Comparison of biological processes induced in HepG2 cells by tert-butyl hydroperoxide (t-BHP) and hydroperoxide (H2O2): The influence of carvacrol

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 757, Issue 1, 2013, Pages 15-22

  Slamenova, Darina ^a   Kozics, Katarina ^a   Hunakova, Lubica ^a   Melusova, Martina ^a,b   Navarova, Jana ^c   Horvathova, Eva ^a  

^a CANCER RESEARCH INSTITUTE   (Slovakia)

^b SLOVAK UNIVERSITY OF TECHNOLOGY   (Slovakia)

^c INSTITUTE OF EXPERIMENTAL PHARMACOLOGY AND TOXICOLOGY   (Slovakia)

Author keywords

Antioxidant enzymes; Carvacrol; DNA lesions; Human hepatocellular carcinoma cells; Hydrogen peroxide; Reactive oxidative species; tert Butyl hydroperoxide

Indexed keywords

ALKALI; CARVACROL; DNA GLYCOSYLTRANSFERASE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; HYDROPEROXIDE; PYRIMIDINE; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE; TERT BUTYL HYDROPEROXIDE; HYDROGEN PEROXIDE; TERPENE;

ANTIOXIDANT ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; CANCER CELL DESTRUCTION; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; ENZYME ACTIVITY; GENOTOXICITY; HEP-G2 CELL LINE; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SINGLE STRANDED DNA BREAK; DRUG EFFECTS; HEPG2 CELL LINE; METABOLISM; TOXICITY;

DNA BREAKS, SINGLE-STRANDED; GLUTATHIONE; GLUTATHIONE PEROXIDASE; HEP G2 CELLS; HUMANS; HYDROGEN PEROXIDE; MONOTERPENES; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; SUPEROXIDE DISMUTASE; TERT-BUTYLHYDROPEROXIDE;

EID: 84891553446     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2013.03.014     Document Type: Article

References (46)

1. Seifried, H.E., Anderson, D.E., Fisher, E.I., Milner, J.A., A review of the interaction among dietary antioxidants and reactive oxygen species. J. Nutr. Biochem. 18 (2007), 567–579.
2. Castro, L., Freeman, B.A., Reactive oxygen species in human health and disease. Nutrition 17 (2001), 163–165.
3. Halliwell, B., Aruoma, O.I., DNA damage by oxygen-derived species: its mechanism and measurement in mammalian systems. FEBS Lett. 281 (1991), 9–19.
4. Halliwell, B., Free radicals and antioxidants: updating a personal view. Nutr. Rev. 70 (2012), 257–265.
5. Kennedy, C.H., Church, D.F., Winston, G.W., Pryor, W.A., tert-Butyl hydroperoxide-induced radical production in rat liver mitochondria. Free Radic. Biol. Med. 12 (1992), 381–387.
6. Latour, I., Demoulin, J.B., Buc-Calderon, P., Oxidative DNA damage by t-butyl hydroperoxide causes DNA single strand breaks which is not linked to cell lyses: a mechanistic study in freshly isolated rat hepatocytes. FEBS Lett. 373 (1995), 299–302.
7. Guidarelli, A., Clementi, E., Sciorati, C., Cattabeni, F., Cantoni, O., Calcium-dependent mitochondrial formation of species mediating DNA single strand breakage in U937 cells exposed to sublethal concentrations of tert-butyl hydroperoxide. J. Pharmacol. Exp. Ther. 283 (1997), 66–74.
8. Barry, M.A., Eastman, A., Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch. Biochem. Biophys. 300 (1993), 440–450.
9. Sakaida, I., Thomas, A.P., Farber, J.L., Increases in cytosolic calcium ion concentration can be dissociated from the killing of cultured hepatocytes by tert-butyl hydroperoxide. J. Biol. Chem. 266 (1991), 717–722.
10. Martin, C., Martinez, R., Navarro, R., Ruiz-Sanz, J.I., Lacort, M., Ruiz-Larrea, M.B., tert-Butyl hydroperoxide-induced lipid signaling in hepatocytes: involvement of glutathione and free radicals. Biochem. Pharmacol. 62 (2001), 705–712.
11. Sarkar, M.K., Sil, P.C., Prevention of tertiary butyl hydroperoxide-induced oxidative impairment and cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. Toxicol. In Vitro 24 (2010), 1711–1719.
12. 2 and tertiary butyl hydroperoxide upon a murine immortal lens epithelial cell line αTN4-1. Exp. Eye Res. 75 (2002), 573–582.
13. Droge, W., Free radicals in the physiological control of cell function. Physiol. Rev. 82 (2002), 47–95.
14. Stanner, S.A., Hughes, J., Kelly, C.N., Buttriss, J., A review of the epidemiological evidence for the antioxidant hypothesis. Public Health Nutr. 7 (2004), 407–422.
15. Aeschbach, R., Loliger, J., Scott, B.C., Murcia, A., Butler, J., Halliwell, B., Aruoma, O.I., Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food Chem. Toxicol. 32 (1994), 31–36.
16. Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L., A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988), 184–191.
17. Slamenova, D., Gabelova, A., Ruzekova, L., Chalupa, I., Horvathova, E., Farkasova, T., Bozsakyova, E., Stetina, R., Detection of MNNG-induced DNA lesions in mammalian cells: validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations. Mutat. Res. 383 (1997), 243–252.
18. Collins, A.R., Duthie, S.J., Dobson, V.L., Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis 14 (1993), 1733–1735.
19. Von Gadow, A., Joubert, E., Hansmann, Ch.F., Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), α-tocopherol, BHT, and BHA. J. Agric. Food Chem. 45 (1997), 632–638.
20. Buricova, L., Reblova, Z., Czech medicinal plants as possible sources of antioxidants. Czech J. Food Sci. 26 (2008), 132–138.
21. Ramos, A.A., Lima, C.F., Pereira, M.L., Fernandes-Ferreira, M., Pereira-Wilson, C., Antigenotoxic effects of quercetin, rutin and ursolic acid on HepG2 cells; evaluation by the comet assay. Toxicol. Lett. 177 (2008), 66–73.
22. Gabelova, A., Slamenova, D., Ruzekova, L., Farkasova, T., Horvathova, E., Measurement of DNA strand breakage and DNA repair induced with hydrogen peroxide using single cell gel electrophoresis, alkaline DNA unwinding and alkaline elution of DNA. Neoplasma 44 (1997), 380–383.
23. Melis, J.P., van Steeg, H., Luijten, M., Oxidative DNA damage and nucleotide excision repair. Antioxid. Redox Signal., 2012 December 7 (Epub ahead of print).
24. Altman, S.A., Zastawny, T.H., Randers, L., Lin, Z., Lumpkin, J.A., Remacle, J., Dizdaroglu, M., Rao, G., tert-Butyl hydroperoxide-mediated DNA base damage in cultured mammalian cells. Mutat. Res. 306 (1994), 35–44.
25. Lazze, M.C., Pizzala, R., Savio, M., Stivala, L.A., Prosperi, E., Bianchi, L., Anthocyanins protect against DNA damage induced by tert-butyl hydroperoxide in rat smooth muscle and hepatoma cells. Mutat. Res. 535 (2003), 103–115.
26. Slamenova, D., Horvathova, E., Chalupa, I., Wsolova, L., Navarova, J., Ex vivo assessment of protective effects of carvacrol against DNA lesions induced in primary rat cells by visible light excited methylene blue (VL + MB). Neoplasma 58 (2011), 14–19.
27. Aydin, S., Basaran, A.A., Basaran, N., The effects of thyme volatiles on the induction of DNA damage by the heterocyclic amine IQ and mitomycin C. Mutat. Res. 581 (2005), 43–53.
28. Koparal, A.T., Zeytinoglu, M., Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line A549. Biomed. Subject Life Sci. 43 (2003), 149–154.
29. Slamenova, D., Horvathova, E., Sramkova, M., Marsalkova, L., DNA-protective effects of two components of essential plant oils carvacrol and thymol on mammalian cells cultured in vitro. Neoplasma 54 (2007), 108–112.
30. Aherne, S.A., O'Brien, N.M., Mechanism of protection by the flavonoids, quercetin and rutin, against tert-butyl hydroperoxide- and menadione-induced DNA single strand breaks in Caco-2 cells. Free Radic. Biol. Med. 29 (2000), 507–514.
31. Chen, M., Gu, H., Ye, Y., Lin, B., Sun, L., Deng, W., Zhang, J., Liu, J., Protective effects of hesperidin against oxidative stress of tert-butyl hydroperoxide in human hepatocytes. Food Chem. Toxicol. 48 (2010), 2980–2987.
32. Hwang, J.M., Wang, C.J., Chou, F.P., Tseng, T.H., Hsieh, Y.S., Lin, W.L., Chu, C.Y., Inhibitory effect of berberine on tert-butyl hydroperoxide-induced oxidative damage in rat liver. Arch. Toxicol. 76 (2002), 664–670.
33. Hwang, J.M., Wang, C.J., Chou, F.P., Tseng, T.H., Hsieh, Y.S., Hsu, J.D., Chu, C.Y., Protective effect of baicalin on tert-butyl hydroperoxide-induced rat hepatotoxicity. Arch. Toxicol. 79 (2005), 102–109.
34. Lima, C.F., Fernandes-Ferreira, M., Pereira-Wilson, C., Phenolic compounds protect HepG2 cells from oxidative damage: relevance of glutathione levels. Life Sci. 79 (2006), 2056–2068.
35. Alia, M., Mateos, R., Ramos, S., Lecumberri, E., Bravo, L., Goya, L., Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2). Eur. J. Nutr. 45 (2006), 19–28.
36. Goya, L., Mateos, R., Bravo, L., Effect of the olive oil phenol hydroxytyrosol on human hepatoma HepG2 cells; protection against oxidative stress induced by tert-butyl hydroperoxide. Eur. J. Nutr. 46 (2007), 70–78.
37. Sohn, J.H., Han, K.L., Choo, J.H., Hwang, J.K., Macelignan protects HepG2 cells against tert-butyl hydroperoxide-induced oxidative damage. Biofactors 29 (2007), 1–10.
38. Lee, K.J., Choi, J.H., Hwang, Y.P., Chung, Y.C., Jeong, H.G., Protective effect of caffeic acid phenethyl ester on tert-butyl hydroperoxide-induced oxidative hepatotoxicity and DNA damage. Food Chem. Toxicol. 46 (2008), 2445–2450.
39. Vidyashankar, S.K., Mitra, S., Nandakumar, K.S., Liv.52 protects HepG2 cells from oxidative damage induced by tert-butyl hydroperoxide. Mol. Cell Biochem. 333 (2010), 41–48.
40. Noh, J.R., Gang, G.T., Kim, Y.H., Yang, K.J., Hwang, J.H., Lee, H.S., Oh, W.K., Song, K.S., Lee, C.H., Antioxidant effects of the chestnut (Castanea crenata) inner shell extract in t-BHP-treated HepG2 cells, and CCl4- and high-fat diet-treated mice. Food Chem. Toxicol. 48 (2010), 3177–3183.
41. Krall, J., Bagley, A.C., Mullenbach, G.T., Hallewell, R.A., Lynch, R.E., Superoxide mediates the toxicity of paraquat for cultured mammalian cells. J. Biol. Chem. 263 (1988), 1910–1914.
42. Alia, M., Ramos, S., Mateos, R., Bravo, L., Goy, L., Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J. Biochem. Mol. Toxicol. 19 (2005), 119–128.
43. Aristatile, B., Al-Numair, K.S., Veeramani, C., Pugalendi, K., Effect of carvacrol on hepatic marker enzymes and antioxidant status in D-galactosamine-induced hepatotoxicity in rats. Fundam. Clin. Pharmacol. 23 (2009), 757–765.
44. Yildirim, A., Mavi, A., Kara, A.A., Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J. Agric. Food Chem. 49 (2001), 4083–4089.
45. Espin, J., Soler-Rivas, C., Wichers, H.J., Characterization of the total free radical scavenging capacity of vegetable oil fractions using 2,2-diphenyl-1-picrylhydrazil radical. J. Agric. Food Chem. 48 (2000), 648–656.
46. Collins, A.R., Horvathova, E., Oxidative DNA damage, antioxidants and DNA repair: applications of the comet assay. Biochem. Soc. Trans. 29 (2001), 337–341.