Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris

Journal of Visualized Experiments

Volumn , Issue 36, 2010, Pages

  Weidner, Maria ^a   Taupp, Marcus ^a   Hallam, Steven J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Issue 36; Methylotrophic; Microbiology; Pichia pastoris; Protein expression; Protein purification; Recombinant protein; Western blot; Yeast; Yeast DNA purification

Indexed keywords

EID: 80055114715     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1862     Document Type: Article

References (4)

1. Cereghino, G.P., Cregg, J.M. Applications of yeast in biotechnology: protein production and genetic analysis. Curr Opin Biotechnol 10, 422-427 (1999).
2. Cregg, J. M., Barringer, K. J., Hessler, A. Y. Pichia pastoris as a Host System for Transformations. Mol. Cell. Biol. 5, 3376-3385 (1985).
3. Cregg, J. M., Cereghino J. L., Shi J., Higgins D.R. Recombinant protein expression in Pichia pastoris. Mol Biotechnol. 16, 23-52 (2000).
4. Cereghino, G.P., Cereghino J.L., Ilgen C., Cregg J.M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 4, 329-32 (2002)