HURP Is a Ran-Importin β-Regulated Protein that Stabilizes Kinetochore Microtubules in the Vicinity of Chromosomes

Current Biology

Volumn 16, Issue 8, 2006, Pages 731-742

  Silljé, Herman H W ^a   Nagel, Susanna ^a   Körner, Roman ^a   Nigg, Erich A ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

CELLBIO

Indexed keywords

DLG7 PROTEIN, HUMAN; KARYOPHERIN BETA; RAN PROTEIN; TUMOR PROTEIN;

ARTICLE; CELL NUCLEUS; CENTROMERE; CHROMOSOME; CYTOPLASM; HELA CELL; HUMAN; METABOLISM; MICROTUBULE; MITOSIS SPINDLE; PHYSIOLOGY;

BETA KARYOPHERINS; CELL NUCLEUS; CHROMOSOMES; CYTOPLASM; HELA CELLS; HUMANS; KINETOCHORES; MICROTUBULES; MITOTIC SPINDLE APPARATUS; NEOPLASM PROTEINS; RAN GTP-BINDING PROTEIN;

EID: 33646061361     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cub.2006.02.070     Document Type: Article

References (43)

1. McEwen B.F., Heagle A.B., Cassels G.O., Buttle K.F., and Rieder C.L. Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset. J. Cell Biol. 137 (1997) 1567-1580
2. Rieder C.L., and Salmon E.D. The vertebrate cell kinetochore and its roles during mitosis. Trends Cell Biol. 8 (1998) 310-318
3. Kline-Smith S.L., and Walczak C.E. Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics. Mol. Cell 15 (2004) 317-327
4. Kirschner M., and Mitchison T. Beyond self-assembly: from microtubules to morphogenesis. Cell 45 (1986) 329-342
5. Heald R., Tournebize R., Habermann A., Karsenti E., and Hyman A. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 138 (1997) 615-628
6. Heald R., and Weis K. Spindles get the ran around. Trends Cell Biol. 10 (2000) 1-4
7. Dasso M. The Ran GTPase: theme and variations. Curr. Biol. 12 (2002) R502-R508
8. Gruss O.J., and Vernos I. The mechanism of spindle assembly: functions of Ran and its target TPX2. J. Cell Biol. 166 (2004) 949-955
9. Rieder C.L. Kinetochore fiber formation in animal somatic cells: dueling mechanisms come to a draw. Chromosoma 114 (2005) 310-318
10. Caudron M., Bunt G., Bastiaens P., and Karsenti E. Spatial coordination of spindle assembly by chromosome-mediated signaling gradients. Science 309 (2005) 1373-1376
11. Kalab P., Weis K., and Heald R. Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science 295 (2002) 2452-2456
12. Li H.Y., and Zheng Y. Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells. Genes Dev. 18 (2004) 512-527
13. Gruss O.J., Carazo-Salas R.E., Schatz C.A., Guarguaglini G., Kast J., Wilm M., Le Bot N., Vernos I., Karsenti E., and Mattaj I.J. Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity. Cell 104 (2001) 83-93
14. Wiese C., Wilde A., Moore M.S., Adam S.A., Merdes A., and Zheng Y. Role of importin-beta in coupling Ran to downstream targets in microtubule assembly. Science 291 (2001) 653-656
15. Nachury M.V., Maresca T.J., Salmon W.C., Waterman-Storer C.M., Heald R., and Weis K. Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell 104 (2001) 95-106
16. Ems-McClung S.C., Zheng Y., and Walczak C.E. Importin alpha/beta and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal. Mol. Biol. Cell 15 (2004) 46-57
17. Maresca T.J., Niederstrasser H., Weis K., and Heald R. Xnf7 contributes to spindle integrity through its microtubule-bundling activity. Curr. Biol. 15 (2005) 1755-1761
18. Blower M.D., Nachury M., Heald R., and Weis K. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell 121 (2005) 223-234
19. Du Q., Taylor L., Compton D.A., and Macara I.G. LGN blocks the ability of NuMA to bind and stabilize microtubules. A mechanism for mitotic spindle assembly regulation. Curr. Biol. 12 (2002) 1928-1933
20. Khodjakov A., Cole R.W., Oakley B.R., and Rieder C.L. Centrosome-independent mitotic spindle formation in vertebrates. Curr. Biol. 10 (2000) 59-67
21. Hinchcliffe E.H., Miller F.J., Cham M., Khodjakov A., and Sluder G. Requirement of a centrosomal activity for cell cycle progression through G1 into S phase. Science 291 (2001) 1547-1550
22. Bonaccorsi S., Giansanti M.G., and Gatti M. Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J. Cell Biol. 142 (1998) 751-761
23. Megraw T.L., Kao L.R., and Kaufman T.C. Zygotic development without functional mitotic centrosomes. Curr. Biol. 11 (2001) 116-120
24. Maiato H., Rieder C.L., and Khodjakov A. Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis. J. Cell Biol. 167 (2004) 831-840
25. Khodjakov A., Copenagle L., Gordon M.B., Compton D.A., and Kapoor T.M. Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis. J. Cell Biol. 160 (2003) 671-683
26. Sauer G., Korner R., Hanisch A., Ries A., Nigg E.A., and Sillje H.H. Proteome analysis of the human mitotic spindle. Mol. Cell. Proteomics 4 (2005) 35-43
27. Tsou A.P., Yang C.W., Huang C.Y., Yu R.C., Lee Y.C., Chang C.W., Chen B.R., Chung Y.F., Fann M.J., Chi C.W., et al. Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. Oncogene 22 (2003) 298-307
28. Hsu J.M., Lee Y.C., Yu C.T., and Huang C.Y. Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region. J. Biol. Chem. 279 (2004) 32592-32602
29. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
30. Rieder C.L. The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells. Chromosoma 84 (1981) 145-158
31. Nishi K., Yoshida M., Fujiwara D., Nishikawa M., Horinouchi S., and Beppu T. Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression. J. Biol. Chem. 269 (1994) 6320-6324
32. Ren M., Drivas G., D'Eustachio P., and Rush M.G. Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis. J. Cell Biol. 120 (1993) 313-323
33. Bischoff F.R., Klebe C., Kretschmer J., Wittinghofer A., and Ponstingl H. RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Natl. Acad. Sci. USA 91 (1994) 2587-2591
34. Klebe C., Bischoff F.R., Ponstingl H., and Wittinghofer A. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1. Biochemistry 34 (1995) 639-647
35. Dasso M., Seki T., Azuma Y., Ohba T., and Nishimoto T. A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation. EMBO J. 13 (1994) 5732-5744
36. Nishitani H., Ohtsubo M., Yamashita K., Iida H., Pines J., Yasudo H., Shibata Y., Hunter T., and Nishimoto T. Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis. EMBO J. 10 (1991) 1555-1564
37. Wittmann T., Wilm M., Karsenti E., and Vernos I. TPX2, a novel Xenopus MAP involved in spindle pole organization. J. Cell Biol. 149 (2000) 1405-1418
38. Gruss O.J., Wittmann M., Yokoyama H., Pepperkok R., Kufer T., Sillje H., Karsenti E., Mattaj I.W., and Vernos I. Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nat. Cell Biol. 4 (2002) 871-879
39. Yu C.T., Hsu J.M., Lee Y.C., Tsou A.P., Chou C.K., and Huang C.Y. Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A. Mol. Cell. Biol. 25 (2005) 5789-5800
40. Bischoff J.R., Anderson L., Zhu Y., Mossie K., Ng L., Souza B., Schryver B., Flanagan P., Clairvoyant F., Ginther C., et al. A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J. 17 (1998) 3052-3065
41. Kufer T.A., Nigg E.A., and Sillje H.H. Regulation of Aurora-A kinase on the mitotic spindle. Chromosoma 112 (2003) 159-163
42. Arnaoutov A., Azuma Y., Ribbeck K., Joseph J., Boyarchuk Y., Karpova T., McNally J., and Dasso M. Crm1 is a mitotic effector of Ran-GTP in somatic cells. Nat. Cell Biol. 7 (2005) 626-632
43. Salina D., Enarson P., Rattner J.B., and Burke B. Nup358 integrates nuclear envelope breakdown with kinetochore assembly. J. Cell Biol. 162 (2003) 991-1001