Expression and characterization of Bacillus subtilis PY22 α-amylase in Pichia pastoris

Journal of Molecular Catalysis B: Enzymatic

Volumn 64, Issue 3-4, 2010, Pages 129-134

  Karakaş, Barçin ^a   Inan, Mehmet ^a   Certel, Muharrem ^b  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b AKDENIZ UNIVERSITY   (Turkey)

Author keywords

Amylase; Bacillus subtilis PY22; Characterization; Overexpression; Pichia pastoris

Indexed keywords

AMYLASE; AMYLASE ACTIVITY; AMYLASE PRODUCTION; BACILLUS SUBTILIS; COPY NUMBER; DEGRADING ENZYMES; EXTRACELLULAR; HOST STRAIN; OVER-EXPRESSION; P. PASTORIS; PARTIAL PURIFICATION; PICHIA PASTORIS; PURIFIED ENZYME; REACTION MEDIUM; RECOMBINANT ENZYMES; RECOMBINANT EXPRESSION; SHAKE FLASKS; SHAKE-FLASK CULTURES; SMALL-SCALE PRODUCTION;

BACTERIOLOGY; CALCIUM; CLONING; ENZYMES; GENE ENCODING; GENE EXPRESSION; METHANOL; PURIFICATION;

ENZYME ACTIVITY;

AMYLASE; BACTERIAL ENZYME; CALCIUM ION; RECOMBINANT ENZYME;

ARTICLE; BACILLUS SUBTILIS; CELL LEVEL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; FUNGAL STRAIN; GENE AMPLIFICATION; GENE NUMBER; GENETIC SCREENING; MOLECULAR CLONING; NONHUMAN; PH; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN SECRETION; SEQUENCE ANALYSIS; TEMPERATURE;

BACILLUS SUBTILIS; PICHIA PASTORIS;

EID: 77951977402     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2009.07.006     Document Type: Article

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