An improved western blotting technique effectively reduces background

Electrophoresis

Volumn 23, Issue 15, 2002, Pages 2373-2376

  Wu, Min ^a   Stockley, Peter G ^b   Martin II, William J ^a,c  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF LEEDS   (United Kingdom)

^c INDIANA UNIVERSITY   (United States)

Author keywords

Blackground reduction; Immunoblot; Polyacrylamide gel electrophoresis; Water washing

Indexed keywords

WASHING;

% REDUCTIONS; BLACKGROUND REDUCTION; BLOTTING ANALYSIS; CONDITION; IMMUNOBLOTS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SIMPLE METHOD; WASHING TIME; WATER WASHING; WESTERN-BLOTTING;

ELECTROPHORESIS;

BUFFER; REAGENT; WATER; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PROTEIN; VITRONECTIN;

ANALYTIC METHOD; ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; COST BENEFIT ANALYSIS; EXPERIMENTATION; LABOR; METHODOLOGY; PROTEIN ANALYSIS; TIME MANAGEMENT; WESTERN BLOTTING; ANIMAL; CELL LINE; CHEMISTRY; HUMAN; LUNG LAVAGE; MACROMOLECULE; RAT;

ANIMALS; BLOTTING, WESTERN; BRONCHOALVEOLAR LAVAGE FLUID; BUFFERS; CELL LINE; HUMANS; INDICATORS AND REAGENTS; MACROMOLECULAR SUBSTANCES; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MITOGEN-ACTIVATED PROTEIN KINASES; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PROTEINS; RATS; VITRONECTIN; WATER;

EID: 0036668043     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2683(200208)23:15<2373::AID-ELPS2373>3.0.CO;2-W     Document Type: Article

References (13)