Pouring linear gradient gels with a gradient former

Cold Spring Harbor Protocols

Volumn 5, Issue 4, 2010, Pages

  Simpson, Richard J ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLAMIDE; LINEAR GRADIENT GEL; MONOMER; N,N' METHYLENEBISACRYLAMIDE; POLYACRYLAMIDE GEL; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL ANALYSIS; CHEMICAL COMPOSITION; CLINICAL PROTOCOL; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CROSS LINKING; MOLECULAR WEIGHT; PARTICLE SIZE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS DESIGN; PROTEIN ANALYSIS; SEPARATION TECHNIQUE; STRUCTURE ACTIVITY RELATION;

EID: 77956290761     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5411     Document Type: Article

References (4)

1. Granzier HLM, Wang K. 1993. Gel electrophoresis of giant proteins: Solubilization and silver-staining of titin and nebulin from single muscle fiber segments. Electrophoresis 14: 56-64.
2. Schägger H, von Jagow GV. 1987. Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379.
3. Shi Q, Jackowski G. 1998. One-dimensional polyacrylamide gel electrophoresis. In Gel electrophoresis of proteins: A practical approach (ed. BD Hames), 3rd ed, pp. 1-52. IRL Press/Oxford University Press, New York.
4. Simpson RJ. 2006. SDS-PAGE of proteins. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4313.