A fluorescent microplate assay quantifies bacterial efflux and demonstrates two distinct compound binding sites in AcrB

Antimicrobial Agents and Chemotherapy

Volumn 59, Issue 4, 2015, Pages 2388-2397

  Iyer, Ramkumar ^a   Ferrari, Annette ^b   Rijnbrand, R ^c   Erwin, Alice L ^c  

^a VERTEX PHARMACEUTICALS   (United States)

^b NOVARTIS VACCINES AND DIAGNOSTICS   (United States)

^c Erwin Consulting ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACRB PROTEIN; AZITHROMYCIN; BACTERIAL PROTEIN; BIS (1,3 DIBUTYLBARBITURIC ACID) TRIMETHINE OXONOL; CARBON NITROGEN OXYGEN MONOCYCLE; CHLORTETRACYCLINE; CLARITHROMYCIN; CLOTRIMAZOLE; DEMECLOCYCLINE; DOXYCYCLINE; DYE; ECONAZOLE; ERYTHROMYCIN; FLUCONAZOLE; FLUORESCENT DYE; JOSAMYCIN; KETOCONAZOLE; MACROLIDE; MECLOCYCLINE; METACYCLINE; MICONAZOLE; MINOCYCLINE; NILE RED; OXYTETRACYCLINE; SPIRAMYCIN; SULCONAZOLE; TETRACYCLINE; TETRACYCLINE DERIVATIVE; TIGECYCLINE; TYLOSIN; UNCLASSIFIED DRUG; ACRB PROTEIN, E COLI; ANTIINFECTIVE AGENT; ESCHERICHIA COLI PROTEIN; MULTIDRUG RESISTANCE PROTEIN; OXAZINE DERIVATIVE;

ANTIBACTERIAL ACTIVITY; ARTICLE; ASSAY; BACTERIAL EFFLUX; BACTERIAL GROWTH; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIAL STRAIN; BINDING SITE; CHEMICAL STRUCTURE; CRYSTALLOGRAPHY; EC50; ENZYME SPECIFICITY; ESCHERICHIA COLI; FLUORESCENT MICROPLATE ASSAY; GROWTH INHIBITION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; ALGORITHM; BINDING COMPETITION; CHEMISTRY; METABOLISM; MICROBIAL SENSITIVITY TEST; MOLECULAR WEIGHT;

ALGORITHMS; ANTI-BACTERIAL AGENTS; BINDING SITES; BINDING, COMPETITIVE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; MICROBIAL SENSITIVITY TESTS; MOLECULAR WEIGHT; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; OXAZINES;

EID: 84928911574     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.05112-14     Document Type: Article

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