In vitro genotoxicity testing of carvacrol and thymol using the micronucleus and mouse lymphoma assays

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 784-785, Issue , 2015, Pages 37-44

  Maisanaba, Sara ^a   Prieto, Ana I ^a   Puerto, Maria ^a   Gutiérrez Praena, Daniel ^a   Demir, Eşref ^b   Marcos, Ricard ^c,d   Cameán, Ana M ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

^b AKDENIZ UNIVERSITY   (Turkey)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^d CIBER EPIDEMIOLOGÍA Y SALUD PÚBLICA CIBERESP   (Spain)

Author keywords

Carvacrol; Food packaging; Micronucleus test; Mouse lymphoma assay; Thymol

Indexed keywords

CARVACROL; CYCLOPHOSPHAMIDE; MITOMYCIN; THYMOL; DNA; FOOD ADDITIVE; TERPENE;

ANIMAL CELL; ARTICLE; BIOAVAILABILITY; CONTROLLED STUDY; CYTOTOXICITY TEST; FOOD PACKAGING; GENOTOXICITY; GENOTOXICITY ASSAY; HEALTH HAZARD; IN VITRO STUDY; LYMPHOMA; LYMPHOMA CELL; MAMMAL CELL; METABOLIC ACTIVATION; MICRONUCLEUS; MICRONUCLEUS TEST; MOUSE; MOUSE LYMPHOMA ASSAY; MUTAGENICITY; NONHUMAN; OREGANO; PACKAGING MATERIAL; PRIORITY JOURNAL; TOXICITY TESTING; ANIMAL; DNA DAMAGE; DOSE RESPONSE; DRUG EFFECTS; L5178Y CELL LINE; TUMOR CELL LINE;

MAMMALIA; ORIGANUM VULGARE;

ANIMALS; CELL LINE, TUMOR; DNA DAMAGE; DNA, NEOPLASM; DOSE-RESPONSE RELATIONSHIP, DRUG; FOOD ADDITIVES; IN VITRO TECHNIQUES; LEUKEMIA L5178; MICE; MICRONUCLEUS TESTS; MONOTERPENES; THYMOL;

EID: 84930274628     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2015.05.005     Document Type: Article

References (40)

1. Bajpai V.K., Baek K.H., Kang S.C. Control of Salmonella in foods by using essential oils: a review. Food Res. Int. 2012, 45:722-734.
2. Teixeira B., Marques A., Ramos C., Batista I., Serrano C., Matos O., Neng N.R., Nogueira J.M.F., Saraiva J.A., Nunes M.L. European pennyroyal (Menthapulegium) from Portugal: chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil arbara. Ind. Crops Prod. 2012, 36:81-87.
3. Roby M.H.H., Sarhana M.A., Selima K.A.H., Khalela K.I. Evaluation of antioxidant activity total phenols and phenolic compounds in thyme (Thymus vulgaris L.) sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Ind. Crops Prod. 2013, 43:827-831.
4. Misharina T.A., Alinkina E.S., Medvedeva I.B. Antiradical properties of essential oils and extracts from clove bud and pimento. Appl. Biochem. Microbiol. 2015, 51:119-124.
5. Hyldgaard M., Mygind T., Meyer R.L. Essential oils in food preservation: mode of action, synergies, and interaction with food matrix components. Front. Microbiol. 2012, 3:1-24.
6. Nerín C., Tovar L., Djenane D., Camo J., Salafranca J., Beltrán J.A., Roncalés P. Stabilization of beef meat by a new active packaging containing natural antioxidants. J. Agric. Food Chem. 2006, 54:7840-7846.
7. Kuorwel K., Cran M.J., Sonneveld K., Miltz J., Bigger S.W. Antimicrobial activity of biodegradable polysaccharide and protein-based films containing active agents. J. Food Sci. 2011, 76:90-102.
8. Ramos M., Jimenez A., Peltzer M., Garrigos M.C. Characterization and antimicrobial activity studies of polypropylene films with carvacrol and thymol for active packaging. J. Food Eng. 2012, 109:513-519.
9. Seow Y.X., Yeo C.R., Chung H.L., Yuk H.G. Plant essential oils as active antimicrobial agents. Crit. Rev. Food. Sci. Nutr. 2014, 54:625-644.
10. Bakkali F., Averbeck S., Averbeck D., Idaomar M. Biological effects of essential oils - a review. Food Chem. Toxicol. 2008, 46:446-475.
11. European Food Safety Authority Scientific opinion on genotoxicity testing strategies applicable to food and feed safety assessment. EFSA J. 2011, 9(9):1-69. arxiv:/www.efsa.europa.eu/efsajournal, 10.2903/j.efsa.2011.2379.
12. Kirkland E., Zeiger F., Madia N., Gooderham P., Kasper A., Lynch T., Morita G., Parra Morte S., Pfuhler V., Rogiers M., Schulz V., van Benthem P., Vanparys A., Corvi R. Can in vitro mammalian cell genotoxicity test results be used to complement positive results in the Ames test and help predict carcinogenic or in vivo genotoxic activity? I. Reports of individual data bases presented at an EURL ECVAM workshop. Mutat. Res. 2014, 775-776:55-68.
13. European Commission, 2001. Guidelines of the Scientific Committee on Food for the presentation of an application for safety assessment of a substance to be used in food contact materials prior to its authorization.
14. Moore M.M., Honna M., Clements J., Bolcsfoldi G., Cifone M., Delongchamp R., Fellows M., Gollapudi B., Jenkinson P., Kirby P., Kirchner S., Muster W., Myhre B., O'donovan M., Oliver J., Omori T., Ouldelhkimm M.C., Pant K., Preston R., Riach C., San R., Stankowski L.F.E., Thakur A., Wakuri S., Hosimura I. Mouse lymphoma thymidine kinase gene mutation assay: international workshop on geneotoxicity tests workgroup report- plymouth, UK 2002. Mutat. Res. 2003, 540:127-140.
15. Di Sotto A., Maffei F., Hrelia P., Castelli F., Sarpietro M.G., Mazzanti G. Genotoxicity assessment of β-caryophyllene oxide. Regul. Toxicol. Pharm. 2013, 66:264-268.
16. Liju V.B., Jeena K., Kuttan R. Acute and subchronic toxicity as well as mutagenic evaluation of essential oil from turmeric (Curcuma longa L.). Food Chem. Toxicol. 2013, 53:52-61.
17. Nicolella H.D., De Oliveira P.F., Munari C.C., Dias Costa G.F., Rodrigues Moreira M., Veneziani R.C.S., Tavares D.C. Differential effect of manool - a diterpene from Salvia officinalis, on genotoxicity induced by methyl methanesulfonate in V79 and HepG2 cells. Food Chem. Toxicol. 2014, 72:8-12.
18. Lorge E., Thybaud V., Aardemac M.J., Oliver J., Wakata A., Lorenzon G., Marzin D. SFTG international collaborative study on in vitro micronucleus test I. General conditions and overall conclusions of the study. Mutat. Res. 2006, 607:13-36.
19. Hamedo H., Abdelmigid H.M. Use of antimicrobial and genotoxicity potentially for evaluation of essentials oils as food preservative. Open Biotechnol. J. 2009, 3:50-56.
20. Hovhannisyan G.G. Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology. Mol. Cytogenet. 2010, 3:17.
21. Aydin S., Basaran A.A., Basaran N. The effects of thyme volatiles on the induction of DNA damage by the heterocyclic amine IQ and mitomycin C. Mutat. Res. 2005, 581:43-53.
22. Aydin S., Basaran A.A., Basaran N. Modulating effects of thyme and its major ingredients on oxidative DNA damage in human lymphocytes. J. Agric. Food Chem. 2005, 53:1299-1305.
23. Horvathova E., Sramkova M., Labaj J., Slamenova D. Study of cytotoxic, genotoxic and DNA-protective effects of selected plant essential oils on human cells cultured in vitro. Neuro. Endocrinol. Lett. 2006, 27:44-47.
24. Slamenova D., Horvathova E., Sramkova M., Marsalkova L. DNA-protective effects of two components of essential plant oils carvacrol and thymol on mammalian cells cultured in vitro. Neoplasma 2007, 54:108-112.
25. Buyukleyla M., Rencuzogullari E. The effects of thymol on sister chromatid exchange, chromosome aberration and micronucleus in human lymphocytes. Ecotox. Environ. Safe. 2009, 72:943-947.
26. Ündeger U., Basaran A., Degen G.H., Basaran N. Antioxidant activities of major thyme ingredients and lack of (oxidative) DNA damage in V79 Chinese hamster lung fibroblast cells at low levels of carvacrol and thymol. Food Chem. Toxicol. 2009, 47:2037-2043.
27. Llana-Ruiz-Cabello M., Maisanaba S., Puerto M., Prieto A.I., Pichardo S., Jos A., Cameán A.M. Evaluation of the mutagenicity and genotoxic potential of carvacrol and thymol using the Ames Salmonella test and alkaline, Endo III- and FPG-modified comet assays with the human cell line Caco-2. Food Chem. Toxicol. 2014, 72:123-128.
28. Stammati A., Bonsi P., Zucco F., Moezelaar R., Alakomi H.L., von Wright A. Toxicity of selected plant volatiles in microbial and mammalian short-term assays. Food Chem. Toxicol. 1999, 37:813-823.
29. Ipek E., Zeytinoglu H., Okay S., Tuylu B.A., Kurkcuoglu M., Can Baser K.H. Genototoxicity and antigenotoxicity of Origanum oil and carvacrol evaluated by Ames Salmonella/microsomal test. Food Chem. 2005, 93:551-556.
30. Llana-Ruiz-Cabello M., Gutierrez-Praena D., Pichardo S., Moreno F.J., Bermudez J.M., Aucejo S., Cameán A.M. Cytotoxicity and morphological effects induced by carvacrol and thymol on the human cell line Caco-2. Food Chem. Toxicol. 2014, 64:281-290.
31. OECD, 2010. Guideline for the testing of chemicals 487: In vitro mammalian cell micronucleus test. Pp 1-23.
32. W. Strober, Trypan blue exclusion test of cell viability, Curr. Protoc. Immunol. (2001) Appendix 3: Appendix 3B.
33. Fenech M. The in vitro micronucleus technique. Mutat. Res. 2000, 455:81-95.
34. Soriano C., Creus A., Marcos R. Gene-mutation induction by arsenic compounds in the mouse lymphoma assay. Mut. Res. 2007, 634:40-50.
35. ICH Expert Working Group, International conferences on harmonization of technical requirements for registration of pharmaceuticals for human use, in: ICH Harmonised Tripartite Guideline Guidance on Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use. Draft January 28th (2008) version 5.3.
36. Honma M., Hayashi M., Shimada H., Tanaka N., Wakuri S., Awogi T., Yamamoto K.I., Kodani N., Nishi Y., Nakadate M., Sofuni T. Evaluation of the mouselymphomaTk assay (microwell method) as an alternative to the in vitro chromosomal aberration test. Mutagenesis 1999, 14:5-22.
37. Azizan A., Blevins R.D. Mutagenicity and antimutagenicity testing of six chemicals associated with the pungent properties of specific spices as revealed by the Ames Salmonella/microsomal assay. Arch. Environ. Contam. Toxicol. 1995, 28:248-258.
38. 2): the influence of carvacrol. Mutat. Res. 2013, 757:15-22.
39. Vicuña G.C., Stashenko E.E., Fuentes J.L. Chemical composition of the Lippiaoriganoides essential oils and their antigenotoxicity against bleomycin-induced DNA damage. Fitoterapia 2010, 81(2010):343-349.
40. Aydin E., Türkez H., Keles M.S. The effect of carvacrol on healthy neurons and N2a cancer cells: some biochemical: anticancerogenicity and genotoxicity studies. Cytotechnology 2014, 66:149-157.