Multistrip western blotting: A tool for comparative quantitative analysis of multiple proteins

Methods in Molecular Biology

Volumn 1312, Issue , 2015, Pages 197-226

  Aksamitiene, Edita ^a   Hoek, Jan B ^a   Kiyatkin, Anatoly ^a  

^a NONE

Author keywords

Blotting errors; Cell signaling; Electrophoretic transfer; Gel cutting; High throughput; Quantitative protein analysis; Systems biology; Western blotting

Indexed keywords

MULTIPROTEIN COMPLEX;

ANALYTICAL ERROR; ARTICLE; CELL PERMEABILIZATION; CHEMOLUMINESCENCE; COMPARATIVE STUDY; CYTOLYSIS; IMMUNOBLOTTING; IMMUNODETECTION; IMMUNOPRECIPITATION; INTRACELLULAR SIGNALING; MEASUREMENT ACCURACY; MULTISTRIP WESTERN BLOTTING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN TRANSPORT; QUANTITATIVE ANALYSIS; SYSTEMS BIOLOGY; WESTERN BLOTTING; WHOLE CELL;

EID: 84930966070     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2694-7_23     Document Type: Article

References (37)

1. Soundy P, Harvey B (2005) Western blotting as a diagnostic method. In: Walker JM, Rapley R (eds) Medical biomethods handbook. Humana, Totowa, NJ, pp 43–62
2. Omenn GS (2006) Strategies for plasma proteomic profi ling of cancers. Proteomics 6: 5662–5673
3. Hueber W, Robinson WH (2006) Proteomic biomarkers for autoimmune disease. Proteomics 6:4100–4105
4. Fardilha M, Wu W, Sa R, Fidalgo S, Sousa C, Mota C, da Cruz Silva OA, da Cruz Silva EF (2004) Alternatively spliced protein variants as potential therapeutic targets for male infertility and contraception. Ann N Y Acad Sci 1030:468–478
5. Ducruet AP, Vogt A, Wipf P, Lazo JS (2005) Dual specifi city protein phosphatases: therapeutic targets for cancer and Alzheimer’s disease. Annu Rev Pharmacol Toxicol 45: 725–750
6. Gaiger A, Heintel D, Jager U (2004) Novel molecular diagnostic and therapeutic targets in chronic lymphocytic leukaemia. Eur J Clin Invest 34(Suppl 2):25–30
7. Sawyer TK (2004) Cancer metastasis therapeutic targets and drug discovery: emerging smallmolecule protein kinase inhibitors. Expert Opin Investig Drugs 13:1–19
8. Krause DS, Van Etten RA (2005) Tyrosine kinases as targets for cancer therapy. N Engl J Med 353:172–187
9. Kholodenko BN (2006) Cell-signalling dynamics in time and space. Nat Rev Mol Cell Biol 7:165–176
10. Kurien BT, Scofi eld RH (2006) Western blotting. Methods 38:283–293
11. Schilling M, Maiwald T, Bohl S, Kollmann M, Kreutz C, Timmer J, Klingmuller U (2005) Quantitative data generation for systems biology: the impact of randomisation, calibrators and normalisers. Syst Biol (Stevenage) 152:193–200
12. Bergendahl V, Glaser BT, Burgess RR (2003) A fast Western blot procedure improved for quantitative analysis by direct fl uorescence labeling of primary antibodies. J Immunol Methods 277:117–125
13. Bolt MW, Mahoney PA (1997) High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 247:185–192
14. Kashino Y, Koike H, Satoh K (2001) An improved sodium dodecyl sulfatepolyacrylamide gel electrophoresis system for the analysis of membrane protein complexes. Electrophoresis 22:1004–1007
15. Kurien BT, Scofi eld RH (2002) Heatmediated, ultra-rapid electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes. J Immunol Methods 266:127–133
16. Swank MW, Kumar V, Zhao J, Wu GY (2006) A novel method of loading samples onto minigels for SDS-PAGE: Increased sensitivity and Western blots using sub-microgram quantities of protein. J Neurosci Methods 158:224–33
17. Wu M, Stockley PG, Martin WJ 2nd (2002) An improved western blotting technique effectively reduces background. Electrophoresis 23:2373–2376
18. Schilling M, Maiwald T, Bohl S, Kollmann M, Kreutz C, Timmer J, Klingmuller U (2005) Computational processing and error reduction strategies for standardized quantitative data in biological networks. Febs J 272:6400–6411
19. Aksamitiene E, Hoek JB, Kholodenko B, Kiyatkin A (2007) Multistrip Western blotting to increase quantitative data output. Electrophoresis 28:3163–3173
20. Welinder C, Ekblad L (2011) Coomassie staining as loading control in Western blot analysis. J Proteome Res 10:1416–1419
21. Romero-Calvo I, Ocon B, Martinez-Moya P, Suarez MD, Zarzuelo A, Martinez-Augustin O, de Medina FS (2010) Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem 401:318–320
22. Aldridge GM, Podrebarac DM, Greenough WT, Weiler IJ (2008) The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. J Neurosci Methods 172:250–254
23. Kar P, Agnihotri SK, Sharma A, Sachan R, Lal Bhatt M, Sachdev M (2012) A protocol for stripping and reprobing of Western blots originally developed with colorimetric substrate TMB. Electrophoresis 33:3062–3065
24. Sennepin AD, Charpentier S, Normand T, Sarre C, Legrand A, Mollet LM (2009) Multiple reprobing of Western blots after inactivation of peroxidase activity by its substrate, hydrogen peroxide. Anal Biochem 393:129–131
25. Upadhaya R, Mizunoya W, Anderson JE (2011) Detecting multiple proteins by Western blotting using same-species primary antibodies, precomplexed serum, and hydrogen peroxide. Anal Biochem 419:342–344
26. Suzuki O, Koura M, Noguchi Y, Uchio- Yamada K, Matsuda J (2011) Use of sample mixtures for standard curve creation in quantitative western blots. Exp Anim 60:193–196
27. Dittmer A, Dittmer J (2006) Beta-actin is not a reliable loading control in Western blot analysis. Electrophoresis 27:2844–2845
28. Khimani AH, Mhashilkar AM, Mikulskis A, O’Malley M, Liao J, Golenko EE, Mayer P, Chada S, Killian JB, Lott ST (2005) Housekeeping genes in cancer: normalization of array data. Biotechniques 38:739–745
29. Janssens N, Janicot M, Perera T, Bakker A (2004) Housekeeping genes as internal standards in cancer research. Mol Diagn 8:107–113
30. Caradec J, Sirab N, Revaud D, Keumeugni C, Loric S (2010) Is GAPDH a relevant housekeeping gene for normalisation in colorectal cancer experiments? Br J Cancer 103:1475–1476
31. Waxman S, Wurmbach E (2007) De-regulation of common housekeeping genes in hepatocellular carcinoma. BMC Genomics 8:243
32. Rubie C, Kempf K, Hans J, Su T, Tilton B, Georg T, Brittner B, Ludwig B, Schilling M (2005) Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues. Mol Cell Probes 19:101–109
33. Sikand K, Singh J, Ebron JS, Shukla GC (2012) Housekeeping gene selection advisory: glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) and beta-actin are targets of miR- 644a. PLoS One 7:e47510
34. Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, Banks RE (2005) Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 5:566–571
35. Taylor SC, Berkelman T, Yadav G, Hammond M (2013) A defi ned methodology for reliable quantifi cation of Western blot data. Mol Biotechnol 55:217–226
36. Aksamitiene E, Achanta S, Kolch W, Kholodenko BN, Hoek JB, Kiyatkin A (2011) Prolactin-stimulated activation of ERK1/2 mitogen-activated protein kinases is controlled by PI3-kinase/Rac/PAK signaling pathway in breast cancer cells. Cell Signal 23:1794–1805
37. Simpson RJ (2010) Pouring linear gradient gels with a gradient former. Cold Spring Harbor Protoc. 2010: pdb prot5411