The Ran-GTP gradient spatially regulates XCTK2 in the spindle

Current Biology

Volumn 25, Issue 11, 2015, Pages 1509-1514

  Weaver, Lesley N ^a   Ems Mcclung, Stephanie C ^a   Chen, Sez Hon R ^b   Yang, Ge ^b   Shaw, Sidney L ^a   Walczak, Claire E ^a  

^a INDIANA UNIVERSITY   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATE; KARYOPHERIN; KINESIN; RAN PROTEIN; XCTK2 PROTEIN, XENOPUS; XENOPUS PROTEIN;

ANIMAL; CELL LINE; METABOLISM; SPINDLE APPARATUS; SPODOPTERA; XENOPUS;

ANIMALS; CELL LINE; GUANOSINE TRIPHOSPHATE; KARYOPHERINS; KINESIN; RAN GTP-BINDING PROTEIN; SPINDLE APPARATUS; SPODOPTERA; XENOPUS; XENOPUS PROTEINS;

EID: 84930928467     PISSN: 09609822     EISSN: 18790445     Source Type: Journal    
DOI: 10.1016/j.cub.2015.04.015     Document Type: Article

References (33)

1. P.R. Clarke, and C. Zhang Spatial and temporal coordination of mitosis by Ran GTPase Nat. Rev. Mol. Cell Biol. 9 2008 464 477
2. R.E. Carazo-Salas, G. Guarguaglini, O.J. Gruss, A. Segref, E. Karsenti, and I.W. Mattaj Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation Nature 400 1999 178 181
3. P. Kalab, R.T. Pu, and M. Dasso The Ran GTPase regulates mitotic spindle assembly Curr. Biol. 9 1999 481 484
4. T. Ohba, M. Nakamura, H. Nishitani, and T. Nishimoto Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran Science 284 1999 1356 1358
5. A. Wilde, and Y. Zheng Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran Science 284 1999 1359 1362
6. P. Kalab, K. Weis, and R. Heald Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts Science 295 2002 2452 2456
7. O.J. Gruss, R.E. Carazo-Salas, C.A. Schatz, G. Guarguaglini, J. Kast, M. Wilm, N. Le Bot, I. Vernos, E. Karsenti, and I.W. Mattaj Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity Cell 104 2001 83 93
8. M.V. Nachury, T.J. Maresca, W.C. Salmon, C.M. Waterman-Storer, R. Heald, and K. Weis Importin β is a mitotic target of the small GTPase Ran in spindle assembly Cell 104 2001 95 106
9. C. Wiese, A. Wilde, M.S. Moore, S.A. Adam, A. Merdes, and Y. Zheng Role of importin-β in coupling Ran to downstream targets in microtubule assembly Science 291 2001 653 656
10. T. Wittmann, M. Wilm, E. Karsenti, and I. Vernos TPX2, A novel Xenopus MAP involved in spindle pole organization J. Cell Biol. 149 2000 1405 1418
11. M.D. Koffa, C.M. Casanova, R. Santarella, T. Köcher, M. Wilm, and I.W. Mattaj HURP is part of a Ran-dependent complex involved in spindle formation Curr. Biol. 16 2006 743 754
12. H.H. Silljé, S. Nagel, R. Körner, and E.A. Nigg HURP is a Ran-importin beta-regulated protein that stabilizes kinetochore microtubules in the vicinity of chromosomes Curr. Biol. 16 2006 731 742
13. N. Trieselmann, S. Armstrong, J. Rauw, and A. Wilde Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation J. Cell Sci. 116 2003 4791 4798
14. S.C. Ems-McClung, Y. Zheng, and C.E. Walczak Importin α/β and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal Mol. Biol. Cell 15 2004 46 57
15. G. Goshima, F. Nédélec, and R.D. Vale Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins J. Cell Biol. 171 2005 229 240
16. M. Hatsumi, and S.A. Endow Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis J. Cell Sci. 101 1992 547 559
17. J. Matuliene, R. Essner, J. Ryu, Y. Hamaguchi, P.W. Baas, T. Haraguchi, Y. Hiraoka, and R. Kuriyama Function of a minus-end-directed kinesin-like motor protein in mammalian cells J. Cell Sci. 112 1999 4041 4050
18. V. Mountain, C. Simerly, L. Howard, A. Ando, G. Schatten, and D.A. Compton The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle J. Cell Biol. 147 1999 351 366
19. D.J. Sharp, K.R. Yu, J.C. Sisson, W. Sullivan, and J.M. Scholey Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos Nat. Cell Biol. 1 1999 51 54
20. C.E. Walczak, S. Verma, and T.J. Mitchison XCTK2: a kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts J. Cell Biol. 136 1997 859 870
21. S. Cai, L.N. Weaver, S.C. Ems-McClung, and C.E. Walczak Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules Mol. Biol. Cell 20 2009 1348 1359
22. F.R. Bischoff, C. Klebe, J. Kretschmer, A. Wittinghofer, and H. Ponstingl RanGAP1 induces GTPase activity of nuclear Ras-related Ran Proc. Natl. Acad. Sci. USA 91 1994 2587 2591
23. M. Dasso, T. Seki, Y. Azuma, T. Ohba, and T. Nishimoto A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation EMBO J. 13 1994 5732 5744
24. T.J. Maresca, A.C. Groen, J.C. Gatlin, R. Ohi, T.J. Mitchison, and E.D. Salmon Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity Curr. Biol. 19 2009 1210 1215
25. G. Yang, L.A. Cameron, P.S. Maddox, E.D. Salmon, and G. Danuser Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle J. Cell Biol. 182 2008 631 639
26. K. Furuta, and Y.Y. Toyoshima Minus-end-directed motor Ncd exhibits processive movement that is enhanced by microtubule bundling in vitro Curr. Biol. 18 2008 152 157
27. R. Chandra, E.D. Salmon, H.P. Erickson, A. Lockhart, and S.A. Endow Structural and functional domains of the Drosophila ncd microtubule motor protein J. Biol. Chem. 268 1993 9005 9013
28. A. Karabay, and R.A. Walker Identification of microtubule binding sites in the Ncd tail domain Biochemistry 38 1999 1838 1849
29. R.A. Walker Ncd and kinesin motor domains interact with both alpha- and beta-tubulin Proc. Natl. Acad. Sci. USA 92 1995 5960 5964
30. L. Tao, A. Mogilner, G. Civelekoglu-Scholey, R. Wollman, J. Evans, H. Stahlberg, and J.M. Scholey A homotetrameric kinesin-5, KLP61F, bundles microtubules and antagonizes Ncd in motility assays Curr. Biol. 16 2006 2293 2302
31. M. Braun, D.R. Drummond, R.A. Cross, and A.D. McAinsh The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism Nat. Cell Biol. 11 2009 724 730
32. G. Fink, L. Hajdo, K.J. Skowronek, C. Reuther, A.A. Kasprzak, and S. Diez The mitotic kinesin-14 Ncd drives directional microtubule-microtubule sliding Nat. Cell Biol. 11 2009 717 723
33. C. Hentrich, and T. Surrey Microtubule organization by the antagonistic mitotic motors kinesin-5 and kinesin-14 J. Cell Biol. 189 2010 465 480