2291-2297
14. Hiltunen MO, Turunen MP, Hakkinen TP, Rutanen J, Hedman M, Makinen K, Turunen AM, Aalto-Setala K, Yla-Herttuala S (2002) DNA hypomethylation and methyltransferase expression in atherosclerotic lesions. Vasc Med 7:5-11
15. Holliday R (1987) The inheritance of epigenetic defects. Science 238:163-170
16. Huang Y, de la Chapelle A, Pellegata NS (2003) Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma. Int J Cancer 104:735-744
17. Juttermann R, Li E, Jaenisch R (1994) Toxicity of 5-aza-2-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyl transferase rather thanDNAdemethylation. Proc Natl Acad Sci U S A 91:11797-11801
18. Kaneda A, Kaminishi M,Yanagihara K, Sugimura T, Ushijima T (2002) Identification of silencing of nine genes in human gastric cancers. Cancer Res 62:6645-6650
19. Kaul SC, Taira K, Pereira-Smith OM, Wadhwa R (2002) Mortalin: present and prospective. Exp Gerontol 37:1157-1164
20. Kaul Z,Yaguchi T, Chiura HX, Kaul SC,Wadhwa R (2007) Quantum dot-based mortalin staining as a visual assay for detection of induced senescence in cancer cells. Ann N Y Acad Sci 1100:368-372
21. Kulaeva OI, Draghici S, Tang L, Kraniak JM, Land SJ, Tainsky MA (2003) Epigenetic silencing of multiple interferon pathway genes after cellular immortalization. Oncogene 22:4118-4127
22. LichtensteinAV, Kisseljova NP (2001) DNA methylation and carcinogenesis. Biochemistry (Mosc) 66:235-255
23. Lin CH, Hsieh SY, Sheen IS, Lee WC, Chen TC, Shyu WC, Liaw YF (2001) Genome-wide hypomethylation in hepatocellular carcinogenesis. Cancer Res 61:4238-4243
24. Maruta H, Tikoo A, Shakri R, Shishido T (1999) The anti-RAS cancer drug MKT-077 is an F-actin cross-linker. Ann NY Acad Sci 886:283-284
25. Michishita E, Nakabayashi K, Suzuki T, Kaul SC, Ogino H, Fujii M, MitsuiY, Ayusawa D (1999) 5-Bromodeoxyuridine induces senescence-like phenomena in mammalian cells regardless of cell type or species. J Biochem 126:1052-1059
26. Mihara M, Erster S, Zaika A, Petrenko O, Chittenden T, Pancoska P, Moll UM (2003) p53 has a direct apoptogenic role at the mitochondria. Mol Cell 11:577-590
27. Mizukoshi E, Suzuki M, Loupatov A, Uruno T, Hayashi H, Misono T, Kaul SC, Wadhwa R, Imamura T (1999) Fibroblast growth factor-1 interacts with the glucose-regulated protein GRP75/mortalin. Biochem J 343:461-466
28. Mizukoshi E, Suzuki M, Misono T, Loupatov A, Munekata E, Kaul SC, Wadhwa R, Imamura T (2001) Cell-cycle dependent tyrosine phosphorylation on mortalin regulates its interaction with fibroblast growth factor-1. Biochem Biophys Res Commun 280:1203-1209
29. Modica-Napolitano JS, Koya K,Weisberg E, Brunelli BT, LiY, Chen LB (1996) Selective damage to carcinoma mitochondria by the rhodacyanine MKT-077. Cancer Res 56:544-550
30. Nakabayashi K, Ogata T, Fujii M, Tahara H, Ide T,Wadhwa R, Kaul SC, MitsuiY,AyusawaD(1997) Decrease in amplified telomeric sequences and induction of senescence markers by introduction of human chromosome 7 or its segments in SUSM-1. Exp Cell Res 235:345-353
31. NingY,Weber JL, KillaryAM, Ledbetter DH, Smith JR, Pereira-SmithOM(1991) Genetic analysis of indefinite division in human cells: evidence for a cell senescence-related gene(s) on human chromosome 4. Proc Natl Acad Sci U S A 88:5635-5639
32. Ogata T, Oshimura M, Namba M, Fujii M, Oishi M, Ayusawa D (1995) Genetic complementation of the immortal phenotype in group D cell lines by introduction of chromosome 7. Jpn J Cancer Res 86:35-40
33. Paddison PJ, CaudyAA, Bernstein E, Hannon GJ, ConklinDS(2002) Short hairpinRNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev 16:948-958
34. Pereira-Smith OM, Smith JR (1983) Evidence for the recessive nature of cellular immortality. Science 221:964-966
35. Petit T, Izbicka E, Lawrence RA, Nalin C,Weitman SD,Von Hoff DD (1999) Activity of MKT 077, a rhodacyanine dye, against human tumor colony- forming units. Anticancer Drugs 10:309-315
36. Sakajiri S, Kumagai T, Kawamata N, Saitoh T, Said JW, Koeffler HP (2005) Histone deacetylase inhibitors profoundly decrease proliferation of human lymphoid cancer cell lines. Exp Hematol 33:53-61
37. Serrano M, LinAW, McCurrach ME, Beach D, LoweSW(1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell 88:593-602
38. Shen H,Wang L, Spitz MR, Hong WK, Mao L,Wei Q (2002)A novel polymorphism in human cytosine DNA-methyltransferase-3B promoter is associated with an increased risk of lung cancer. Cancer Res 62:4992-4995
39. Smith JR, Pereira-Smith OM (1996) Replicative senescence: implications for in vivo aging and tumor suppression. Science 273:63-67
40. Srokowski T, Pfeifer JD, Li J, Olson LM, Rader JS (2004) Expression and localization of GRP75 in human epithelial tumors and normal tissues. Appl Immunohistochem Mol Morphol 12:132-138
41. Sugimoto S, Maass N, TakimotoY, Sato K, Minei S, Zhang M, HoshikawaY, Junemann KP, Jonat W, Nagasaki K (2004) Expression and regulation of tumor suppressor gene maspin in human bladder cancer. Cancer Lett 203:209-215
42. Swafford DS, Middleton SK, Palmisano WA, Nikula KJ, Tesfaigzi J, Baylin SB, Herman JG, Belinsky SA (1997) Frequent aberrant methylation of p16INK4a in primary rat lung tumors. Mol Cell Biol 17:1366-1374
43. Takano S,Wadhwa R, MitsuiY, Kaul SC (2001) Identification and characterization of molecular interactions between glucose-regulated proteins (GRPs) mortalin/GRP75/peptide-binding protein 74 (PBP74) and GRP94. Biochem J 357:393-398
44. Tatsuta N, Suzuki N, Mochizuki T, Koya K, Kawakami M, Shishido T, Motoji N, Kuroiwa H, Shigematsu A, Chen LB (1999) Pharmacokinetic analysis and antitumor efficacy of MKT-077, a novel antitumor agent. Cancer Chemother Pharmacol 43:295-301
45. Tikoo A, Cutler H, Lo SH, Chen LB, Maruta H (1999) Treatment of Ras-induced cancers by the Factin cappers tensin and chaetoglobosin K, in combination with the caspase-1 inhibitor N1445. Cancer J Sci Am 5:293-300
46. Uhrbom L, Nister M, Westermark B (1997) Induction of senescence in human malignant glioma cells by p16INK4A. Oncogene 15:505-514
47. Vertino PM, Issa JP, Pereira-Smith OM, Baylin SB (1994) Stabilization of DNA methyltransferase levels andCpGisland hypermethylation precede SV40-induced immortalization of human fibroblasts. Cell Growth Differ 5:1395-1402
48. Wadhwa R, Kaul SC, Ikawa Y, Sugimoto Y (1993a) Identification of a novel member of mouse hsp70 family: its association with cellular mortal phenotype. J Biol Chem 268:6615-6621
49. Wadhwa R, Kaul SC, MitsuiY, SugimotoY (1993b) Differential subcellular distribution of mortalin in mortal and immortal mouse and human fibroblasts. Exp Cell Res 207:442-448
50. Wadhwa R, Pereira-Smith OM, Reddel RR, Sugimoto Y, Mitsui Y, Kaul SC (1995) Correlation between complementation group for immortality and the cellular distribution of mortalin. Exp Cell Res 216:101-106
51. Wadhwa R, Takano S, Robert M, Yoshida A, Nomura H, Reddel RR, Mitsui Y, Kaul SC (1998) Inactivation of tumor suppressor p53 by mot-2, a hsp70 family member. J Biol Chem 273:29586-29591
52. Wadhwa R, Sugihara T,Yoshida A, Nomura H, Reddel RR, Simpson R, Maruta H, Kaul SC (2000) Selective toxicity of MKT-077 to cancer cells is mediated by its binding to hsp70 family protein, mot-2, and reactivation of p53 function. Cancer Res 60:6818-6821
53. Wadhwa R,Taira K, Kaul SC (2002a)An hsp70 family chaperone, mortalin/mthsp70/PBP74/Grp75: what, when and where? Cell Stress & Chaperones 7:309-316
54. Wadhwa R,Yaguchi T, Hasan MK, MitsuiY, Reddel RR, Kaul SC (2002b) Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein. Exp Cell Res 274:246-253
55. Wadhwa R,Yaguchi T, Hasan MK, Taira K, Kaul SC (2003) Mortalin-MPD (mevalonate pyrophosphate decarboxylase) interactions and their role in control of cellular proliferation. Biochem Biophys Res Commun 302:735-742
56. Wadhwa R, Takano S, Kaur K, Deocaris CC, Pereira-Smith OM, Reddel RR, Kaul SC (2006) Upregulation of mortalin/mthsp70/Grp75 contributes to human carcinogenesis. Int J Cancer 118:2973-2980
57. Walton SP, Wu M, Gredell JA, Chan C (2010) Designing highly active siRNAs for therapeutic applications. FEBS J 277:4806-4813
58. Widodo N, Deocaris CC, Kaur K, Hasan K, Yaguchi T, Yamasaki K, Sugihara T, Ishii T, Wadhwa R, Kaul SC (2007a) Stress chaperones, mortalin, and pex19p mediate 5-aza-2 deoxycytidineinduced senescence of cancer cells by DNA methylation-independent pathway. J Gerontol A Biol Sci Med Sci 62:246-255
59. Widodo N, Kaur K, Shrestha BG, TakagiY, Ishii T,Wadhwa R, Kaul SC (2007b) Selective killing of cancer cells by leaf extract of Ashwagandha: Identification of a tumor inhibitory factor and the first molecular insights to its effect. Clinical Cancer Res 13:2298-2306
60. Zhong ZH, Jiang WQ, Cesare AJ, Neumann AA, Wadhwa R, Reddel RR (2007) Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem 282:29314-29322