Expression and characterization of geobacillus stearothermophilus sr74 recombinant α -amylase in pichia pastoris

BioMed Research International

Volumn 2015, Issue , 2015, Pages

  Gandhi, Sivasangkary ^a   Salleh, Abu Bakar ^a   Rahman, Raja Noor Zaliha Raja Abd ^a   Chor Leow, Thean ^a   Oslan, Siti Nurbaya ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; METHANOL; RECOMBINANT ALPHA AMYLASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ALCOHOL OXIDASE GENE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; GENE EXPRESSION SYSTEM; GENE SEQUENCE; GEOBACILLUS STEAROTHERMOPHILUS; KOMAGATAELLA PASTORIS; MOLECULAR CLONING; NONHUMAN; PH MEASUREMENT; PROTEIN EXPRESSION; TEMPERATURE MEASUREMENT; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; PICHIA;

GEOBACILLUS STEAROTHERMOPHILUS; PICHIA PASTORIS;

ALPHA-AMYLASES; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GEOBACILLUS STEAROTHERMOPHILUS; PICHIA; RECOMBINANT PROTEINS;

EID: 84930960061     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2015/529059     Document Type: Article

References (33)

1. C. O. Ibrahim,Development of applications of industrial enzymes from Malaysian indigenous microbial sources, Bioresource Technology, vol. 99, no. 11, pp. 4572-4582, 2008.
2. S. Janecek, B. Svensson, and E. A. MacGregor, α-amylase: an enzyme specificity found in various families of glycoside hydrolases, Cellular and Molecular Life Sciences, vol. 71, no. 7, pp. 1149-1170, 2014.
3. R. Gupta, P. Gigras, H. Mohapatra, V. K. Goswami, and B. Chauhan,Microbial α-amylases: a biotechnological perspective, Process Biochemistry, vol. 38, no. 11, pp. 1599-1616, 2003.
4. J. E.Nielsen and T.V. Borchert,Protein engineering of bacterial alphaα-amylases, Biochimica et Biophysica Acta-Protein Structure and Molecular Enzymology, vol. 1543, no. 2, pp. 253-274, 2000.
5. M. J. E. C. van der Maarel, B. van der Veen, J. C. M. Uitdehaag, H. Leemhuis, and L. Dijkhuizen,Properties and applications of starch-converting enzymes of the α-amylase family, Journal of Biotechnology, vol. 94, no. 2, pp. 137-155, 2002.
6. S. Sivaramakrishnan, D. Gangadharan, K. M. Nampoothiri, C. R. Soccol, and A. Pandey, α-Amylase from microbial sources-an overview on recent developments, Food Technology and Biotechnology, vol. 44, no. 2, pp. 173-184, 2006.
7. S. Das, S. Singh, V. Sharma, and M. L. Soni,Biotechnological applications of industrially important amylase enzyme, International Journal of Pharma and Bio Sciences, vol. 2, no. 1, pp. 486-496, 2011.
8. D. Divakaran, A. Chandran, and R. P. Chandran,Comparative study on production of α-amylase from Bacillus licheniformis strains, Brazilian Journal of Microbiology, vol. 42, no. 4, pp. 1397-1404, 2011.
9. Z.Al-Qodah,Production and characterization of thermostable α-amylase by thermophilic Geobacillus stearothermophilus, Biotechnology Journal, vol. 1, no. 7-8, pp. 850-857, 2006.
10. A. Espargaro, A. Villar-Pique, R. Sabate, and S. Ventura,Yeast prions form infectious amyloid inclusion bodies in bacteria, Microbial Cell Factories, vol. 11, article 89, 2012.
11. J. L. Cereghino and J. M. Cregg,Heterologous protein expression in the methylotrophic yeast Pichia pastoris, FEMS Microbiology Reviews, vol. 24, no. 1, pp. 45-66, 2000.
12. J. M. Cregg, J. L. Cereghino, J. Shi, andD. R.Higgins,Recombinantprotein expression inPichia pastoris, Applied Biochemistry and Biotechnology Part B: Molecular Biotechnology, vol. 16, no. 1, pp. 23-52, 2000.
13. G. P. L. Cereghino, J. L. Cereghino, C. Ilgen, and J. M. Cregg, "Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris, Current Opinion in Biotechnology, vol. 13, no. 4, pp. 329-332, 2002.
14. H.Marx, A. Mecklenbrauker, B. Gasser, M. Sauer, and D. Mattanovich, "Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus, FEMS Yeast Research, vol. 9, no. 8, pp. 1260-1270, 2009.
15. J.-G. Berrin, G. Williamson, A. Puigserver, J.-C. Chaix, W. R. McLauchlan, and N. Juge,High-level production of recombinant fungal endo-1,4-xylanase in the methylotrophic yeast Pichia pastoris, Protein Expression and Purification, vol. 19, no. 1, pp. 179-187, 2000.
16. D.Mattanovich,P.Branduardi, L. Dato,B.Gasser,M. Sauer, and D. Porro,Recombinant protein production in yeasts, Methods in Molecular Biology, vol. 824, pp. 329-358, 2012.
17. S.O. Ramchuran, B.Mateus, O. Holst, and E.N.Karlsson,The methylotrophic yeast Pichia pastoris as a host for the expression and production of thermostable xylanase from the bacterium Rhodothermus marinus, FEMS Yeast Research, vol. 5, no. 9, pp. 839-850, 2005.
18. P. Bernfeld,Amylases α-and α-methods, Enzymology, vol. 1, pp. 149-158, 1955.
19. M. M. Bradford,A rapid and sensitive method for the quantitation ofmicrogramquantities of protein utilizing the principle of protein dye binding, Analytical Biochemistry, vol. 72, no. 1-2, pp. 248-254, 1976.
20. U. K. Laemmli,Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, vol. 227, no. 5259, pp. 680-685, 1970.
21. M.Weidner,M. Taupp, and S. J. Hallam,Expression of recombinant proteins in themethylotrophic yeast Pichia pastoris, The Journal of VisualizedExperiments, no. 36, Article IDe1862, 2010.
22. H.Uehara, D. B. Choi, E.Y. Park, andM.Okabe,Expression of mouse α-amylase gene in methylotrophic yeast Pichia pastoris, Biotechnology and Bioprocess Engineering, vol. 5, no. 1, pp. 7-12, 2000.
23. A. A. Latiffi, A. B. Salleh, R. N. Z. R. A. Rahman, S. Nurbaya Oslan, and M. Basri,Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α-factor secretion signal in Pichia pastoris, Genes and Genetic Systems, vol. 88, no. 2, pp. 85-91, 2013.
24. L. Y. Ling, I. Ithoi, and F. M. Yik,Optimization for highlevel expression in Pichia pastoris and purification of truncated and full length recombinant sag2 of Toxoplasma gondii for diagnostic use, Southeast Asian Journal of Tropical Medicine and Public Health, vol. 41, no. 3, pp. 507-513, 2010.
25. J. M.Wu, J. C. Lin, L. L. Chieng, C. K. Lee, and T. A.Hsu,Combined use of GAP and AOX1 promoter to enhance the expression of human granulocyte-macrophage colony-stimulating factor in Pichia pastoris, Enzyme and Microbial Technology, vol. 33, no. 4, pp. 453-459, 2003.
26. B. Karakas, M. Inan, and M. Certel,Expression and characterization of Bacillus subtilis PY22 α-amylase in Pichia pastoris, Journal ofMolecular Catalysis B: Enzymatic, vol. 64, no. 3-4, pp. 129-134, 2010.
27. M. Irshad, Z. Anwar, M. Gulfraz, H. I. Butt, A. Ejaz, and H. Nawaz,Purification and characterization of α-amylase from Ganoderma tsuage growing in waste bread medium, African Journal of Biotechnology, vol. 11, no. 33, 2012.
28. M. Nirmala and G. Muralikrishna,Three α-amylases from malted finger millet (ragi, Eleusine coracana, Indaf-15)-purification and partial characterization, Phytochemistry, vol. 62, no. 1, pp. 21-30, 2003.
29. S. A. Fincan and B. Enez,Production, purification, and characterization of thermostable aα-amylase fromthermophilic Geobacillus stearothermophilus, Starch/Staerke, vol. 66, no. 1-2, pp. 182-189, 2014.
30. G. Aguilar, J. Morlon-Guyot, B. Trejo-Aguilar, and J. P. Guyot, "Purification and characterization of an extracellular α-amylase produced by Lactobacillus manihotivorans LMG 18010, an amylolytic lactic acid bacterium, Enzyme and Microbial Technology, vol. 27, no. 6, pp. 406-413, 2000.
31. K. J.Wanderley, F. A. G. Torres, L.M. P.Moraes, and C. J.Ulhoa, "Biochemical characterization of α-amylase from the yeast Cryptococcus flavus, FEMS Microbiology Letters, vol. 231, no. 2, pp. 165-169, 2004.
32. C.-H. Yang, Y.-C. Huang, C.-Y. Chen, and C.-Y.Wen,Expression of Thermobifida fusca thermostable raw starch digesting alphaα-amylase in Pichia pastoris and its application in raw sago starch hydrolysis, Journal of Industrial Microbiology and Biotechnology, vol. 37, no. 4, pp. 401-406, 2010.
33. A.Mahdavi, R.H. Sajedi,M. Rassa, andV. Jafarian,Characterization of an α-amylase with broad temperature activity froman acid-neutralizing Bacillus cereus strain, Iranian Journal of Biotechnology, vol. 8, no. 2, pp. 103-111, 2010.